Analysis of the interaction of STB with steroid hormone receptors and their subcellular dynamics

• Project/Area Number: 21790182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Yamaguchi University
• Principal Investigator: FUJINAGA Ryutaro Yamaguchi University, 大学院・医学系研究科, 講師 (30335723)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: HAP1 / ハンチントン病 / ステロイド受容体 / タイムラプス / プロテアソーム / stigmoid body / 核内移行

Research Abstract

STB interacted with androgen receptor (AR) and glucocorticoid receptor (GR) through their ligand-binding domain in the cytoplasm. The ligand-dependent nuclear translocation of the receptors on STB was attenuated by the treatment of a proteasome inhibitor. The results suggested that STB is involved in the regulation of the cytoplasmic modification and nuclear translocation of AR and GR in a ubiquitin-proteasome system.

Research Products

• [Journal Article] NABIUL ISLAM, MIR RUBAYET JAHAN, AKIE YANAI, KEIJI KOKUBU AND KOH SHINODA.Intracellular colocalization of HAP1/STBs with steroid hormone receptors and its enhancement by a proteasome inhibitor. (2011)
  • Author(s): RYUTARO FUJINAGA, YUKIO TAKESHITA, KAZUHIRO YOSHIOKA, HIROYUKI NAKAMURA, SHUHEI SHINODA, MD.
  • Journal Title: Exp Cell Res. in press
  • Peer Reviewed
• [Journal Article] Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. (2011)
  • Author(s): YUKIO TAKESHITA, RYUTARO FUJINAGA, KEIJI KOKUBU, MD.NABIUL ISLAM, MIR RUBAYET JAHAN, AKIE YANAI, AKIRA KAKIZUKA, KOH SHINODA.
  • Journal Title: Neuroreport 22(5) Pages: 232-238
  • Peer Reviewed
• [Journal Article] Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain (2011)
  • Author(s): Takeshita Y., et al
  • Journal Title: Neuroreport Volume: 22 Pages: 232-238
  • Peer Reviewed
• [Journal Article] Wolfram syndrome 1 (Wfs1) mRNA expression in the normal mouse brain during postnatal development. (2009)
  • Author(s): JUNE KAWANO, RYUTARO FUJINAGA, KIWAKO YAMAMOTO-HANADA K, YOSHITOMO OKA, YUKIO TANIZAWA, KOH SHINODA.
  • Journal Title: Neurosci Res 64(2) Pages: 213-230
  • Peer Reviewed
• [Journal Article] Microtubule-dependent formation of the stigmoid body as a cytoplasmic inclusion distinct from pathological aggresomes. (2009)
  • Author(s): RYUTARO FUJINAGA, YUKIO TAKESHITA, KANAKO UOZUMI, AKIE YANAI, KAZUHIRO YOSHIOKA, KEIJI KOKUBU, KOH SHINODA.
  • Journal Title: Histochem Cell Biol. 132(3) Pages: 305-318
  • Peer Reviewed
• [Journal Article] Microtubule-dependent formation of the stigmoid body as a cytoplasmic inclusion distinct from nathological aggresome. (2009)
  • Author(s): 藤永竜太郎
  • Journal Title: Histochem Cell Biol 132 Pages: 305-318
  • Peer Reviewed
• [Journal Article] Wolfram syndrome 1(Wfs1) mRNA expression in the normal mouse brain during postnatal development. (2009)
  • Author(s): 河野純
  • Journal Title: Neurosci Res 64 Pages: 213-230
  • Peer Reviewed
• [Presentation] Relaying function of the STB/HAP1 on nuclear translocation of androgen and glucocorticoid receptors and its proteasome-dependent regulation (2010)
  • Author(s): Ryutaro Fujinaga, Yukio Takeshita, Kazuhiro Yoshioka, Hiroyuki Nakamura, Shuhei Shinoda, Akie Yanai, Keiji Kokubu, Koh Shinoda
  • Organizer: Society for Neuroscience 40th Annual Meeting
  • Place of Presentation: San Diego USA
  • Year and Date: 2010-11-15
• [Presentation] Relaying function of the STB/HAP1 on nuclear translocation of androgen and glucocorticoid receptors and its proteasome-dependent regulation (2010)
  • Author(s): Ryutaro Fujinaga
  • Organizer: Society for Neuroscience 40th Annual Meeting
  • Place of Presentation: San Diego, USA, San Diego Convention Center
  • Year and Date: 2010-11-15
• [Presentation] STB/HAP1のアンドロゲン及びグルココルチコイドレセプターの核内移行中継機能とプロテアソーム活性の関与 (2010)
  • Author(s): 藤永 竜太郎, 竹下 幸男, 吉岡 和博, 中村 裕幸, 篠田 崇平, 柳井 章江, 国分 啓司, 篠田 晃
  • Organizer: Neuro2010(第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会合同大会)
  • Place of Presentation: 神戸 神戸コンベンションセンター
  • Year and Date: 2010-09-04
• [Presentation] STB/HAP1のアンドロゲン及びグルココルチコイドレセプターの核内移行中継機能とプロテアソーム活性の関与 (2010)
  • Author(s): 藤永竜太郎
  • Organizer: Neuro2010(第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会合同大会)
  • Place of Presentation: 神戸、神戸コンベンションセンター
  • Year and Date: 2010-09-04
• [Presentation] 細胞質内封入体STBとステロイド受容体の相互作用及びその細胞内動態制御 (2010)
  • Author(s): 藤永竜太郎、竹下幸男、吉岡和博、中村裕幸、篠田崇平、柳井章江、國分啓司、篠田晃
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡 岩手県民会館
  • Year and Date: 2010-03-28
• [Presentation] 細胞質内封入体STBとステロイド受容体の相互作用及びその細胞内動態制御 (2010)
  • Author(s): 藤永竜太郎
  • Organizer: 第115回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館、岩手県/盛岡市
  • Year and Date: 2010-03-28
• [Presentation] ラット脳内におけるHAP1の時空間的発現パターンとstigmoid body形成に関する形態学的解析 (2009)
  • Author(s): 藤永竜太郎、魚住加奈子、竹下幸男、柳井章江、國分啓司、松尾義久、篠田晃
  • Organizer: 第50回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 大津 滋賀県立県民交流センター
  • Year and Date: 2009-09-27
• [Presentation] ラット脳内におけるHAP1の時空間的発現パターンとstigmoid body形成に関する形態学的解析 (2009)
  • Author(s): 藤永竜太郎
  • Organizer: 第50回 日本組織細胞化学会総会・学術集会
  • Place of Presentation: ピアザ淡海 滋賀県立県民交流センター、滋賀県/大津市
  • Year and Date: 2009-09-27