Development and application of site specific fluorescent labeling of proteins
| Project/Area Number |
21790115
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | Nagoya City University |
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Principal Investigator |
UMEZAWA Naoki Nagoya City University, 大学院・薬学研究科, 准教授 (40347422)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 医薬分子機能学 / 生体機能関連化学 / 蛍光標識 / ペプチド / タンパク質 / 蛍光色素 / 金属錯体 |
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| Research Abstract |
We have developed "turn-on" fluorescent probes for labeling of His-tag ((His)_6), which later turned out to have some problems. The purpose of this research is the clarification and solution of these problems, to develop useful methodology for fluorescently label proteins. The main problem of our probes is the weak binding to His-tag without N-terminal primary amino group. We determined the problem is mainly due to the His-tag recognition site, a metal complex. We explored the better His-tag recognition site and identified a metal-complex, which can bind to His-tag at any position.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article]2010
Author(s)
Naoki Umezawa, Nobuyoshi Matsumoto, Shinsuke Iwama, Nobuki Kato, Tsunehiko Higuchi
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Journal Title
Bioorg. Med. Chem. 18(17)
Pages: 6340-6350
Related Report
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[Journal Article]2010
Author(s)
Shinya Mimasu, Naoki Umezawa, Shin Sato, Tsunehiko Higuchi, Takashi Umehara, Shigeyuki Yokoyama
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Journal Title
Biochemistry 49(30)
Pages: 6494-6503
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[Presentation]
Author(s)
梅澤直樹
Organizer
日本薬学会第131年会、S31-6
Place of Presentation
静岡
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