Research Project
Grant-in-Aid for Young Scientists (B)
Repeated administration of phencyclidine (PCP) has known to produces schizophrenic-like symptoms in mice. To examine the changes in protein expression after repeated PCP treatment in the prefrontal cortex (PFC) of mice, we performed proteomic analysis by using 2D-DIGE. Proteomic analysis reveals that the remarkable change in the expression levels of 14-3-3 protein θ was observed in the PFC after repeated treatment with PCP. The signals was localized to cells positive for S100, a marker of glial cells, in the PFC. Microinjection of 14-3-3 protein inhibitor into the PFC lead to memory impairment in a novel object recognition test. These results suggest that dysfunction of 14-3-3 protein θ in the PFC may be involved in the molecular mechanisms underlying the schizophrenic-like symptoms in repeated PCP-treated mice.
All 2010 2009 Other
All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (10 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)
Possible involvement of a brain-specific transcription factor Npas4.J.Neurochem 114
Pages: 1840-1851
C treatment in mice.Open Behav.Sci.J. 4
Pages: 9-18
an in vivo microdialysis study.Neurosci.Lett 470
Pages: 134-138
Neurosci.Lett.
Volume: 470 Pages: 134-138
Open Behav.Sci.J.
Volume: 4 Pages: 9-18
J.Neurochem.
Volume: 114 Pages: 1840-1851
Neurosci. Lett. 470
Psychopharmacology 202
Pages: 315-328
http://www.med.nagoya-u.ac.jp/pharmacy/02/index.html
