| Project/Area Number |
21790152
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
WATANABE Hiroshi Kumamoto University, 薬学部, 講師 (70398220)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | コレステロール / トランスポータ / 代謝酵素 / 体内動態 / 腎不全 / スタチン / 個別化投与設計 |
|---|
| Research Abstract |
We show that, when parental Chinese hamster ovary (CHO) cells are briefly exposed to ^3H-cholesteryl linoleate (CL) labeled-LDL, newly liberated ^3H-LDL-CHOL appears in membranes rich in trans-Golgi network (TGN) long before it becomes available for re-esterification at the endoplasmic reticulum (ER) or for efflux at the plasma membrane. In mutant cells lacking NPC1, the appearance of newly liberated ^3H-LDL-CHOL in the TGN-rich fractions is much reduced. We demonstrate that knockdown by RNAi of 3 TGN-specific SNAREs (VAMP4, syntaxin 6, and syntaxin 16) reduces >/=50% of the LDL-CHOL transport in intact cells and in vitro.
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