| Project/Area Number |
21790111
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
KOHNO Masayuki Kyoto University, 大学院・医学研究科, 客員研究員 (00437203)
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| Research Collaborator |
HORIBE Tomohisa 京都大学, 大学院・医学研究科, 特定助教 (20467468)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 医薬分子設計 / ペプチド創薬 / 分子標的 / 局所投与 / DDS |
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| Research Abstract |
We investigated the possibility of new powerful therapy for inflammatory diseases using chimera peptides that have a binding sequence to inflammatory cytokine receptors and an adhesion sequence to cell membrane without cytotoxic activity.
As a result of pre-survey, we selected TNFα, TLR4, and TGFβ1 as the target molecules, and determined the promising candidates among various antagonist peptides and chimera peptides by the in vitro assay systems. Inhibitory effect of TNFα-antagonist peptide was increased by chimera conjugation with a membrane-adhesion sequence. Local treatment of TGFβ1-antagonist peptide by intratracheal nebulization decreased the levels of fibrotic factors in lung tissue in bleomycin-induced lung fibrosis rat model.
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