Study of the molecular compartment of primary cilia

• Project/Area Number: 21790187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SHIBA Dai Kyoto Prefectural University of Medicine, 医学研究科, 講師 (50360722)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: シグナル伝達 / 細胞・組織 / 一次繊毛 / 遺伝子

Research Abstract

Primary cilia have diverse functions in cell signaling pathways, including mechanosensation and hedgehog signaling. More than 650 proteins are now identified to localize in the cilium. Inv, which is a mouse homolog of human Nphp2, is responsible for situs inversus and multiple renal cysts of the inv mutant mouse. Inv is localized at a distinctive proximal segment of the primary cilium, which we have named as the Inv compartment of the cilium. Nphp3 and Nek8/Nphp9 are also localized to the Inv compartment in wild type cells. Interestingly, the compartment localization of Nphp3 and Nek8/Nphp9 are lost in Inv mutant cells. Introduction of an Inv gene into Inv mutant cells restores in the compartment localization, suggesting that the compartment localization of Nphp3 and Nek8/Nphp9 is dependent on Inv function.

Research Products

• [Journal Article] Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of the primary cilia. (2010)
  • Author(s): Shiba D, Manning DK, Koga H, Beier DR, Yokoyama T.
  • Journal Title: Cytoskeleton 67(2) Pages: 112-119
  • Peer Reviewed
• [Journal Article] Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of the primary cilia. (2010)
  • Author(s): Shiba D, Manning DK, Koga H, Beier DR, Yokoyama T.
  • Journal Title: Cytoskeleton (Hoboken) 67(2) Pages: 112-119
  • Peer Reviewed
• [Journal Article] 嚢胞性腎疾患と繊毛 (2009)
  • Author(s): 横山尚彦、杉山紀之、芝大
  • Journal Title: 細胞工学 Vol.28, No.10 Pages: 1021-1026
• [Journal Article] Polycystic kidney disease in the medaka (Oryzias latipes) pc mutant caused by a mutation in the Gli-Similar3 (glis3) gene. (2009)
  • Author(s): Hashimoto H, Miyamoto R, Watanabe N, Shiba D, Ozato K, Inoue C, Kubo Y, Koga A, Jindo T, Narita T, Naruse K, Ohishi K, Nogata K, Shin-I T,Asakawa S, Shimizu N, Miyamoto T, MochizukiT, Yokoyama T, Hori H, Takeda H, Kohara Y,Wakamatsu Y.
  • Journal Title: PLoS One. 4(7)
  • Peer Reviewed
• [Journal Article] A murine model of neonatal diabetes mellitus in Glis3-deficient mice. (2009)
  • Author(s): Watanabe N, Hiramatsu K, Miyamoto R, Yasuda K, Suzuki N, Oshima N, Kiyonari H, Shiba D, Ni shio S, Mochizuki T, Yokoyama T, Maruyama S, Matsuo S, Wakamatsu Y, Hashimoto H.
  • Journal Title: FEBS Lett. 583(12) Pages: 2108-2113
  • Peer Reviewed
• [Presentation] Renal cystic diseases as a cilliopathy (2011)
  • Author(s): Takahiko Yokoyama, Noriyuki Sugiyama, Dai Shiba, Hajime Fukui, Kana Nakata
  • Organizer: 第116回解剖学会全国学術集会抄録 The Journal of Physiological Sciences 61(suppl) : s78(2011)
  • Place of Presentation: 横浜
• [Presentation] Searching for the region regulating ciliary localization of Glis3 (2011)
  • Author(s): Miki Miyamoto, Dai Shiba, Hisashi Hashimoto, Takahiko Yokoyama
  • Organizer: 第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences61(suppl) : s104(2011)
  • Place of Presentation: 横浜
• [Presentation] Functional analysis of ciliary protein Nek8 in zebrafish (2011)
  • Author(s): Ryousuke Ikeda, Hajime Fukui, Dai Shiba, Takahiko Yokoyama
  • Organizer: 第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences61(suppl) : s104(2011)
  • Place of Presentation: 横浜
• [Presentation] Ciliary localization of Inv is essential for in vivo function and is regulated by its IQ domain (2011)
  • Author(s): Hajime Fukui, Dai Shiba, Takahiko Yokoyama
  • Organizer: 第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences61(suppl) : s274 (2011)
  • Place of Presentation: 横浜
• [Presentation] NPHP pathway in the primary cilia (2011)
  • Author(s): Dai Shiba, Takahiko Yokoyama
  • Organizer: 第116回解剖学会全国学術集会 抄録 The Journal of Physiological Sciences 61(suppl) : s275 (2011)
  • Place of Presentation: 横浜
• [Presentation] NPHP pathway in the primary cilia (2011)
  • Author(s): Shiba D, Yokoyama T
  • Organizer: 第116回 解剖学会全国学術集会
  • Place of Presentation: (震災のため誌上発表 The Journal of Physiological Sciences 61(suppl) : s275(2011))
• [Presentation] Ciliary localization of Lnv is essential for in vivo function and is regulated by its IQ dimain (2011)
  • Author(s): Fukui H, Shiba D, Yokoyama T
  • Organizer: 第116回 解剖学会全国学術集会
  • Place of Presentation: (震災のため誌上発表 The Journal of Physiological Sciences 61(suppl) : s274(2011))
• [Presentation] 嚢胞性腎疾患と一次繊毛機能 (2010)
  • Author(s): 芝大
  • Organizer: 大塚製薬株式会社 学術講演会
  • Place of Presentation: 大塚製薬株式会社 徳島研究所
  • Year and Date: 2010-11-16
• [Presentation] 一次繊毛内分子領域「Invコンパートメント」へのNphp3・Nek8集積機構解析 (2010)
  • Author(s): 芝大、萩原治夫、横山尚彦
  • Organizer: 第115回解剖学会全国学術集会
  • Place of Presentation: 岩手県民会館(岩手)
  • Year and Date: 2010-03-28
• [Presentation] Localization and relationship among NPHP proteins in the primary cilia (2010)
  • Author(s): Dai Shiba, Danielle K.Manning, David R.Beier, Takahiko Yokoyama
  • Organizer: The American Society for Cell Biology 50th Annual Meeting抄録 The American Society for Cell Biology, Annual Meeting Program, 182 (2010)
  • Place of Presentation: Pennsylvania Convention Center, Philadelphia, PA
• [Presentation] 一次繊毛内分子領域「Invコンパートメント」へのNphp3・Nek8集積機構解析Inv-based molecular accumulation machinery in the proximal segment of primary cilium (2010)
  • Author(s): 芝大、萩原治夫、横山尚彦
  • Organizer: 第115回解剖学会全国学術集会抄録 解剖学雑誌,85 (Suppl) : 108(2010)
  • Place of Presentation: 岩手
• [Presentation] Inv蛋白におけるIQドメイン機能解析福井一 (2010)
  • Author(s): 芝大、横山尚彦
  • Organizer: 第115回解剖学会全国学術集会抄録 解剖学雑誌,85 (Suppl) : 136(2010)
  • Place of Presentation: 岩手
• [Presentation] 若年性ネフロン癆責任遺伝子産物 Nphp3の繊毛局在制御ドメイン解析Analysis of cilium localization signal of Nphp3 (2010)
  • Author(s): 中田香奈、芝大、横山尚彦
  • Organizer: 第115回解剖学会全国学術集会抄録 解剖学雑誌,85 (Suppl) 108 (2010)
  • Place of Presentation: 岩手
• [Presentation] Localization and relationship among NPHP proteins in the primary cilia (2010)
  • Author(s): Shiba D, Manning DK, Beier DR, Yokoyama T
  • Organizer: The American Society for Cell Biology 50th Annual Meeting
  • Place of Presentation: Pennsylvania Convention Center, PA, USA
• [Presentation] Inv compartment of the primary cilia (2009)
  • Author(s): 芝大、古閑比佐志、横山尚彦.
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2009-06-03
• [Presentation] Inv compartment of the primary cilia (2009)
  • Author(s): Yokoyama T, Shiba D
  • Organizer: Society for Developmental Biology 68th Annual Meeting抄録 Developmental Biology, 331, A125(2009)
  • Place of Presentation: Hyatt Regency, San Francisco, CA
• [Presentation] 尿細管形態維持装置としての繊毛蛋白Inv の機能Cilia as a maintainer for renal tubule diameter-Functional analysis of ciliary protein Inv- (2009)
  • Author(s): 横山尚彦、杉山紀之、芝大
  • Organizer: 第32回分子生物学会年会,抄録MBSJ program 2009, 99 (2009)
  • Place of Presentation: 横浜
• [Presentation] Inv controls the intra-ciliary molecular compartmentalization of renal epithelial primary cilia (2009)
  • Author(s): 芝大、古閑比佐志、横山尚彦
  • Organizer: 第61回日本細胞生物学会抄録 第61回日本細胞生物学会 要旨集,224(2009)
  • Place of Presentation: 名古屋
• [Presentation] Inv controls the intra-ciliazy moleculaz compartmentalization of renal epithelial primary cilia (2009)
  • Author(s): Yokoyama T, Shiba D
  • Organizer: Society for Developmental Biology 68th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Remarks] その他活動
• [Remarks] 繊毛研究会の立ち上げ
• [Remarks] 国内で繊毛研究を行っている研究者は多数いるが、その情報交換を効率的に行うために、繊毛研究会を立ち上げた。数名の研究メンバーとともに会の発足に貢献した
• [Remarks] 第一回研究会を2010年11月13日「繊毛の構造と機能のインターフェイス」と題し、基礎生物学研究所(明大寺地区)会議室(111号室)で行った
  • URL: http://www.geocities.jp/cilia_club/
• [Remarks] 学術講演会
• [Remarks] 2010年11月16日「嚢胞性腎疾患と一次繊毛機能」と題し、大塚製薬徳島研究所(徳島) で学術講演会を行った。
• [Remarks] ホームページ等
  • URL: http://www.f.kpu-m.ac.jp/k/anat2/Projects/thema2.html
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/anat2/Projects/thema2.html
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/anat2/Projects/thema2.html