Functional analysis of LC3A, mammalian ortholog of yeast autophagy-related gene Atg8

• Project/Area Number: 21790192
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Juntendo University
• Principal Investigator: SASAKI Mitsuho Juntendo University, 医学部, 助教 (20432536)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞機能形態学 / オートファジー / ノックアウトマウス / LC3 / ジーントラップ法

Research Abstract

A microtubule-associated protein 1 light chain 3 (MAP1LC3) is an essential factor of autophagosomal membrane formation. The amino acid sequence of LC3A is highly similar to that of LC3B, but the functional property of LC3A is still unknown. To characterize the function of LC3A, we generated LC3A conditional knockout mice.

Research Products

• [Journal Article] LC3, a microtubule-associated protein 1A/B light chain 3, is involved in cytoplasmic lipid droplet formation. (2010)
  • Author(s): Shibata M, Yoshimura K, Tamura H, Ueno T, Nishimura T, Inoue T, Sasaki M, Koike M, Arai H, Kominami E, Uchiyama Y.
  • Journal Title: Biochemical and Biophysical Research Communication vol.393 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains. (2010)
  • Author(s): Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y.
  • Journal Title: Journal of Histochemistry and Cytochemistry vol.58 Pages: 443-453
  • Peer Reviewed
• [Journal Article] Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains. (2010)
  • Author(s): Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y.
  • Journal Title: J.Histochem.Cytochem. Volume: 58 Pages: 443-453
  • Peer Reviewed
• [Journal Article] LC3, a microtubule-associated protein 1A/B light chain 3, is involved in cytoplasmic lipid droplet formation. (2010)
  • Author(s): Shibata M, Yoshimura K, Tamura H, Ueno T, Nishimura T, Inoue T, Sasaki M, Koike M, Arai H, Kominami E, Uchiyama Y.
  • Journal Title: Bichem.Biophys.Res.Commun. 393 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains.
  • Author(s): Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y.
  • Journal Title: J.Histochem.Cytochem. (in press)
  • Peer Reviewed
• [Presentation] 哺乳類Atg9はオメガソームに局在する (2011)
  • Author(s): 佐々木光穂、多村博澄、内山安男
  • Organizer: 第116回 日本解剖学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-03-30
• [Presentation] 哺乳類Atg9A mRNAノックダウン細胞株におけるリソソーム様構造物の生化学的解析 (2010)
  • Author(s): 佐々木光穂、多村博澄、小池正人、上野隆、内山安男
  • Organizer: 第115回 日本解剖学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-30
• [Presentation]
  • Organizer: 第115回日本解剖学会総会
• [Presentation]
  • Organizer: 第116回日本解剖学会総会
• [Remarks] ホームページ等
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/shinkei_kozo/index.html