| Project/Area Number |
21790092
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Showa University |
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Principal Investigator |
OBAMA Takashi Showa University, 薬学部, 助教 (60395647)
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| Co-Investigator(Renkei-kenkyūsha) |
ITABE Hiroyuki 昭和大学, 薬学部, 教授 (30203079)
KATO Rina 昭和大学, 薬学部, 助教 (30392400)
木庭 新治 昭和大学, 医学部, 講師 (20276546)
KOBAYASHI Yoichi 昭和大学, 医学部, 教授 (70158905)
IWAMOTO Sanju 昭和大学, 医学部, 准教授
MIYAZAKI Akira 昭和大学, 医学部, 教授 (70253721)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 動脈硬化症 / 酸化LDL / プロテオーム / モノクローナル抗体 / 酸化ストレス / リポタンパク質 / LDL |
|---|
| Research Abstract |
To explore modifications in oxidized low-density lipoprotein (OxLDL) formed in vivo, we analyzed LDL fractions obtained by cardiac catheterization from patients with coronary artery disease. OxLDL was enriched by immunoprecipitation using monoclonal antibody against oxidized phosphatidylcholine (oxPC). Western blot analysis revealed that the OxLDL in vivo was modified by lipid peroxidation products such as 4-hydroxynonenal, acrolein and oxPC, but showed a different pattern from Cu^<2+>-induced OxLDL. These data suggest the structural aspects of oxLDL in vivo different from in vitro oxidation.
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