Epigenetic regulation of CYP3A4 gene and individual phenotypic status

Research Project

Project/Area Number	21790151
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Medical pharmacy
Research Institution	Kyushu University
Principal Investigator	HIROTA Takeshi Kyushu University, 薬学研究院, 助教 (80423573)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords	エピジェネティクス / 発現制御 / 薬物代謝酵素 / バイオマーカー
Research Abstract	We show that DNA methylation in CYP3A4 enhancer region is critically involved in determining the individual hepatic CYP3A4 mRNA level. Chromosome Conformation Capture assay suggested long-range cis-interaction between the enhancer region and the transcription start site.