SH3P2 is a novel regulator of cell motility.

• Project/Area Number: 21790081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: TANIMURA Susumu Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (90343342)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞運動 / ERK-MAPキナーゼ / RSK / リン酸化 / ミオシン / 細胞膜ラッフリング / 細胞骨格 / シグナル伝達 / がん / 癌

Research Abstract

We have found that SH3P2, a novel negative regulator of cell motility binds to Myo1E, which dissociates from SH3P2 in an RSK1-mediated Ser^<202> phosphorylation-dependent manner and regulates the expression of CD44 and MMP-3/-9. These results suggest that SH3P2/Myo1E complex is an essential machinery that functions downstream of the ERK-MAP kinase pathway to modulate cell motility.

Research Products

• [Journal Article] SH3P2 is a negative regulator of cell motility whose function is inhibited by ribosomal S6 kinase-mediated phosphorylation (2011)
  • Author(s): Susumu Tanimura, Junya Hashizume, Yukiko Kurosaki, Kanako Sei, Aiko Gotoh, Rika Ohtake, Michihiro Kawano, Kazushi Watanabe, Michiaki Kohno
  • Journal Title: Genes Cells in press
  • Peer Reviewed
• [Journal Article] SH3P2 is a negative regulator of cell motility whose function is inhibited by ribosomal S6 kinase-mediated phosphorylation (2011)
  • Author(s): Tanimura S., et al.
  • Journal Title: Genes to Cells Volume: (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Blockade of the extracellular signal-regulated kinase pathway enhances the therapeutic efficacy of microtubule-destabilizing agents in human tumor xenograft model. (2010)
  • Author(s): Kazushi Watanabe, Susumu Tanimura, Aya Uchiyama, Toshiaki Sakamoto, Takumi Kawabata, Kei-ichi Ozaki, Michiaki Kohno
  • Journal Title: Clin.Cancer Res. 16 Pages: 1170-1178
  • Peer Reviewed
• [Journal Article] Blockade of the ERK or PI3K-Akt signaling pathway enhances the cytotoxicity of histone deacetylase inhibitors in tumor cells resistant to gefitinib or imatinib (2010)
  • Author(s): Kei-ichi Ozaki, Masaki Kosugi, Nobuyuki Baba, Kohsuke Fujio, Toshiaki Sakamoto, Shinya Kimura, Susumu Tanimura, Michiaki Kohno
  • Journal Title: Biochem.Biophys.Res.Commun. 391 Pages: 1610-1615
  • NAID: 120006983583
  • Peer Reviewed
• [Journal Article] unprecedented NES non-antagonistic inhibitor for nuclear export of MEK. (2010)
  • Author(s): Satoru Tamura, Yuuhi Hattori, Masafumi Kaneko, Nobuhiro Shimizu, Susumu Tanimura, Michiaki Kohno, Nobutoshi Murakami, Peumusolide A
  • Journal Title: Tetrahedron Lett. 51 Pages: 1678-1681
  • Peer Reviewed
• [Journal Article] Blockade of the extracellular signal-regulated kinase pathway enhances the therapeutic efficacy of microtubule-destabilizing agents in human tumor xenograft modl. (2010)
  • Author(s): Watanabe K.
  • Journal Title: Clin.Cancer Ther. 16 Pages: 1170-1178
  • Peer Reviewed
• [Journal Article] Peumusolide A, unprecedented NES non-antagonistic inhibitor for nuclear export of MEK. (2010)
  • Author(s): Tamura S.
  • Journal Title: Tetrahedron Lett. 51 Pages: 1678-1681
  • Peer Reviewed
• [Journal Article] ERK Regulates Renal Cell Proliferation and Renal Cyst Expansion in inv Mutant Mice. (2009)
  • Author(s): Yasuko Okumura, Noriyuki Sugiyama, Susumu Tanimura, Masashi Nishida, Kenji Hamaoka, Michiaki Kohno, Takahiko Yokoyama
  • Journal Title: Acta.Histochem.Cytochem. 42 Pages: 39-45
  • NAID: 130000114963
  • Peer Reviewed
• [Journal Article] GEF-H1 mediates tumor necrosis factor-α-induced Rho activation and myosin phosphorylation : role in the regulation of tubular paracellular permeability. (2009)
  • Author(s): Eli Kakiashvili, Pam Speight, Faiza Waheed, Romy Seth, Monika Lodyga, Susumu Tanimura, Michiaki Kohno, Ori D.Rotstein, Andra's Kapus, Katalin Sza' szi
  • Journal Title: J.Biol.Chem. 284 Pages: 11454-11466
  • Peer Reviewed
• [Journal Article] GEF-H1 mediates tumor necrosis factor-α-induced Rho activation and myosin phosphorylation : role in the regulation of tubular paracellular permeability. (2009)
  • Author(s): Kakiashvili E.
  • Journal Title: J.Biol.Chem 284 Pages: 11454-11466
  • Peer Reviewed
• [Presentation] 新規細胞運動制御因子SH3P2はMyosin1Eと結合する (2010)
  • Author(s): 河野通宏、橋詰淳哉、森田和幹、金丸雄祐、谷村進、河野通明
  • Organizer: 第27回日本薬学会九州支部大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2010-12-11
• [Presentation] Myosin1Eによる細胞運動制御の分子機構 (2010)
  • Author(s): 大竹里佳、橋詰淳哉、中原康子、谷村進、河野通明
  • Organizer: 第27回日本薬学会九州支部大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2010-12-11
• [Presentation] 新規細胞運動制御因子SH3P2はMyosin 1Eと結合する (2010)
  • Author(s): 河野通宏
  • Organizer: 第27回 日本薬学会九州支部大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2010-12-11
• [Presentation] Myosin 1Eによる細胞運動制御の分子機構 (2010)
  • Author(s): 大竹里佳
  • Organizer: 第27回 日本薬学会九州支部大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2010-12-11
• [Presentation] 新規タンパク質SH3P2による細胞運動制御の分子機構 (2010)
  • Author(s): 谷村進、大竹里佳、河野通宏、橋詰淳哉、後藤愛依子、黒崎由希子、瀬井香奈子、河野通明
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 新規タンパク質SH3P2による細胞運動制御の分子機構 (2010)
  • Author(s): 谷村進
  • Organizer: 第83回 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Myosin1Eはinvadopodiaの形成を介してがん細胞の浸潤を調節する (2010)
  • Author(s): 谷村進、橋詰淳哉、後藤愛依子、河野通宏、大竹里佳、渡邊一石、河野通明
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場、リーガロイヤルホテル大阪
  • Year and Date: 2010-09-23
• [Presentation] Myosin 1Eはinvadopodiaの形成を介してがん細胞の浸潤を調節する (2010)
  • Author(s): 谷村進
  • Organizer: 第69回 日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場リーガロイヤルホテル大阪
  • Year and Date: 2010-09-23
• [Presentation] SH3P2とMyosin1Eの相互作用による細胞運動・浸潤の制御 (2009)
  • Author(s): 後藤愛依子、大竹里佳、河野通宏、橋詰淳哉、谷村進、河野通明
  • Organizer: 第24回日本薬学会九州支部大会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-12-13
• [Presentation] SH3P2はMyosin 1Eの相互作用による細胞運動・浸潤の制御 (2009)
  • Author(s): 後藤愛依子
  • Organizer: 第24回日本薬学会九州支部大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-13
• [Presentation] SH3P2はMyo1Eの機能調節を介して細胞運動を制御する (2009)
  • Author(s): 橋詰淳哉、後藤愛依子、大竹里佳、瀬井香奈子、黒崎由希子、谷村進、河野通明
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] SH3P2はMyosin 1Eの機能調節を介して細胞運動を制御する (2009)
  • Author(s): 橋詰淳哉
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] SH3P2はMyosin1Eの機能抑制を介して細胞運動を阻害する (2009)
  • Author(s): 谷村進、橋詰淳哉、後藤愛依子、渡邊一石、河野通明
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-03
• [Presentation] SH3P2はMyosin 1Eの機能抑制を介して細胞運動を阻害する (2009)
  • Author(s): 谷村進
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03