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Dynamic functional morphology of pheromone signaling-Identification of estrous pheromone and its receptive vomeronasal neurons-

Research Project

Project/Area Number 21790188
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionIwate Medical University

Principal Investigator

AKUTSU Hitomi  Iwate Medical University, 医学部, 助教 (30398482)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsフェロモン / カルシウムイメージング / 鋤鼻器 / 生理活性物質 / バイオアッセイ / ラット / 尿 / カルシウムイオン / イメージング
Research Abstract

We found out in earlier study that female rats' urine excreted on proestrous stage (proestrous urine) had some bioactivity which induced a specific increase of intracellular Ca^<2+> concentration ([Ca^<2+>]_i) in vomeronasal sensory neurons of male rat. By set such specific [Ca^<2+>]_i increase as a indicator of bioactivity, in this study, we aimed to identify a bioactive substance, a pheromone, which was specific contained in proestrous urine with bioassay systems of calcium imaging of vomeronasal sensory neuron (VSN). Female rat's urine sample, treated with ultrafiltration membrane or boiled, were tested bioactivity with the bioassay system. It became clear that urinary substances which seemed to induce a specific [Ca^<2+>]_i increase have properties as a low molecular and hard to denature by heat. By purification of female rats' urine with HPLC using hydrophobic column, most components of urine were eluted in low concentration acetonitril (0-15%). When HPLC fractions were examined its bioactivity by "a bioassay system based on fluorescent microscope" which was built up in this study, bioactivity was observed as a specific [Ca^<2+>]_i increase in some HPLC fractions of proestrous urine. We have applied the bioassay system using real time laser scanning microscope to measure the responses in individual VSNs, the new bioassay system based on fluorescent microscope was developed to measure the responses of the whole vomeronasal sensory epithelium and the mass of VSN. The purification of urinary component and the bioactivity examination by two ways of bioassay raised a possibility to identify a proestrous urine specific pheromone.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2011 2010 Other

All Presentation (7 results) Remarks (1 results)

  • [Presentation] Purification of the bioactive factors in female rats' urine which stimulate male rats' vomeronasal sensory neurons2011

    • Author(s)
      Hitomi Akutsu, Jiro Hitomi, Yoh-ichi Satoh
    • Organizer
      第88回日本生理学会大会 第116会日本解剖学会総会・全国学術集会 合同大会(ポスター発表)
    • Place of Presentation
      震災のため,誌上開催
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Purification of the bioactive factors in female rats' urine which stimulate male rats' vomeronasal sensory neurons2011

    • Author(s)
      Hitomi Akutsu, Jiro Hitomi, Yoh-ichi Satoh
    • Organizer
      第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
    • Place of Presentation
      誌上開催(パシフィコ横浜)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 尿が誘発する鋤鼻感覚細胞内Ca^<2+>の上昇(シンポジウム)2010

    • Author(s)
      阿久津仁美
    • Organizer
      日本農芸化学会東北支部シンポジウム
    • Place of Presentation
      岩手大学・岩手
    • Year and Date
      2010-06-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 尿が誘発する鋤鼻感覚細胞内Ca^<2+>の上昇2010

    • Author(s)
      阿久津仁美, 人見次郎, 佐藤洋一
    • Organizer
      日本農芸化学会東北支部シンポジウム
    • Place of Presentation
      岩手大学
    • Year and Date
      2010-06-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 尿が誘発する鋤鼻感覚細胞内Ca^<2+>上昇パターンの多様性2010

    • Author(s)
      阿久津仁美
    • Organizer
      日本解剖学会第115回総会・全国学術集会
    • Place of Presentation
      岩手県民会館(岩手・盛岡)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 尿が誘発する鋤鼻感覚細胞内Ca^<2+>上昇パターンの多様性(シンポジウム)2010

    • Author(s)
      阿久津仁美, 人見次郎, 佐藤洋一
    • Organizer
      第115回日本解剖学会・全国学術集会
    • Place of Presentation
      岩手県民会館・岩手
    • Related Report
      2010 Final Research Report
  • [Presentation] New era of morphology-Developed by the fluorescent markers and the confocal microscopy.2010

    • Author(s)
      Satoh Y, Saino T, Akutsu-Yamauchi H, Hamano Y
    • Organizer
      XXI International Symposium on Morphological Sciences
    • Place of Presentation
      Taormina, Italy
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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