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Study of the molecular compartment of primary cilia

Research Project

Project/Area Number 21790187
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

SHIBA Dai  Kyoto Prefectural University of Medicine, 医学研究科, 講師 (50360722)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsシグナル伝達 / 細胞・組織 / 一次繊毛 / 遺伝子
Research Abstract

Primary cilia have diverse functions in cell signaling pathways, including mechanosensation and hedgehog signaling. More than 650 proteins are now identified to localize in the cilium. Inv, which is a mouse homolog of human Nphp2, is responsible for situs inversus and multiple renal cysts of the inv mutant mouse. Inv is localized at a distinctive proximal segment of the primary cilium, which we have named as the Inv compartment of the cilium. Nphp3 and Nek8/Nphp9 are also localized to the Inv compartment in wild type cells. Interestingly, the compartment localization of Nphp3 and Nek8/Nphp9 are lost in Inv mutant cells. Introduction of an Inv gene into Inv mutant cells restores in the compartment localization, suggesting that the compartment localization of Nphp3 and Nek8/Nphp9 is dependent on Inv function.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (33 results)

All 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (19 results) Remarks (9 results)

  • [Journal Article] Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of the primary cilia.2010

    • Author(s)
      Shiba D, Manning DK, Koga H, Beier DR, Yokoyama T.
    • Journal Title

      Cytoskeleton 67(2)

      Pages: 112-119

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of the primary cilia.2010

    • Author(s)
      Shiba D, Manning DK, Koga H, Beier DR, Yokoyama T.
    • Journal Title

      Cytoskeleton (Hoboken) 67(2)

      Pages: 112-119

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 嚢胞性腎疾患と繊毛2009

    • Author(s)
      横山尚彦、杉山紀之、芝大
    • Journal Title

      細胞工学 Vol.28, No.10

      Pages: 1021-1026

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Journal Article] Polycystic kidney disease in the medaka (Oryzias latipes) pc mutant caused by a mutation in the Gli-Similar3 (glis3) gene.2009

    • Author(s)
      Hashimoto H, Miyamoto R, Watanabe N, Shiba D, Ozato K, Inoue C, Kubo Y, Koga A, Jindo T, Narita T, Naruse K, Ohishi K, Nogata K, Shin-I T,Asakawa S, Shimizu N, Miyamoto T, MochizukiT, Yokoyama T, Hori H, Takeda H, Kohara Y,Wakamatsu Y.
    • Journal Title

      PLoS One. 4(7)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A murine model of neonatal diabetes mellitus in Glis3-deficient mice.2009

    • Author(s)
      Watanabe N, Hiramatsu K, Miyamoto R, Yasuda K, Suzuki N, Oshima N, Kiyonari H, Shiba D, Ni shio S, Mochizuki T, Yokoyama T, Maruyama S, Matsuo S, Wakamatsu Y, Hashimoto H.
    • Journal Title

      FEBS Lett. 583(12)

      Pages: 2108-2113

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Renal cystic diseases as a cilliopathy2011

    • Author(s)
      Takahiko Yokoyama, Noriyuki Sugiyama, Dai Shiba, Hajime Fukui, Kana Nakata
    • Organizer
      第116回解剖学会全国学術集会抄録 The Journal of Physiological Sciences 61(suppl) : s78(2011)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Searching for the region regulating ciliary localization of Glis32011

    • Author(s)
      Miki Miyamoto, Dai Shiba, Hisashi Hashimoto, Takahiko Yokoyama
    • Organizer
      第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences61(suppl) : s104(2011)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional analysis of ciliary protein Nek8 in zebrafish2011

    • Author(s)
      Ryousuke Ikeda, Hajime Fukui, Dai Shiba, Takahiko Yokoyama
    • Organizer
      第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences61(suppl) : s104(2011)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Ciliary localization of Inv is essential for in vivo function and is regulated by its IQ domain2011

    • Author(s)
      Hajime Fukui, Dai Shiba, Takahiko Yokoyama
    • Organizer
      第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences61(suppl) : s274 (2011)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] NPHP pathway in the primary cilia2011

    • Author(s)
      Dai Shiba, Takahiko Yokoyama
    • Organizer
      第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences 61(suppl) : s275 (2011)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] NPHP pathway in the primary cilia2011

    • Author(s)
      Shiba D, Yokoyama T
    • Organizer
      第116回 解剖学会全国学術集会
    • Place of Presentation
      (震災のため誌上発表 The Journal of Physiological Sciences 61(suppl) : s275(2011))
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ciliary localization of Lnv is essential for in vivo function and is regulated by its IQ dimain2011

    • Author(s)
      Fukui H, Shiba D, Yokoyama T
    • Organizer
      第116回 解剖学会全国学術集会
    • Place of Presentation
      (震災のため誌上発表 The Journal of Physiological Sciences 61(suppl) : s274(2011))
    • Related Report
      2010 Annual Research Report
  • [Presentation] 嚢胞性腎疾患と一次繊毛機能2010

    • Author(s)
      芝大
    • Organizer
      大塚製薬株式会社 学術講演会
    • Place of Presentation
      大塚製薬株式会社 徳島研究所
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 一次繊毛内分子領域「Invコンパートメント」へのNphp3・Nek8集積機構解析2010

    • Author(s)
      芝大、萩原治夫、横山尚彦
    • Organizer
      第115回解剖学会全国学術集会
    • Place of Presentation
      岩手県民会館(岩手)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Localization and relationship among NPHP proteins in the primary cilia2010

    • Author(s)
      Dai Shiba, Danielle K.Manning, David R.Beier, Takahiko Yokoyama
    • Organizer
      The American Society for Cell Biology 50th Annual Meeting抄録 The American Society for Cell Biology, Annual Meeting Program, 182 (2010)
    • Place of Presentation
      Pennsylvania Convention Center, Philadelphia, PA
    • Related Report
      2010 Final Research Report
  • [Presentation] 一次繊毛内分子領域「Invコンパートメント」へのNphp3・Nek8集積機構解析Inv-based molecular accumulation machinery in the proximal segment of primary cilium2010

    • Author(s)
      芝大、萩原治夫、横山尚彦
    • Organizer
      第115回解剖学会全国学術集会抄録 解剖学雑誌,85 (Suppl) : 108(2010)
    • Place of Presentation
      岩手
    • Related Report
      2010 Final Research Report
  • [Presentation] Inv蛋白におけるIQドメイン機能解析福井一2010

    • Author(s)
      芝大、横山尚彦
    • Organizer
      第115回解剖学会全国学術集会抄録 解剖学雑誌,85 (Suppl) : 136(2010)
    • Place of Presentation
      岩手
    • Related Report
      2010 Final Research Report
  • [Presentation] 若年性ネフロン癆責任遺伝子産物 Nphp3の繊毛局在制御ドメイン解析Analysis of cilium localization signal of Nphp32010

    • Author(s)
      中田香奈、芝大、横山尚彦
    • Organizer
      第115回解剖学会全国学術集会抄録 解剖学雑誌,85 (Suppl) 108 (2010)
    • Place of Presentation
      岩手
    • Related Report
      2010 Final Research Report
  • [Presentation] Localization and relationship among NPHP proteins in the primary cilia2010

    • Author(s)
      Shiba D, Manning DK, Beier DR, Yokoyama T
    • Organizer
      The American Society for Cell Biology 50th Annual Meeting
    • Place of Presentation
      Pennsylvania Convention Center, PA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Inv compartment of the primary cilia2009

    • Author(s)
      芝大、古閑比佐志、横山尚彦.
    • Organizer
      第61回日本細胞生物学会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Inv compartment of the primary cilia2009

    • Author(s)
      Yokoyama T, Shiba D
    • Organizer
      Society for Developmental Biology 68th Annual Meeting抄録 Developmental Biology, 331, A125(2009)
    • Place of Presentation
      Hyatt Regency, San Francisco, CA
    • Related Report
      2010 Final Research Report
  • [Presentation] 尿細管形態維持装置としての繊毛蛋白Inv の機能Cilia as a maintainer for renal tubule diameter-Functional analysis of ciliary protein Inv-2009

    • Author(s)
      横山尚彦、杉山紀之、芝大
    • Organizer
      第32回分子生物学会年会,抄録MBSJ program 2009, 99 (2009)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Inv controls the intra-ciliary molecular compartmentalization of renal epithelial primary cilia2009

    • Author(s)
      芝大、古閑比佐志、横山尚彦
    • Organizer
      第61回日本細胞生物学会抄録 第61回日本細胞生物学会 要旨集,224(2009)
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] Inv controls the intra-ciliazy moleculaz compartmentalization of renal epithelial primary cilia2009

    • Author(s)
      Yokoyama T, Shiba D
    • Organizer
      Society for Developmental Biology 68th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2009 Annual Research Report
  • [Remarks] その他活動

    • Related Report
      2010 Final Research Report
  • [Remarks] 繊毛研究会の立ち上げ

    • Related Report
      2010 Final Research Report
  • [Remarks] 国内で繊毛研究を行っている研究者は多数いるが、その情報交換を効率的に行うために、繊毛研究会を立ち上げた。数名の研究メンバーとともに会の発足に貢献した

    • Related Report
      2010 Final Research Report
  • [Remarks] 第一回研究会を2010年11月13日「繊毛の構造と機能のインターフェイス」と題し、基礎生物学研究所(明大寺地区)会議室(111号室)で行った

    • URL

      http://www.geocities.jp/cilia_club/

    • Related Report
      2010 Final Research Report
  • [Remarks] 学術講演会

    • Related Report
      2010 Final Research Report
  • [Remarks] 2010年11月16日「嚢胞性腎疾患と一次繊毛機能」と題し、大塚製薬徳島研究所(徳島) で学術講演会を行った。

    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.f.kpu-m.ac.jp/k/anat2/Projects/thema2.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.f.kpu-m.ac.jp/k/anat2/Projects/thema2.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.f.kpu-m.ac.jp/k/anat2/Projects/thema2.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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