Identification of drug target for novel antipsychotics based on proteomic approach
| Project/Area Number |
21790067
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Nagoya University |
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Principal Investigator |
NAGAI Taku Nagoya University, 医学部附属病院, 准教授 (10377426)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 薬理学 / 脳・神経 / 神経化学 / 基礎研究 / 蛋白質 / プロテオーム / 統合失調症 / グルタミン酸受容体 / 神経科学 |
|---|
| Research Abstract |
Repeated administration of phencyclidine (PCP) has known to produces schizophrenic-like symptoms in mice. To examine the changes in protein expression after repeated PCP treatment in the prefrontal cortex (PFC) of mice, we performed proteomic analysis by using 2D-DIGE. Proteomic analysis reveals that the remarkable change in the expression levels of 14-3-3 protein θ was observed in the PFC after repeated treatment with PCP. The signals was localized to cells positive for S100, a marker of glial cells, in the PFC. Microinjection of 14-3-3 protein inhibitor into the PFC lead to memory impairment in a novel object recognition test. These results suggest that dysfunction of 14-3-3 protein θ in the PFC may be involved in the molecular mechanisms underlying the schizophrenic-like symptoms in repeated PCP-treated mice.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Chronic restraint stress impairs neurogenesis and hippocampus-dependent fear memory in mice2010
Author(s)
Yun, J., Koike, H., Ibi, D., Toth, E., Mizoguchi, H., Nitta, A., Yoneyama, M., Ogita, K.Yoneda, Y., Nabeshima, T., Nagai, T., Yamada, K.
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Journal Title
Possible involvement of a brain-specific transcription factor Npas4.J.Neurochem 114
Pages: 1840-1851
Related Report
Peer Reviewed
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[Journal Article] Nicotine ameliorates emotional and cognitive impairments induced by neonatal polyI2010
Author(s)
Yu, J., Nagai, T., Ibi, D., Kitahara, Y., Nabeshima, T., Yamada, K.
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Journal Title
C treatment in mice.Open Behav.Sci.J. 4
Pages: 9-18
Related Report
Peer Reviewed
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[Journal Article] Dysfunction of dopamine release in the prefrontal cortex of dysbindin deficient sandy mice2010
Author(s)
Nagai, T., Kitahara, Y., Shiraki, A., Hikita, T., Taya, S., Kaibuchi, K., Yamada, K.
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Journal Title
an in vivo microdialysis study.Neurosci.Lett 470
Pages: 134-138
Related Report
Peer Reviewed
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[Journal Article] Aripiprazole ameliorates phencyclidine-induced impairment of recognition memory through dopamine D1 and serotonin 5-HT1A receptors.2009
Author(s)
Nagai, T., Murai, R., Matsui, K., Kamei, H., Noda, Y., Furukawa, H., Nabeshima, T.
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Journal Title
Psychopharmacology 202
Pages: 315-328
Related Report
Peer Reviewed
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[Presentation] Neonatal polyI:C treatment in mice induces schizophrenia-like behavioral and neurochemical abnormalities in adulthood.2009
Author(s)
Nagai, T., Ibi, D., Mizoguchi, H., Nabeshima, T., Yamada, K.
Organizer
Neuroscience 2009, the 39th annual meeting of the Society for Neuroscience.
Place of Presentation
Chicago, USA
Related Report
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