Study of the molecular compartment of primary cilia
Project/Area Number |
21790187
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
SHIBA Dai Kyoto Prefectural University of Medicine, 医学研究科, 講師 (50360722)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | シグナル伝達 / 細胞・組織 / 一次繊毛 / 遺伝子 |
Research Abstract |
Primary cilia have diverse functions in cell signaling pathways, including mechanosensation and hedgehog signaling. More than 650 proteins are now identified to localize in the cilium. Inv, which is a mouse homolog of human Nphp2, is responsible for situs inversus and multiple renal cysts of the inv mutant mouse. Inv is localized at a distinctive proximal segment of the primary cilium, which we have named as the Inv compartment of the cilium. Nphp3 and Nek8/Nphp9 are also localized to the Inv compartment in wild type cells. Interestingly, the compartment localization of Nphp3 and Nek8/Nphp9 are lost in Inv mutant cells. Introduction of an Inv gene into Inv mutant cells restores in the compartment localization, suggesting that the compartment localization of Nphp3 and Nek8/Nphp9 is dependent on Inv function.
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Report
(3 results)
Research Products
(33 results)
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[Journal Article] Polycystic kidney disease in the medaka (Oryzias latipes) pc mutant caused by a mutation in the Gli-Similar3 (glis3) gene.2009
Author(s)
Hashimoto H, Miyamoto R, Watanabe N, Shiba D, Ozato K, Inoue C, Kubo Y, Koga A, Jindo T, Narita T, Naruse K, Ohishi K, Nogata K, Shin-I T,Asakawa S, Shimizu N, Miyamoto T, MochizukiT, Yokoyama T, Hori H, Takeda H, Kohara Y,Wakamatsu Y.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A murine model of neonatal diabetes mellitus in Glis3-deficient mice.2009
Author(s)
Watanabe N, Hiramatsu K, Miyamoto R, Yasuda K, Suzuki N, Oshima N, Kiyonari H, Shiba D, Ni shio S, Mochizuki T, Yokoyama T, Maruyama S, Matsuo S, Wakamatsu Y, Hashimoto H.
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Journal Title
FEBS Lett. 583(12)
Pages: 2108-2113
Related Report
Peer Reviewed
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