| Project/Area Number |
21790082
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
YOSHINAGA Sosuke Kumamoto University, 大学院・生命科学研究部, 助教 (00448515)
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| Research Collaborator |
TERASHIMA Yuya 東京大学, 大学院・医学系研究科, 客員研究員
TERASAWA Hiroaki 熊本大学, 大学院・生命科学研究部, 教授 (10300956)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ケモカイン受容体 / NMR / 構造生物学 / 炎症性免疫疾患 / 創薬 |
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| Research Abstract |
We succeeded in constructing a protein expression system for a novel intracellular factor FROUNT that regulates a chemokine receptor CCR2b using Escherichia coli cells and developing a protocol for the protein purification. Utilizing this system and protocol, we obtained large amounts of purified full-length FROUNT proteins that are enough for structural analyses. We elucidated that the purified FROUNT proteins possess CCR2b-binding activity with solution NMR and surface plasmon resonance. We also succeeded in constructing a protein expression system of full-length CCR2b using insect cells and developing a protocol for the protein purification.
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