Physiological role of SCN micro-circuit on circadian clock

• Project/Area Number: 21790073
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Kyoto University
• Principal Investigator: YAMAGUCHI Yoshiaki Kyoto University, 薬学研究科, 助教 (30467427)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 体内時計 / 視交叉上核 / グルタミン酸受容体 / 時計遺伝子 / サーカディアンリズム / AMPA受容体 / Per / 概日リズム / アラトスタチン / 神経機能抑制 / リズム同調 / マウス / アデノ随伴ウイルス

Research Abstract

The glutamatergic neurotransmission in the suprachiasmatic nucleus (SCN) plays a central role in the entrainment of the circadian rhythms to environmental light-dark cycles. Although the glutamatergic effect operating via NMDAR is well elucidated, much less is known about a role of AMPAR in circadian entrainment. Here I show that in vivo microinjection of AMPA in the SCN during the early subjective night phase-delays the behavioral rhythm. These data demonstrate that activation of AMPAR is capable of phase-shifting the circadian clock both in vivo and in vitro, and are consistent with the hypothesis that activation of AMPA receptors is a critical step in the transmission of photic information to the SCN.

Research Products

• [Journal Article] Hypertension due to loss of clock : novel insight from the molecular analysis of cry1/cry2-deleted mice. (2011)
  • Author(s): Okamura H, Doi M, Yamaguchi Y, Fustin JM.
  • Journal Title: Curr Hypertens Rep 13 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Transportin 1 in the mouse brain : Appearance in regions of neurogenesis, cerebrospinal fluid production/sensing, and circadian clock. (2011)
  • Author(s): Sato M, Mizoro Y, Atobe Y, Fujimoto Y, Yamaguchi Y, Fustin JM, Doi M, Okamura H.
  • Journal Title: Journal of Comparative Neurology 519 Pages: 1770-1780
  • Peer Reviewed
• [Journal Article] Circadian regulation of intracellular G-protein signalling mediates intercellular synchrony and rhythmicity in the suprachiasmatic nucleus. (2011)
  • Author(s): Doi M, Ishida A, Miyake A, Sato M, Komatsu R, Yamazaki F, Kimura I, Tsuchiya S, Kori H, Seo K, Yamaguchi Y, Matsuo M, Fustin JM, Tanaka R, Santo Y, Yamada H, Takahashi Y, Araki M, Nakao K, Aizawa S, Kobayashi M, Obrietan K, Tsujimoto G, Okamura H.
  • Journal Title: Nat Commun 2 Pages: 327-327
  • NAID: 120003056234
  • Peer Reviewed
• [Journal Article] Hypertension due to loss of clock : novel insight from the molecular analysis of cry1/cry2-deleted mice (2011)
  • Author(s): Okamura H, Doi M, Yamaguchi Y, Fustin JM
  • Journal Title: Curr Hypertens Rep Volume: 13 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Mammalian circadian clock system : Molecular mechanisms for pharmaceutical and medical sciences. (2010)
  • Author(s): Okamura H, Doi M, Fustin JM, Yamaguchi Y, Matsuo M.
  • Journal Title: Advanced Drug Delivery Reviews 62 Pages: 876-884
  • Peer Reviewed
• [Journal Article] Activation of AMPA receptors in the suprachiasmatic nucleus phase-shifts the mouse circadian clock in vivo and in vitro. (2010)
  • Author(s): Mizoro Y, Yamaguchi Y, Kitazawa R, Yamada H, Matsuo M, Fustin JM, Doi M, Okamura H.
  • Journal Title: PLoS One 5
  • Peer Reviewed
• [Journal Article] Activation of AMPA receptors in the suprachiasmatic nucleus phase-shifts the mouse circadian clock in vivo and in vitro (2010)
  • Author(s): Mizoro Y, Yamaguchi Y, Kitazawa R, Yamada H, Matsuo M, Fustin JM, Doi M, Okamura H
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Mammalian circadian clock system : Molecular mechanisms for pharmaceutical and medical sciences (2010)
  • Author(s): Okamura H, Doi M, Fustin JM, Yamaguchi Y, Matsuo M
  • Journal Title: Advanced Drug Deli very Reviews Volume: 62 Pages: 876-884
  • Peer Reviewed
• [Journal Article] Molecular clocks in mouse skin. (2009)
  • Author(s): Tanioka M, Yamada H, Doi M, Bando H, Yamaguchi Y, Nishigori C, Okamura H.
  • Journal Title: Journal of Investigative Dermatology 129 Pages: 1225-1231
  • Peer Reviewed
• [Journal Article] Circadian expression of the Na+/H+ exchanger NHE3 in the mouse renal medulla. (2009)
  • Author(s): Nishinaga H, Komatsu R, Doi M, Fustin JM, Yamada H, Okura R, Yamaguchi Y, Matsuo M, Emoto N, Okamura H.
  • Journal Title: Biomedical Research 30 Pages: 87-93
  • NAID: 130004470742
  • Peer Reviewed
• [Journal Article] Circadian expression of the Na+/H+ exchanger NHE3 in the mouse renal medulla (2009)
  • Author(s): Nishinaga H., Komatsu R., Doi M., Fustin JM., Yamada H., Okura R., Yamaguchi Y., Matsuo M., Emoto N., Okamura H
  • Journal Title: Biomed Res 30 Pages: 87-93
  • NAID: 130004470742
  • Peer Reviewed
• [Journal Article] Molecular clocks in mouse skin (2009)
  • Author(s): Tanioka M, Yamada H, Doi M, Bando H, Yamaguchi Y, Nishigori C, Okamura H
  • Journal Title: J Invest Dermatol 129 Pages: 1225-1231
  • Peer Reviewed
• [Presentation] AMPA受容体シグナルによる体内時計調節機構 (2010)
  • Author(s): 山口賀章, 溝曽路祥孝, 岡村均
  • Organizer: 第60回日本薬学会近畿支部総会・大会
  • Place of Presentation: 摂南大学
  • Year and Date: 2010-10-30
• [Presentation] AMPA受容体シグナルによる体内時計調節機構 (2010)
  • Author(s): 山口賀章
  • Organizer: 第60回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 摂南大学(大阪)
  • Year and Date: 2010-10-30
• [Book] 季節変動と日内変動(体内時計とは? 体内時計について教えてください.Q&Aでわかる肥満と糖尿病9) (2010)
  • Author(s): 山口賀章, 岡村均
  • Publisher: 丹水社
• [Book] Q&Aでわかる肥満と糖尿病 (2010)
  • Author(s): 山口賀章, 岡村均
  • Total Pages: 3
  • Publisher: 丹水社
• [Book] Q&Aでわかる肥満と糖尿病-体内時計とは?体内時計について教えてください (2010)
  • Author(s): 山口賀章, 岡村均
  • Total Pages: 3
  • Publisher: (株)丹水社
• [Remarks] ホームページ等
  • URL: http://www.pharm.kyoto-u.ac.jp/system-biology/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/system-biology/