Elucidation of drug resistance mechanism indicated by DNA methylation in colon cancer

• Project/Area Number: 21790140
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: University of Tsukuba
• Principal Investigator: SUZUKI Hideo 筑波大学, 医学医療系, 准教授 (00400672)
• Research Collaborator: WATANABE Yoshiyuki 聖マリアンナ医科大学, 消化器肝臓内科, 講師 (90329243)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: オーダーメード医療 / DNAメチル化 / 消化器癌 / 化学療法 / 内視鏡的治療 / 内視鏡治療 / 癌

Research Abstract

(1)The SNP of EGFR D944D in Exon25 was statistically correlated with the expression of skin toxicity in unresectable colorectal cancer patients administrated anti-EGFR antibody, suggesting that application to personalized medication that enables the prediction of this side effect.
(2)By comprehensive analysis of DNA methylation in gastric washes before and after endoscopic treatment, methylation in MINT25 and SOX17 gene was reduced significantlyafter treatment with curative resection in early gastric cancer patients. This method may be useful for early detection of recurrence after endoscopic treatment.

Research Products

• [Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer. (2012)
  • Author(s): Watanabe Y et al
  • Journal Title: Tumour Biol Volume: 33(2) Issue: 2 Pages: 383-93
  • DOI: 10.1007/s13277-011-0278-y
  • Peer Reviewed
• [Journal Article] Genome-scale DNA methylation analysis in GI cancers (2012)
  • Author(s): 渡邊嘉行、鈴木英雄
  • Journal Title: SEIBUTSU BUTSURI KAGAKU Volume: 56 Issue: 1 Pages: 5-8
  • DOI: 10.2198/sbk.56.5
  • NAID: 130004456514
  • ISSN: 0031-9082, 1349-9785
  • Peer Reviewed
• [Journal Article] DNA Methylation Profiles of Primary Colorectal Carcinoma and Matched Liver Metastasis (2011)
  • Author(s): Watanabe Y, et al.
  • Journal Title: PLoS One Volume: 6 Issue: 11 Pages: e27889-e27889
  • DOI: 10.1371/journal.pone.0027889
  • Peer Reviewed
• [Journal Article] DNA methylation of interferon regulatory factors in gastric cancer and noncancerous gastric mucosae. (2010)
  • Author(s): Yamashita M, Watanabe Y, et al.
  • Journal Title: Cancer Sci. Volume: 101 Issue: 7 Pages: 1708-1716
  • DOI: 10.1111/j.1349-7006.2010.01581.x
  • NAID: 10026594987
  • Peer Reviewed
• [Journal Article] DNA methylation of interferon regulatory factors in gastric cancer and noncancerous gastric mucosae. (2010)
  • Author(s): Yamashita M, Watanabe Y, et al.
  • Journal Title: Cancer Science Volume: 101 Pages: 1708-1716
  • NAID: 10026594987
  • Peer Reviewed
• [Presentation] 胃洗浄廃液を用いた胃がんメチル化マーカーの探索 (2012)
  • Author(s): 渡邊嘉行
  • Organizer: 第23回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-16
• [Presentation] The Sox17 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Hama R, Watanabe Y et al.
  • Organizer: American Association for Cancer Research 2012
  • Place of Presentation: Chicago IL.(USA)
  • Year and Date: 2012-04-03
• [Presentation] 胃洗浄廃液を用いた胃がんメチル化マーカーの探索 (2012)
  • Author(s): 渡邊嘉行
  • Organizer: 第２３回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
• [Presentation] 早期胃がんESD後補助診断としての胃洗浄廃液診断の有用性 (2011)
  • Author(s): 渡邊嘉行, ら
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] Usefulness of DNA methylation analysis using gastric washes as a treatment marker for early gastric cancer (2011)
  • Author(s): Watanabe Y et al
  • Organizer: American Association for Cancer Research 2011
  • Place of Presentation: Orland FL.(USA)
  • Year and Date: 2011-04-03
• [Presentation] Usefulness of DNA methylation analysis using gastric washes as a treatment marker for early gastric cancer (2011)
  • Author(s): Watanabe Y, et al.
  • Organizer: American Association for Cancer Research
  • Place of Presentation: Orland (USA)
• [Presentation] Usefulness of DNA methylation analysis using gastric washes as a treatment marker for early gastric cancer (2011)
  • Author(s): Watanabe Y, et al.
  • Organizer: Digestive Disease Week
  • Place of Presentation: Chicago (USA)
• [Presentation] 消化器内視鏡検査と分子生物学的診断の新たな展開 (2010)
  • Author(s): 細谷浩介、渡邊嘉行
  • Organizer: JDDW2010シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-14
• [Presentation] 消化器内視鏡検査と分子生物学的診断の新たな展開 (2010)
  • Author(s): 細谷浩介、渡邊嘉行, 他
  • Organizer: JDDW2010 シンポジウム
  • Place of Presentation: パシフィコ横浜展示ホール
  • Year and Date: 2010-10-14
• [Book] エピジェネティックと大腸癌 (2011)
  • Author(s): 渡邊嘉行
  • Total Pages: 6
  • Publisher: BIO Clinica
• [Patent(Industrial Property Rights)] 胃粘膜洗浄液を利用した疾患関連マーカー検出方法 (2007)
  • Inventor(s): 学校法人聖マリアンナ医科大学、北海道公立大学法人札幌医科大学
  • Industrial Property Rights Holder: 渡邊 嘉行、豊 田 実、今 井 浩三、篠村 恭久、伊東 文生、時野 隆至
  • Filing Date: 2007-05-15
  • Overseas