Exploration of novel biomarker for diagnosis of early stage of arteriosclerosis

• Project/Area Number: 21790156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: IWAKI Soichiro 名古屋市立大学, 大学院・薬学研究科, 助教 (60399962)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 動脈硬化症 / バイオマーカー / miRNA / PAI-1 / スフィンゴシン1-リン酸

Research Abstract

We performed a comprehensive analysis of miRNA expression in the aorta of wild-type and ApoE^<-/-> mice using miRNA expression microarrays, total of 12 miRNAs were identified to be differentially expressed. We also demonstrated here that miR-126 levels in plasma are altered in the coronary artery disease patients. These results suggest that miRNAs are available as a novel biomarker to assess the early stage of arteriosclerosis.

Research Products

• [Journal Article] Role of down-regulated neutral ceramidase during all-trans retinoic acid induced neuronal differentiation in SH-SY5Y neuroblastoma cells. (2012)
  • Author(s): Kouji Tanaka, Keiko Tamiya-Koizumi, Kazumi Hagiwara, Hiromi Ito, Akira Takagi, Tetsuhito Kojima, Motoshi Suzuki, Soichiro Iwaki, Satoshi Fujii, Mitsuhiro Nakamura, Yoshiko Banno, Reiji Kannagi, Tatsuya Tsurumi, Mamoru Kyogashima, Takashi Murate.
  • Journal Title: J. Biochem. Volume: (in press)
  • NAID: 10031166103
  • Peer Reviewed
• [Journal Article] Analytical evaluation of plasma serotonin and sphingosine 1-phosphate and their clinical assessment in early atherosclerosis. (2012)
  • Author(s): Tomonori Sugiura, Yasuaki Dohi, Sumiyo Yamashita, Nobuyuki Ohte, Shiori Ito, Soichiro Iwaki, Yuji Hirowatari, Ryunosuke Ohkawa, Yuko Mishima, Yutaka Yatomi, Genjiro Kimura, Satoshi Fujii.
  • Journal Title: Coron. Artery Dis. Volume: 23 Pages: 234-238
  • Peer Reviewed
• [Journal Article] Analytical evaluation of plasma serotonin and sphingosine 1-phosphate and their clinical assessment in early atherosclerosis (2012)
  • Author(s): Tomonori Sugiura
  • Journal Title: Coron.Artery Dis. Volume: (印刷中) Issue: 4 Pages: 234-238
  • DOI: 10.1097/mca.0b013e328351ab0a
  • Peer Reviewed
• [Journal Article] Role of down-regulated neutral ceramidase during all-trans retinoic acid induced neuronal differentiation in SH-SY5Y neuroblastoma cells (2012)
  • Author(s): Kouji Tanaka
  • Journal Title: J.Biochem. Volume: (印刷中) Issue: 6 Pages: 611-620
  • DOI: 10.1093/jb/mvs033
  • Peer Reviewed
• [Journal Article] Modulators of induction of plasminogen activator inhibitor type-1 in HepG2 cells by transforming growth factor-β (2011)
  • Author(s): Naomi Nakayama, Tomomi Nakamura, Hiromi Okada, Soichiro Iwaki, Burton E. Sobel, Satoshi Fujii
  • Journal Title: Coron. Artery Dis Volume: 22 Pages: 468-78
  • Peer Reviewed
• [Journal Article] Posttranscriptional regulation of expression of plasminogen activator inhibitor type-1 by cAMP in HepG2 liver cells (2011)
  • Author(s): Takefumi Asakura, Soichiro Iwaki, Hiromi Okada, Burton E. Sobel, Satoshi Fujii
  • Journal Title: J. Biochem. Volume: 150 Pages: 687-694
  • NAID: 10030589369
  • Peer Reviewed
• [Journal Article] Posttranscriptional regulation of expression of plasminogen activator inhibitor type-1 by cAMP in HepG2 liver cells (2011)
  • Author(s): Takefumi Asakura
  • Journal Title: J.Biochem. Volume: 150 Issue: 6 Pages: 687-694
  • DOI: 10.1093/jb/mvr110
  • Peer Reviewed
• [Journal Article] Modulators of induction of plasminogen activator inhibitor type-1 in HepG2 cells by transforming growth factor-β (2011)
  • Author(s): Naomi Nakayama
  • Journal Title: Coron.Artery Dis. Volume: 22 Issue: 7 Pages: 468-478
  • DOI: 10.1097/mca.0b013e32834a3817
  • Peer Reviewed
• [Journal Article] HepG2細胞におけるインスリンと酸化ストレスのPAI-1産生に対する影響の解析:メタボリックシンドロームの易血栓性の病態解明を指向して (2010)
  • Author(s): 宮川隆, 朝倉健文, 中村友美, 佐藤由樹, 岡田浩美, 岩城壮一郎, 藤井聡
  • Journal Title: 心臓 Volume: 42 Pages: 1153-1158
  • NAID: 130002146949
  • Peer Reviewed
• [Journal Article] Increased expression of plasminogen activator inhibitor type-1(PAI-1) in HepG2 cells induced by insulin mediated by the 3'-untranslated region of the PAI-1 gene and its pharmacologic implications (2010)
  • Author(s): Ryu Miyagawa, Takefumi Asakura, Tomomi Nakamura, Hiromi Okada, Soichiro Iwaki, Burton E. Sobel, Satoshi Fujii
  • Journal Title: Coron. Artery Dis. Volume: 21 Pages: 144-150
  • Peer Reviewed
• [Journal Article] HepG2細胞におけるインスリンと酸化ストレスのPAI-1産生に対する影響の解析:メタボリックシンドロームの易血栓性の病態解明を指向して (2010)
  • Author(s): 宮川隆
  • Journal Title: 心臓2010年9月号 Volume: 42 Pages: 1153-1158
  • Peer Reviewed
• [Journal Article] Increased expression of plasminogen activator inhibitor type-1 (PAI-1) in HepG2 cells induced by insulin mediated by the 3'-untranslated region of the PAI-1 gene and its pharmacologic implications (2010)
  • Author(s): Ryu Miyagawa
  • Journal Title: Coronary Artery Disease 21(印刷中)
  • Peer Reviewed
• [Presentation] HepG2細胞におけるS1PによるPAI-1転写後調節機構の解析 (2012)
  • Author(s): 山村周平, 岩城壮一郎, 真川明将, 朝倉健文, 藤井聡
  • Organizer: 日本薬学会第132回年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2012-03-29
• [Presentation] S1PによるPAI-1発現調節機構に関する3'-UTR機能の解析 (2011)
  • Author(s): 山村周平, 真川明将, 朝倉健文, 岩城壮一郎, 藤井聡
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] スフィンゴシン誘導体による虚血再灌流障害抑制作用の解析 (2011)
  • Author(s): 智谷尊史
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] Effect of insulin on the production of plasminogen activator inhibitor type-1 in HepG2 cells : induction through 3'-untranslated region (2011)
  • Author(s): Satoshi Fujii
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis The 57th SSC Meeting
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-07-25
• [Presentation] S1PによるPAI-1発現調節に関わる3'-UTR機能の解析 (2011)
  • Author(s): 山村周平
  • Organizer: 第57回日本薬学会東海支部総会大会
  • Place of Presentation: 金城学院大学
  • Year and Date: 2011-07-11
• [Presentation] スフィンゴシン1-リン酸はEPC様接着細胞におけるeNOSの発現量を増加させる (2011)
  • Author(s): 伊藤史織
  • Organizer: 第12回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2011-06-17
• [Presentation] HepG2細胞におけるcAMPによるPAI-1転写後調節機構の解明 (2011)
  • Author(s): 朝倉健文, 山村周平, 乘本裕明, 岡田浩美, 岩城壮一郎, 藤井聡
  • Organizer: 日本薬学会第131回年会
  • Place of Presentation: ツインメッセ静岡
  • Year and Date: 2011-03-30
• [Presentation] TGF-β誘導性のPAI-1産生と酸化ストレス産生のsimvastatinによる抑制 (2010)
  • Author(s): 中山直美
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] 低酸素とスフィンゴ脂質は脂肪細胞PAI-1発現上昇を通じて肥満者の線溶不全に関与する (2010)
  • Author(s): 小池慶子、大垣恵理華、岩城壮一郎、大川龍之介、矢冨裕、藤井聡
  • Organizer: 第8回血液・血栓オルビス
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 2010-08-21
• [Presentation] 低酸素とスフィンゴ脂質は脂肪細胞PAI-1発現上昇を通じて肥満者の線溶不全に関与する (2010)
  • Author(s): 小池慶子
  • Organizer: 第8回血液・血栓オルビス
  • Place of Presentation: 東京ドームホテル(東京都)
  • Year and Date: 2010-08-21
• [Presentation] Sphingosine-1-phosphate increases hypoxia-induced up-regulation of plasminogen activator inhibitor-1 (2010)
  • Author(s): Soichiro Iwaki
  • Organizer: 第27回内藤コンファレンス 生体膜ダイナミクスと脂質生物学[I]
  • Place of Presentation: シャトレーゼガトーキングダムサッポロ(北海道)
  • Year and Date: 2010-07-01
• [Presentation] スフィンゴシン1-リン酸はNKT細胞の炎症反応を調節する (2010)
  • Author(s): 伊藤史織
  • Organizer: 日本薬学会第130回年会
  • Place of Presentation: 就実大学(岡山県)
  • Year and Date: 2010-03-28
• [Presentation] Sphingosine-1-phosphate increases hypoxia-induced up-regulation of plasminogen activator inhibitor-1 (2010)
  • Author(s): Soichiro Iwaki, Moyoko Asai, Daisuke Sakakibara, Satoshi Fujii
  • Organizer: 第27回内藤コンファレンス生体膜ダイナミクスと脂質生物学[I]
  • Place of Presentation: シャトレーゼガトーキングダムサッポロ
• [Presentation] HepG2細胞におけるPAI-1発現機構の解析:インスリン、cAMP、シロスタゾールの影響 (2009)
  • Author(s): 朝倉健文、岡田浩美、岩城壮一郎、藤井聡
  • Organizer: 第19回日本病院薬剤師会東海ブロック学術大会・平成21年度日本薬学会東海支部例会
  • Place of Presentation: 四日市市文化会館
  • Year and Date: 2009-11-23
• [Presentation] スフィンゴシン1-リン酸は低酸素下におけるHIF-1αを介したPAI-1発現を増加させる (2009)
  • Author(s): 浅井萌子、榊原大輔、岩城壮一郎、藤井聡
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-23
• [Presentation] スフィンゴシン1-リン酸は低酸素下におけるHIF-1αを介したPAI-1発現を増加させる (2009)
  • Author(s): 浅井萌子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-23
• [Presentation] HepG2細胞におけるPAI-1産生と酸化ストレス産生に対するTGF-βの影響とそのメカニズムの解析 (2009)
  • Author(s): 中村友美
  • Organizer: 第32回日本血栓止血学会学術集会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2009-06-06
• [Remarks]
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/szg/index.html