Novel angiogenic therapy using siRNA-loaded Bubble liposomes with ultrasound exposure
| Project/Area Number |
21790164
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 超音波 / リポソーム / siRNA / 核酸 / デリバリー / 核酸デリバリー |
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| Research Abstract |
Recently, we developed polyethyleneglycol(PEG)-modified liposomes(Bubble liposomes ; BLs) entrapping an ultrasound(US) imaging gas. In this study, we prepared novel siRNA-loaded BLs(si-BLs) using cationic lipids, DOTAP, DSTAP, DSDAP, and DDAB, and examined the usability of those BLs as a carrier and an US contrast agent. We demonstrated that siRNA could be loaded onto BLs and that siRNA-loaded BLs was stable in serum. A specific gene-silencing effect was also achieved by transfection with si-BLs. We also could optimize the lipid component of BLs in terms of the US imaging, the gene transfer efficiency, and toxicity. Furthermore, BLs injected into tail vein could reached to ischemic hindlimb of model mice. These results suggest that this method could be a useful tool for angiogenic therapy.
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Report
(4 results)
Research Products
(26 results)
