Physiological function of ER stress transducer BBF2H7 in chondrogenesis.

• Project/Area Number: 21790184
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Miyazaki
• Principal Investigator: HINO Shinichiro University of Miyazaki, 医学部, 助教 (00372699)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞分化 / 組織形成 / 細胞・組織 / 発生・分化 / 蛋白質 / シグナル伝達

Research Abstract

To assess the function of ER stress transducer BBF2H7 in vivo, we generated BBF2H7 deficient mice. The mice exhibited severe chondrodysplasia. We identified Sec23a, a coat protein complex II component responsible for protein transport from the ER to the Golgi, as a target of BBF2H7. When Sec23a was introduced to BBF2H7 deficient chondrocytes, the impaired transport and secretion of cartilage matrix proteins were totally restored, indicating that the BBF2H7-Sec23a pathway plays crucial roles in chondrogenesis by the activation of protein secretion.

Research Products

• [Journal Article] Distinct mechanisms are responsible for osteopenia and growth retardation in OASIS-deficient mice. (2011)
  • Author(s): Murakami T, Hino S-I, Nishimura R, Yoneda T, Wanaka A, Imaizumi K.
  • Journal Title: Bone 48(3) Pages: 514-523
• [Journal Article] Distinct mechanisms are responsible for osteopenia and growth retardation in OASIS-deficient mice (2011)
  • Author(s): Murakami T
  • Journal Title: Bone Volume: 48 Pages: 512-523
  • Peer Reviewed
• [Journal Article] Effects of the bisphosphonate risedronate on osteopenia in OASIS-deficient mice. (2010)
  • Author(s): Sekiya H, Murakami T, Saito A, Hino S-I, Tsumagari K, Ochiai K, Imaizumi K.
  • Journal Title: Journal of Bone and Mineral Metabolism 28(4) Pages: 384-394
  • NAID: 10027208068
• [Journal Article] Regulation of ER molecular chaperone prevents bone loss in a murine model for osteoporosis. (2010)
  • Author(s): Hino S-I, Kondo S, Yoshinaga K, Saito A, Murakami T, Kanemoto S, Sekiya H, Chihara K, Aikawa Y, Hara H, Kudo T, Sekimoto T, Funamoto T, Chosa E, Imaizumi K.
  • Journal Title: Journal of Bone and Mineral Metabolism 28(2) Pages: 131-138
  • NAID: 10027207004
• [Journal Article] Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation. (2009)
  • Author(s): Murakami T, Saito A, Hino S-I, Kondo S, Kanemoto S, Chihara K, Sekiya H, Tsumagari K, Ochiai K, Yoshinaga K, Saitoh M, Nishimura R, Yoneda T, Kou I, Furuichi T, Ikegawa S, Ikawa M, Okabe M, Wanaka A, Imaizumi K.
  • Journal Title: Nature Cell Biology 11(10) Pages: 1205-1211
• [Journal Article] Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. (2009)
  • Author(s): Saito A, Hino S-I, Murakami T, Kanemoto S, Kondo S, Saitoh M, Nishimura R, Yoneda T, Furuichi T, Ikegawa S, Ikawa M, Okabe M, Imaizumi K(The first two authors are contributed equally to this work)
  • Journal Title: Nature Cell Biology 11(10) Pages: 1197-1204
• [Journal Article] Increased vulnerability of hippocampal pyramidal neurons to the toxicity of kainic acid in OASIS-deficient mice. (2009)
  • Author(s): Chihara K, Saito A, Murakami T, Hino S-I, Aoki Y, Sekiya H, Aikawa Y, Wanaka A, Imaizumi K.
  • Journal Title: Journal of Neurochemistry 110(3) Pages: 956-965
• [Journal Article] Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. (2009)
  • Author(s): Saito A, Hino S-I., et al. (The first two authors are contributed equally to this manuscript)
  • Journal Title: Nature Cell Biology 11 Pages: 1197-1204
  • Peer Reviewed
• [Presentation] 小胞体ストレスセンサーOASISファミリーの生体内機能と活性化制御機能 (2010)
  • Author(s): 日野真一郎、今泉和則
  • Organizer: 日本解剖学会第66回九州支部学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-09
• [Presentation] 小胞体ストレスセンサーOASISファミリーの活性化機構と生体機能調節 (2010)
  • Author(s): 日野真一郎、齋藤敦、村上智彦、津曲健志、落合希実子、今泉和則
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] The roles of ER stress transducer BBF2H7 in chondrogenesis. (2009)
  • Author(s): 日野真一郎
  • Organizer: The American Society for Bone and Mineral Research (ASBMR) 31st Annual Meeting
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 2009-09-11
• [Presentation] Regulation of ER stress response by BBF2H7/Sec23a pathway is essential for chondrogenesis. (2009)
  • Author(s): 齋藤敦, 日野真一郎, 西村理行, 米田俊之,古市達哉, 池川志郎, 今泉和則
  • Organizer: The 26th Naito Conference on Osteobiology.
  • Place of Presentation: Awaji.
• [Presentation] 軟骨形成における小胞体ストレス応答の役割 (2009)
  • Author(s): 津曲健志、齋藤敦、日野真一郎、西村理行、米田俊之、古市達哉、池川志郎、今泉和則
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] The roles of ER stress transducer BBF2H7 in chondrogenesis. (2009)
  • Author(s): 日野真一郎, 齋藤敦, 西村理行, 米田俊之, 池川志郎, 今泉和則
  • Organizer: The American Society for Bone and Mineral Research(ASBMR)31st Annual Meeting.
  • Place of Presentation: Denver, Colorado, USA.
• [Presentation] 小胞体ストレスセンサーBBF2H7の生体内機能 (2009)
  • Author(s): 津曲健志、齋藤敦、日野真一郎、西村理行、米田俊之、古市達哉、池川志郎、今泉和則
  • Organizer: 第33回蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Place of Presentation: 唐津
• [Presentation] 小胞体ストレス応答と軟骨形成 (2009)
  • Author(s): 齋藤敦、日野真一郎、西村理行、米田俊之、古市達哉、今泉和則
  • Organizer: 第27回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 軟骨形成における小胞体ストレスセンサーBBF2H7の役割 (2009)
  • Author(s): 齋藤敦、日野真一郎、西村理行、米田俊之、古市達哉、池川志郎、今泉和則
  • Organizer: 日本分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
• [Remarks] ホームページ等
  • URL: http://www.med.miyazaki-u.ac.jp/anatomy1/
• [Patent(Industrial Property Rights)] 軟骨疾患のモデル非ヒト動物 (2009)
  • Inventor(s): 今泉和則、日野真一郎
  • Industrial Property Rights Holder: 今泉和則、日野真一郎
  • Patent Publication Number: 2009-131169
  • Acquisition Date: 2009-06-18