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Three-dimensional structural and functional analysis of the Golgi apparatus by new method of scanning electron microscopy

Research Project

Project/Area Number 21790176
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNiigata University

Principal Investigator

KOGA Daisuke  新潟大学, 医歯学系, 助教 (30467071)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsゴルジ装置 / 細胞小器官 / 下垂体前葉 / 走査型電子顕微鏡 / オスミウム浸軟法 / ポリエチレングリコール / 試料作製法 / 走査電子顕微鏡 / 下垂体前葉細胞 / ポリエチレングリコール(PEG)
Research Abstract

A novel high resolution three-dimensional observation method was developed by an effective combination of light microscopic technique and scanning electron microscopic observation. This method was especially useful for investigation of tissues containing various kinds of cell, enabling the classification of anterior pituitary cells which has hitherto been difficult by using scanning electron microscopy. Furthermore, the three-dimensional ultrastructure in relation to its function of each anterior pituitary cell of the Golgi apparatus was precisely analyzed by high-resolution scanning electron microscopy.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (24 results)

All 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (18 results)

  • [Journal Article] A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network2012

    • Author(s)
      Watanabe T, Sakai Y, Koga D, Bochimoto H, Hira Y, Hosaka M, Ushiki T
    • Journal Title

      J. Histochem. and Cytochem

      Volume: (in press)

    • NAID

      120006522770

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A useful method for observing intracellular structures of free and cultured cells by scanning electron microscopy2012

    • Author(s)
      Koga D, Nakajima M, Ushiki T
    • Journal Title

      J.Electron Microsc.

      Volume: 61 Pages: 105-111

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope : its functional implications in relation to the arrangement of the microtubule network2012

    • Author(s)
      Tsuyoshi Watanabe, 他
    • Journal Title

      J.Histochem.and Cytochem.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイム3D走査電子顕微鏡の医学生物応用2010

    • Author(s)
      牛木辰男, 伊東祐博, 伊藤広, 岩田太, 甲賀大輔
    • Journal Title

      顕微鏡

      Volume: 45巻3号

    • NAID

      130007788878

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイム3D走査電子顕微鏡の医生物学応用2010

    • Author(s)
      牛木辰男, 他
    • Journal Title

      顕微鏡

      Volume: 45 Pages: 198-201

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A useful method for observing intracellular structures of free and cultured cells by scanning electron microscopy

    • Author(s)
      Koga D, Nakajima M, Ushiki T
    • Journal Title

      J. Electron Microsc

      Volume: 61 Pages: 105-111

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 光顕免疫染色切片像と超高分解能走査電子顕微鏡の直接対比法による細胞内膜系観察法の開発とその応用2012

    • Author(s)
      甲賀大輔
    • Organizer
      日本解剖学会
    • Place of Presentation
      山梨大学
    • Year and Date
      2012-03-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 光顕免疫染色切片像と超高分解能走査電子顕微鏡の直接対比法による細胞内膜系観察法の開発とその応用2012

    • Author(s)
      甲賀大輔
    • Organizer
      日本解剖学会
    • Place of Presentation
      山梨大学(山梨県)
    • Year and Date
      2012-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] The classification of cell types in the pituitary gland by high-resolution stereoscopic scanning electron microscopy2012

    • Author(s)
      甲賀大輔
    • Organizer
      XXI International Symposium on Morphological Sciences
    • Place of Presentation
      Sao Paulo, Brazil
    • Year and Date
      2012-02-15
    • Related Report
      2010 Final Research Report
  • [Presentation] The classification of cell types in the pituitary gland by high-resolution stereoscopic scanning electron microscopy2012

    • Author(s)
      甲賀大輔
    • Organizer
      XXII International Symposium on Morphological Sciences
    • Place of Presentation
      Sao Paulo, Brazil
    • Year and Date
      2012-02-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 培養細胞の細胞内立体微細観察に適した走査電顕オスミウム浸軟法の開発2011

    • Author(s)
      甲賀大輔
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2011-05-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 培養細胞の細胞内立体微細観察に適した走査電顕オスミウム浸軟法の開発2011

    • Author(s)
      甲賀大輔
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2011-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Changes in the ultrastructure of membranous cell organelles during mitosis2011

    • Author(s)
      甲賀大輔
    • Organizer
      日本解剖学会
    • Place of Presentation
      横浜(誌上開催)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Morphological change of membranous cell organelles in the gonadotroph of the rat anterior pituitary gland after castration2010

    • Author(s)
      甲賀大輔
    • Organizer
      XXI International Symposium on Morphological Sciences
    • Place of Presentation
      Taormina, Italy
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 去勢による性腺刺激ホルモン産生(GTH)細胞の細胞内膜系の立体形態機能変化2010

    • Author(s)
      甲賀大輔
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 去勢による性腺刺激ホルモン産生(GTH)細胞の細胞内膜系の立体形態機能変化2010

    • Author(s)
      甲賀大輔
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2010-05-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] ゴルジ装置の多様な形態と機能変化2010

    • Author(s)
      甲賀大輔
    • Organizer
      日本解剖学会
    • Place of Presentation
      岩手県民会館
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] ゴルジ装置の多様な形態と機能変化2010

    • Author(s)
      甲賀大輔
    • Organizer
      解剖学会
    • Place of Presentation
      岩手県民会館
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] バイオ研究におけるSEMの活用とその可能性2009

    • Author(s)
      甲賀大輔
    • Organizer
      SCAN TECH
    • Place of Presentation
      日本女子大学
    • Year and Date
      2009-09-04
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオ研究におけるSEMの活用とその可能性2009

    • Author(s)
      甲賀大輔
    • Organizer
      SCAN TECH(日本顕微鏡学会)
    • Place of Presentation
      日本女子大学
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 走査型電子顕微鏡で下垂体前葉細胞の細胞内膜系の立体微細構造を観察する2009

    • Author(s)
      甲賀大輔
    • Organizer
      日本下垂体研究会
    • Place of Presentation
      青森県三沢市
    • Year and Date
      2009-08-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 走査型電子顕微鏡で下垂体前葉細胞の細胞内膜系の立体微細構造を観察する2009

    • Author(s)
      甲賀大輔
    • Organizer
      日本下垂体研究会
    • Place of Presentation
      青森屋
    • Year and Date
      2009-08-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 去勢によるラット下垂体前葉細胞の細胞内膜系の変化2009

    • Author(s)
      甲賀大輔
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2009-05-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 去勢によるラット下垂体GTH細胞の細胞内膜系の変化2009

    • Author(s)
      甲賀大輔
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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