Development of novel assay system for the functionality of organic cation transporters using fluorescent probe substrate
Project/Area Number |
21790155
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Medical pharmacy
|
Research Institution | Nagoya City University |
Principal Investigator |
OHTA Kinya Nagoya City University, 大学院・薬学研究科, 助教 (90448704)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 医療薬剤学 / トランスポーター / 有機カチオン / 蛍光 / スクリーニング / 薬物間相互作用 / 薬物動態 |
Research Abstract |
We successfully demonstrated that DAPI can be efficiently transported by both hMATEs, hMATE1 and hMATE2-K, and hOCT1. By taking advantage of its fluorescent nature, we developed a rapid assay system for the selection of inhibitors, which include potential substrates, of hMATEs and hOCT1.
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Report
(3 results)
Research Products
(13 results)