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Three-dimensional analyses of embryonic development in humans using episcopic fluorescence image capture(EFIC)

Research Project

Project/Area Number 21790180
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyoto University

Principal Investigator

YAMADA Shigehito  京都大学, 医学研究科, 准教授 (80432384)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords先天異常学 / 奇形学 / 発生学 / 三次元イメージング / MR顕微鏡 / EFIC法 / ヒト胚子
Research Abstract

Rapid advances in medical imaging are facilitating the clinical assessment of first trimester human embryos at increasingly earlier stages. To obtain data on early human development, we used episcopic fluorescence capture(EFIC) to acquire digital images of human embryos spanning the time of dynamic tissue remodeling and organogenesis(Carnegie stages 13 to 23). Using these imaging datasets, 3D reconstructions were generated for visualizing different anatomical structures and further morphometrical analyses.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (45 results)

All 2012 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (23 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Digitization of clinical and epidemiological data from the Kyoto Collection of Human Embryos2012

    • Author(s)
      Kameda T, Yamada S, Uwabe C, Suganuma N
    • Journal Title

      Congenital Anomlaies

      Volume: 52(1) Issue: 1 Pages: 48-54

    • DOI

      10.1111/j.1741-4520.2011.00349.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic liver morphology and morphometry by magnetic resonance microscopic imaging2012

    • Author(s)
      Hirose A, Nakashima T, Yamada S, Uwabe C, Kose K, Takakuwa T
    • Journal Title

      Anatomical Record

      Volume: 294 Issue: 1 Pages: 51-59

    • DOI

      10.1002/ar.21496

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphometric analysis of the brain vesicles during the human embryonic period by magnetic resonance microscopic imaging2012

    • Author(s)
      Nakashima T, Hirose A, Yamada S, Uwabe C, Kose K, Takakuwa T
    • Journal Title

      Congenital Anomalies

      Volume: 52 Issue: 1 Pages: 55-58

    • DOI

      10.1111/j.1741-4520.2011.00345.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Movement of the external ear in human embryo2012

    • Author(s)
      Kagurasho M, Yamada S, Uwabe C, Kose K, Takakuwa T
    • Journal Title

      Head & Face Medicine

      Volume: 8 Pages: 2-2

    • NAID

      120004754389

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prenatal Findings in Congenital Leukemia : A Case Report2011

    • Author(s)
      Sato Y, Izumi Y, Minegishi K, Komada M, Yamada S, Kakui K, Tatsumi K, Mikami Y, Fujiwara H, Konishi I
    • Journal Title

      Fetal Diagnosis and Therapy

      Volume: 29 Pages: 325-330

    • NAID

      120005601064

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Normal Location of Thumb/Big Toe may be related to Programmed Cell Death (PCD) in the Preaxial Area of Embryonic Limb2011

    • Author(s)
      Kimura S, Yamada S, Naruse I
    • Journal Title

      Anatomical Record

      Volume: 294 Issue: 8 Pages: 1352-59

    • DOI

      10.1002/ar.21434

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental atlas of the early first trimester human embryo2010

    • Author(s)
      Yamada S, Lee ES, Samtani RR, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW
    • Journal Title

      Dev Dyn

      Volume: 239(6) Pages: 1585-95

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] MR顕微鏡とepiscopic fluorescence image capture(EFIC)法を用いたヒト胚子のイメージング-中枢神経系を中心に-2010

    • Author(s)
      塩田浩平、山田重人、土屋真衣子、巨瀬勝美
    • Journal Title

      小児の脳神経

      Volume: 35(1) Pages: 7-10

    • NAID

      10026528584

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Developmental atlas of the early first trimester human embryo.2010

    • Author(s)
      Yamada S, Lee ES, Samtani RR, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW.
    • Journal Title

      Developmental Dynamics

      Volume: 239 Pages: 1585-95

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New region growing segmentation technique for MR images with weak boundaries.2010

    • Author(s)
      Ayman A, Funatomi T, Mihon M, Elnomery Z, Okada T, Togashi K, Sakai T, Yamada S
    • Journal Title

      IEICE Technical Report

      Volume: 110 Pages: 71-76

    • NAID

      110008152318

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylnitrosourea Induces Neural Progenitor Cell Apoptosis and Microcephaly in Mouse Embryos.2010

    • Author(s)
      Komada M, Fujiyama F, Yamada S, Shiota K, Nagao T.
    • Journal Title

      Birth Defects Res B Dev Reprod Toxicol.

      Volume: 89 Pages: 213-222

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dysregulation of the PDGFRA gene causes inflow tract anomalies including TAPVR : integrating evidence from human genetics and model organisms.2010

    • Author(s)
      Bleyl SB, Saijoh Y, Bax NA, Gittenberger-de Groot AC, Wisse LJ, Chapman SC, Hunter J, Shiratori H, Hamada H, Yamada S, Shiota K, Klewer SE, Leppert MF, Schoenwolf GC.
    • Journal Title

      Human Molecular Genetics

      Volume: 19 Pages: 1286-301

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental atlas of the early first trimester human embryo.2010

    • Author(s)
      Yamada S, Lee ES, Samtani RR, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW.
    • Journal Title

      Developmental Dynamics (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MR顕微鏡とepiscopic fluorescence image capture(EFIC)法を用いたヒト胚子のイメージング -中枢神経系を中心に-2010

    • Author(s)
      塩田浩平、山田重人、土屋真衣子、巨瀬勝美
    • Journal Title

      小児の脳神経 35

      Pages: 7-10

    • NAID

      10026528584

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolated Levocardia : Prenatal diagnosis and management.2009

    • Author(s)
      Katsuya S, Yamada S, Ukita M, Nishimura H, Matsumura N, Fukuhara K, Sato Y, Shiota K, Konishi I.
    • Journal Title

      Congenital Anomalies 49

      Pages: 56-60

    • NAID

      10027388054

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prenatal Findings in Congenital Leukemia : A Case Report.

    • Author(s)
      Sato Y, Izumi Y, Minegishi K, Komada M, Yamada S, Kakui K, Tatsumi K, Mikami Y, Fujiwara H, Konishi I.
    • Journal Title

      Fetal diagnosis and therapy.

      Volume: (In press)

    • NAID

      120005601064

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 『ヒト胚子先天異常・画像データベース』の構築と応用2012

    • Author(s)
      山田重人、亀田知美
    • Organizer
      第64回日本産科婦人科学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-04-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of Human Embryo Database using Kyoto Collection2011

    • Author(s)
      山田重人、亀田知美、元木環、舩冨卓哉、美濃導彦、巨瀬勝美、塩田浩平
    • Organizer
      第56回日本人類遺伝学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Embryo Imaging:京都コレクションと三次元イメージング2011

    • Author(s)
      山田重人
    • Organizer
      第28回関西生殖発生毒性フォーラム
    • Place of Presentation
      大阪(招待講演)
    • Year and Date
      2011-10-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 妊婦の疫学データベースを用いた胚子奇形発生に関する要因の検討~先天異常データベースの網羅的解析~2011

    • Author(s)
      亀田知美、白川康一、土屋真衣子、山崎茉莉、上部千賀子、塩田浩平、山田重人
    • Organizer
      第51回日本先天異常学会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト胚子期における脳室の形態計測学的解析2011

    • Author(s)
      白石直樹、中島崇、山田重人、上部千賀子、巨瀬勝美、高桑徹也
    • Organizer
      第51回日本先天異常学会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト胚子期における隣接器官による肝臓の形態形成への影響2011

    • Author(s)
      高桑徹也、廣瀬あゆみ、濱部佑衣、山田重人、上部千賀子、巨瀬勝美
    • Organizer
      第51回日本先天異常学会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction and Application of Human Embryo Database2011

    • Author(s)
      Yamada S, Kameda T, Takahashi M, Funatomi T, Mizuta S, Matsuda Y, Haishi T, Kose K, Matsuda T, Minoh M, Uwabe C, Shiota K
    • Organizer
      Teratology Society 51st Annual Meeting
    • Place of Presentation
      San Diego CA, USA
    • Year and Date
      2011-06-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト胚子期における脳室の形態計測学的解析(Morphometry of cerebral ventricle during human embryonic period)2011

    • Author(s)
      高桑徹也、中島崇、白石直樹、山田重人、上部千賀子、塩田浩平、巨瀬勝美
    • Organizer
      遺伝医学合同学術集会2011
    • Place of Presentation
      京都
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] "京都コレクション"を用いたヒト胚子3次元立体像(Virtual Human Embryo)の作成(Three dimensional human embryo "Virtual Human Embryo" using MR image data from Kyoto collection)2011

    • Author(s)
      高桑徹也、山田重人、上部千賀子、塩田浩平、巨瀬勝美
    • Organizer
      遺伝医学合同学術集会2011
    • Place of Presentation
      京都
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 「妊婦の疫学データベースを用いた胚子奇形発生に関する要因の検討.先天異常データベースの網羅的解析2011

    • Author(s)
      亀田知美、白川康一、土屋真衣子、山崎茉莉、上部千賀子、塩田浩平、山田重人
    • Organizer
      第51回日本先天異常学会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Construction and Application of Human Embryo Database2011

    • Author(s)
      Yamada S, Kameda T, Takahashi M, Funatomi T, Mizuta S, Matsuda Y, Haishi T, Kose K, Matsuda T, Minoh M, Uwabe C, Shiota K
    • Organizer
      Teratology Society 51^<st> Annual Meeting
    • Place of Presentation
      San Diego, California, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Human Embryology~京都コレクションからみる正常と異常2010

    • Author(s)
      山田重人
    • Organizer
      第50回日本先天異常学会学術集会
    • Place of Presentation
      淡路(招待講演)
    • Year and Date
      2010-07-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロイメージングEFICを用いた実験動物における表現型解析2010

    • Author(s)
      土屋真衣子、山田重人、亀田知美、駒田致和、塩田浩平
    • Organizer
      第50回日本先天異常学会学術集会
    • Place of Presentation
      淡路
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 『ヒト先天異常データベース』の構築と応用2010

    • Author(s)
      亀田知美、山田重人、元木環、高橋三貴子、舩冨卓哉、Ayman Asaad Abdel-Rahman Ahmed、美濃導彦、塩田浩平
    • Organizer
      第50回日本先天異常学会学術集会
    • Place of Presentation
      淡路
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト胚子の形態発生に関する三次元データベースを用いた脳発生の解析2010

    • Author(s)
      中島崇、高桑徹也、廣瀬あゆみ、山田重人、上部千賀子、塩田浩平、巨瀬勝美、杉本直三、酒井朋子
    • Organizer
      第50回日本先天異常学会学術集会
    • Place of Presentation
      淡路
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト胚子MR画像からの3次元立体像2010

    • Author(s)
      高桑徹也、植松南海、布村さゆり、上田愛瑠萌、中島杏子、山田重人、上部千賀子、塩田浩平、巨瀬勝美
    • Organizer
      第50回日本先天異常学会学術集会
    • Place of Presentation
      淡路
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト胚の三次元データベースを用いた肝臓の形態解析2010

    • Author(s)
      廣瀬あゆみ、高桑徹也、中島崇、山田重人、上部千賀子、塩田浩平、巨瀬勝美、杉本直三
    • Organizer
      第50回日本先天異常学会学術集会
    • Place of Presentation
      淡路
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Embryo imaging : Episcopic fluorescence image capture (EPIC) for experimental animals2010

    • Author(s)
      Yamada S, Tsuchiya M, Kameda T, Komada M, Shiota K.
    • Organizer
      Teratology Society 50^<th> Annual Meeting.
    • Place of Presentation
      Louisville, Kentucky, USA
    • Year and Date
      2010-06-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 着床前診断を考慮している不育症カップル15組への第三者的立場からの遺伝カウンセリング2010

    • Author(s)
      澤井英明、山田重人
    • Organizer
      第62回日本産科婦人科学会学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] High-resolution imaging of embryos using episcopic fluorescence image capture (EFIC)2010

    • Author(s)
      土屋真衣子、山田重人、塩田浩平
    • Organizer
      第115回日本解剖学会
    • Place of Presentation
      岩手県民会館(盛岡市)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Embryo imaging : Episcopic fluorescence image capture(EFIC) for experimental animals2010

    • Author(s)
      Yamada S, Tsuchiya M, Kameda T, Komada M, Shiota K.
    • Organizer
      Teratology Society 50^<th> Annual Meeting
    • Place of Presentation
      Louisville, Kentucky, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 胚子を用いた微細標本の新しいイメージングEpiscopic fluorescence image capture(EFIC)の開発2009

    • Author(s)
      土屋真衣子、山田重人、塩田浩平
    • Organizer
      第49回日本先天異常学会
    • Place of Presentation
      鹿児島市民文化ホール(鹿児島市)
    • Year and Date
      2009-06-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト胚の形態発生に関する三次元データベースを用いた胎児正常発生定量化の試み2009

    • Author(s)
      高桑徹也、中島崇、廣瀬あゆみ、山田重人、上部千賀子、塩田浩平
    • Organizer
      第49回日本先天異常学会
    • Place of Presentation
      鹿児島市民文化ホール(鹿児島市)
    • Year and Date
      2009-06-25
    • Related Report
      2009 Annual Research Report
  • [Book] The Human Embryo2012

    • Author(s)
      Edited by Yamada S, Takakuwa T
    • Total Pages
      180
    • Publisher
      InTech
    • Related Report
      2011 Final Research Report
  • [Book] Fine Biomedical Imaging Using X-Ray Phase-Sensitive Technique In : Advanced Biomedical Engineering/Book 2(Edited by : Gaetano D. Gargiulo, Co-editor : Alistair McEwan)2011

    • Author(s)
      Yoneyama A, Yamada S, Takeda T
    • Total Pages
      280
    • Publisher
      InTech
    • Related Report
      2011 Final Research Report
  • [Book] Advanced Biomedical Engineering/Book 2(Edited by : Gaetano D.Gargiulo, Coeditor : Alistair McEwan)2011

    • Author(s)
      Yoneyama A, Yamada S, Takeda T
    • Total Pages
      280
    • Publisher
      InTech
    • Related Report
      2011 Annual Research Report
  • [Book] The Human Embryo(Edited by : Yamada S & Takakuwa T)2011

    • Author(s)
      Yamada S, Takakuwa T
    • Total Pages
      180
    • Publisher
      InTech
    • Related Report
      2011 Annual Research Report
  • [Book] ヒト発生の三次元アトラス(塩田浩平編)2011

    • Author(s)
      山田重人
    • Total Pages
      152
    • Publisher
      日本医事新報社
    • Related Report
      2011 Annual Research Report
  • [Remarks] ホームページ等データベースの一部が公開されている。

    • URL

      http://bird.cac.med.kyoto-u.ac.jp

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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