Study on the molecular mechanism of hormone secretion

• Project/Area Number: 21790197
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: The University of Tokyo
• Principal Investigator: TSUBOI Takashi The University of Tokyo, 大学院・総合文化研究科, 准教授 (80415231)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 開口放出 / ミオシン2 / マイクロRNA / ホルモン / 神経内分泌細胞 / バイオイメージング / 細胞骨格 / 分泌顆粒 / 可視化 / 生体分子 / 生理学 / 蛋白質 / 神経・脳

Research Abstract

To adapt environmental changes, endocrine tissues in our body are secreted variety of hormones to maintain body homeostasis. Failure of this mechanism is directly linked to various disease such as diabetes and allergies. In the present study, we identified an myosin 2 as a regulator for hormone secretion by using biochemical, physiological, and bioimaging analyses.

Research Products

• [Journal Article] Dual roles of Notch in regulation of apically restricted mitosis and apicobasal polarity of neuroepithelial cells. (2011)
  • Author(s): Ohata S, Aoki R, Kinoshita S, Yamaguchi M, Tsuruoka-Kinoshita S, Tanaka H, Wada H, Watabe S, Tsuboi T, Masai I, Okamoto H.
  • Journal Title: Neuron 69 Pages: 215-230
  • Peer Reviewed
• [Journal Article] Dual roles of Notch in regulation of apically restricted mitosis and apicobasal polarity of neuroepithelial cells (2011)
  • Author(s): Ohata S, Aoki R, Kinoshita S, Yamaguchi M, Tsuruoka-Kinoshita S, Tanaka H, Wada H, Watabe S, Tsuboi T, Masai I, Okamoto H
  • Journal Title: Neuron Volume: 69 Pages: 215-230
  • Peer Reviewed
• [Journal Article] Myosin II modulates fusion pore opening period via cortical F-actin reorganization (2010)
  • Author(s): Aoki R, Kitaguchi T, Miyawaki A, Tsuboi T.
  • Journal Title: The Journal of Physiological Sciences 60 Pages: 176-176
• [Journal Article] Molecular mechanism of glutamate-induced insulin secretion from pancreatic b-cells revealed by genetically encoded fluorescent indicators (2010)
  • Author(s): Oya M, Sato M, Suzuki H, Sato M, Tsuboi T.
  • Journal Title: The Journal of Physiological Sciences 60 Pages: 175-175
• [Journal Article] Miyawaki A Age-dependent preferential secretory vesicle exocytosis in neuroendocrine cells (2010)
  • Author(s): Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M
  • Journal Title: The Journal of Physiological Sciences 60 Pages: 11-11
• [Journal Article] Golgi-associated GSK3-beta regulates the sorting process of post-Golgi membrane trafficking. (2010)
  • Author(s): Adachi A, Kano F, Tsuboi T, Fujita M, Maeda Y, Murata M.
  • Journal Title: Journal of Cell Sciences 123 Pages: 3215-3225
  • Peer Reviewed
• [Journal Article] Effect of glucocorticoid on the biosynthesis of growth hormone-containing secretory granules in pituitary cells. (2010)
  • Author(s): Kawashima R, Ikematsu K, Abe Y, Sato M, Tsuruya S, Nakasono I, Fukushima H, Inoue K, Tsuboi T.
  • Journal Title: Biochemical and Biophysical Research Communications 400 Pages: 225-229
  • Peer Reviewed
• [Journal Article] Development of Luciferin Analogues Bearing an Amino Group and Their Application as BRET Donors Chemistry-An (2010)
  • Author(s): Takakura H, Sasakura K, Ueno T, Urano Y, Terai T, Hanaoka K, Tsuboi T, Nagano T.
  • Journal Title: Asian Journal 5 Pages: 2053-2061
  • Peer Reviewed
• [Journal Article] Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis (2010)
  • Author(s): Aoki R, Kitaguchi T, Oya M, Yanagihara Y, Sato M, Miyawaki A, Tsuboi T.
  • Journal Title: Biochemical Journal 429 Pages: 497-504
  • Peer Reviewed
• [Journal Article] Role of the polybasic sequence in the Doc2alpha C2B domain in dense-core vesicle exocytosis in PC12 cells. (2010)
  • Author(s): Sato M, Mori Y, Matsui T, Aoki R, Oya M, Yanagihara Y, Fukuda M, Tsuboi T.
  • Journal Title: Journal of Neurochemistry 114 Pages: 171-181
  • Peer Reviewed
• [Journal Article] Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. (2010)
  • Author(s): Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A.
  • Journal Title: Molecular Biology of the Cell 21 Pages: 87-94
  • Peer Reviewed
• [Journal Article] Role of the polybasic sequence in the Doc2alpha C2B domain in dense-core vesicle exocytosis in PC12 cells (2010)
  • Author(s): Sato M, Mori Y, Matsui T, Aoki R, Oya M, Yanagihara Y, Fukuda M, Tsuboi T
  • Journal Title: Journal of Neurochemistry Volume: 114 Pages: 171-181
  • Peer Reviewed
• [Journal Article] Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis (2010)
  • Author(s): Aoki R, Kitaguchi T, Oya M, Yanagihara Y, Sato M, Miyawaki A, Tsuboi T
  • Journal Title: Biochemical Journal Volume: 429 Pages: 497-504
  • Peer Reviewed
• [Journal Article] Development of Luciferin Analogues Bearing an Amino Group and Their Application as BRET Donors (2010)
  • Author(s): Takakura H, Sasakura K, Ueno T, Urano Y, Terai T, Hanaoka K, Tsuboi T, Nagano T
  • Journal Title: Chemistry-An Asian Journal Volume: 5 Pages: 2053-2061
  • Peer Reviewed
• [Journal Article] Golgi-associated GSK3-beta regulates the sorting process of post-Golgi membrane trafficking (2010)
  • Author(s): Adachi A, Kano F, Tsuboi T, Fujita M, Maeda Y, Murata M
  • Journal Title: Journal of Cell Science Volume: 123 Pages: 3215-3225
  • Peer Reviewed
• [Journal Article] Effect of glucocorticoid on the biosynthesis of growth hormone-containing secretory granules in pituitary cells (2010)
  • Author(s): Kawashima R, Ikematsu K, Abe Y, Sato M, Tsuruya S, Nakasono I, Fukushima H, Inoue K, Tsuboi T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 400 Pages: 225-229
  • Peer Reviewed
• [Journal Article] Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein (2010)
  • Author(s): Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A.
  • Journal Title: Molecular Biology of the Cell 21 Pages: 87-94
  • Peer Reviewed
• [Journal Article] Myosin II regulates "kiss-and-run" dense-core exocytosis revealed by total internal reflection fluorescence microscopy. (2009)
  • Author(s): Aoki R, Kitaguchi T, Tsuboi T.
  • Journal Title: The Journal of Physiological Sciences 59 Pages: 301-301
• [Journal Article] Monocarboxylate transporter-2 (MCT2) regulates subcellular localization and surface expression of glutamate receptor-2 (GluR2). (2009)
  • Author(s): Maekawa F, Tsuboi T, Yada T, Pellerin L.
  • Journal Title: The Journal of Physiological Sciences 59 Pages: 136-136
• [Journal Article] Molecular mechanism of attachment process of dense-core vesicles to the plasma membrane in neuroendocrine cells. (2009)
  • Author(s): Tsuboi T.
  • Journal Title: Neuroscience Research 63 Pages: 83-88
  • NAID: 10027499443
  • Peer Reviewed
• [Journal Article] Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain. (2009)
  • Author(s): Ohata S, Kinoshita S, Aoki R, Tanaka H, Wada H, Tsuruoka-Kinoshita S, Tsuboi T, Watabe S, Okamoto H.
  • Journal Title: Development 136 Pages: 1653-1663
  • Peer Reviewed
• [Journal Article] Regulation of the intracellular distribution, cell surface expression, and protein levels of AMPA receptor GluR2 subunits by the monocarboxylate transporter MCT2 in neuronal cells. (2009)
  • Author(s): Maekawa F, Tsuboi T, Fukuda M, Pellerin L.
  • Journal Title: Journal of Neurochemistry 109 Pages: 1767-1778
  • Peer Reviewed
• [Journal Article] Regulation of the intracellular distribution, cell surface expression, and protein levels of AMPA receptor GluR2 subunits by the monocarboxylate transporter MCT2 in neuronal cells (2009)
  • Author(s): Maekawa F, Tsuboi T, Fukuda M, Pellerin L.
  • Journal Title: Journal of Neurochemistry 109 Pages: 1767-1778
  • Peer Reviewed
• [Journal Article] Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain (2009)
  • Author(s): Ohata S, Kinoshita S, Aoki R, Tanaka H, Wada H, Tsuruoka-Kinoshita S, Tsuboi T, Watabe S, Okamoto H
  • Journal Title: Development 136 Pages: 1653-1663
  • Peer Reviewed
• [Journal Article] Molecular mechanism of attachment process of dense-core vesicles to the plasma membrane in neuroendocrine cells (2009)
  • Author(s): Tsuboi T
  • Journal Title: Neuroscience Research 63 Pages: 83-88
  • NAID: 10027499443
  • Peer Reviewed
• [Presentation] 産生時間依存的な開口放出制御機構の解析 (2011)
  • Author(s): 坪井貴司、北口哲也、唐澤智司、福田光則、宮脇敦史
  • Organizer: 第88回日本生理学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] 産生時間依存的な開口放出制御機構の解析 (2011)
  • Author(s): 坪井貴司、北口哲也、唐澤智司、福田光則、宮脇敦史
  • Organizer: 第88回日本生理学会
  • Place of Presentation: 横浜(パシフィコ横浜会議センター)
  • Year and Date: 2011-03-28
• [Presentation] Model Guided Quantification of Exocytotic Vesicle Dynamics. (2010)
  • Author(s): Lee SJ, Oh S, Huang C, Watanabe H, Tsuboi T.
  • Organizer: 50th American Society for Cell Biology
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2010-12-12
• [Presentation] Model Guided Quantification of Exocytotic Vesicle Dynamics (2010)
  • Author(s): Lee SJ, Oh S, Huang C, Watanabe H, Tsuboi T
  • Organizer: 50th American Society for Cell Biology
  • Place of Presentation: Philadelphia, PA, USA (Pennsylvania Convention Center)
  • Year and Date: 2010-12-12
• [Presentation] 海馬神経細胞におけるAMPAが誘導する核内Ca2+上昇と核内穎粒出現に対するIP3 signalingの解析 (2010)
  • Author(s): 茨木京子、山本清二、坪井貴司、寺川進
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] 海馬神経細胞におけるAMPAが誘導する核内Ca2+上昇と核内顆粒出現に対するIP3 signalingの解析 (2010)
  • Author(s): 茨木京子、山本清二、坪井貴司、寺川進
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸(神戸コンベンションセンター)
  • Year and Date: 2010-09-03
• [Presentation] 産生時間依存的な開口放出制御機構の解析 (2010)
  • Author(s): 坪井貴司、北口哲也、唐澤智司、福田光則、宮脇敦史
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] ミオシン2は表層繊維状アクチンの再編成を介して融合孔の開いている時間を制御する (2010)
  • Author(s): 青木亮、北口哲也、宮脇敦史、坪井貴司
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] 蛍光プローブを用いた膵β細胞におけるグルタミン酸誘導性インスリン分泌の可視化解析 (2010)
  • Author(s): 大屋愛実、佐藤舞、鈴木秀幸、佐藤守俊、坪井貴司
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] 産生時間依存的な開口放出制御機構の解析 (2010)
  • Author(s): 坪井貴司、北口哲也、唐澤智司、福田光則、宮脇敦史
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡(盛岡市民文化ホール)
  • Year and Date: 2010-05-20
• [Presentation] 蛍光プローブを用いた膵β細胞におけるグルタミン酸誘導性インスリン分泌の可視化解析 (2010)
  • Author(s): 大屋愛実、佐藤舞、鈴木秀幸、佐藤守俊、坪井貴司
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡(盛岡市民文化ホール)
  • Year and Date: 2010-05-20
• [Presentation] オシン2は表層繊維状アクチンの再編成を介して融合孔の開いている時間を制御する (2010)
  • Author(s): 青木亮、北口哲也、宮脇敦史、坪井貴司
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡(盛岡市民文化ホール)
  • Year and Date: 2010-05-20
• [Presentation] マクロファージ由来サイトカイン分泌機構の解析 (2009)
  • Author(s): 森亮一、池松和哉、下川功、坪井貴司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Actin filament functions as an exocytosis promoting factor in neuroendocrine cells (2009)
  • Author(s): 佐藤舞、坪井貴司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Different peptide hormone release dynamics on astrocytes (2009)
  • Author(s): 柳原優、坪井貴司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Glucose-induced IP3 signal in pancreatic MIN6 cells revealed by GFP-based FRET probe (2009)
  • Author(s): 大屋愛実、鈴木秀幸、佐藤守俊、坪井貴司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Automated kinetic characterization of exocytotic events in total internal reflection microscopy (2009)
  • Author(s): Lee JSJ, Huang CC, Kenyon Z, Watanabe H, Tsuboi T.
  • Organizer: 49th American Society for Cell Biology
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2009-12-06
• [Presentation] Myosin II modulates a size of exocytotic fusion pore in neuroendocrine cells (2009)
  • Author(s): Aoki R, Kitaguchi T, Miyawaki A, Tsuboi T.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] Myosin II modulates a size of exocytotic fusion pore in neuroendocrine cells (2009)
  • Author(s): Aoki R, Kitaguchi T, Miyawaki A, Tsuboi T
  • Organizer: 第32回 日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] Monocarboxylate transporter-2 (MCT2) regulates subcellular localization and surface expression of glutamate receptor-2 (GluR2). (2009)
  • Author(s): Maekawa F, Tsuboi T, Yada T, Pellerin L.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-29
• [Presentation] Myosin II regulates "kiss-and-run" dense-core exocytosis revealed by total internal reflection fluorescence microscopy. (2009)
  • Author(s): Aoki R, Kitaguchi T, Tsuboi T.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] Myosin II regulates "kiss-and-run" dense-core exocytosis revealed by total internal reflection fluorescence microscopy (2009)
  • Author(s): Aoki R, Kitaguchi T, Tsuboi T
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] Monocarboxylate transporter-2(MCT2)regulates subcellular localization and surface expression of glutamate receptor-2 (GluR2) (2009)
  • Author(s): 前川文彦, 坪井貴司, 矢田俊彦, Luc Pellerin
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-27
• [Presentation] 乳酸輸送体MCT2がグルタミン酸AMPA受容体サブタイプGluR2の細胞膜表面発現に及ぼす影響 (2009)
  • Author(s): 前川文彦, 坪井貴司, 矢田俊彦, Luc Pellerin.
  • Organizer: 第52回日本糖尿病学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-21
• [Book] 図説生物学 (2010)
  • Author(s): 坪井貴司
  • Publisher: 東京大学出版
• [Book] ベーシックマスター細胞生物学 (2010)
  • Author(s): 坪井貴司
  • Publisher: オーム社
• [Book] 図説生物学「第4章動物の情報処理」「第5章動物の代謝」 (2010)
  • Author(s): 坪井貴司
  • Publisher: 東京大学出版
• [Book] ベーシックマスター細胞生物学「第6章 核と膜小器官」 (2009)
  • Author(s): 坪井貴司
  • Publisher: オーム社
• [Remarks]
  • URL: http://secretion.bio.c.u-tokyo.ac.jp/
• [Remarks]
  • URL: http://secretion.bio.c.u-tokyo.ac.jp/~tsuboi/index.html