Structural and functional analyses of the assembly modules of ion channels for artificial protein design

• Project/Area Number: 21790201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Osaka University
• Principal Investigator: FUJIWARA Yuichiro 大阪大学, 大学院・医学系研究科, 助教 (20532980)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 生体膜 / チャネル / トランスポーター / 能動輸送 / イオンチャネル

Research Abstract

We analyzed the high-resolution crystal structure of the assembly domain of the voltage-gated H+ channel dimer. Electrophysiological analysis revealed that the temperature-sensitivity of the channel and the functional cooperativity within the dimer are regulated by the assembly domain. We also succeeded in modulating the channel function by mutating the assembly characteristic. Our results shed light on the artificial ion channel design.

Research Products

• [Journal Article] Structual and functional dynamics of the ATP receptor channel P2X(2) (2010)
  • Author(s): Kubo Y, Fujiwara Y, Keceli B, Nakajo K
  • Journal Title: Brain Nerve誌 Volume: 62巻 Pages: 1323-1329
  • Peer Reviewed
• [Journal Article] Structual and functional dynamics of the ATP receptor channel P2X(2) (2010)
  • Author(s): Kubo Y, Fujiwara Y, Keceli B, Nakajo K
  • Journal Title: Brain Nerve. Volume: 62 Pages: 1323-1329
  • Peer Reviewed
• [Journal Article] Voltage- and [ATP]-dependent gating of the P2X2 ATP receptor channel (2009)
  • Author(s): Fujiwara Y, Keceli B, Nakajo K, Kubo Y
  • Journal Title: Journal of General Physiology誌 Volume: 133巻 Pages: 93-109
  • Peer Reviewed
• [Journal Article] Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block (2009)
  • Author(s): Chatelain FC, Gazzarrini S, Fujiwara Y, Arrigoni C, Domigan C, Ferrara G, Pantoja C, Thiel G, Moroni A, Minor DL
  • Journal Title: PLoS One誌 Volume: 4巻 Pages: 7496-7502
  • Peer Reviewed
• [Journal Article] Dynamic aspects of functional regulation of the ATP receptor channel P2X2 (2009)
  • Author(s): Kubo Y, Fujiwara Y, Keceli B, Nakajo K.
  • Journal Title: Journal of Physiology誌 Volume: 587巻 Pages: 5317-5324
  • Peer Reviewed
• [Journal Article] Voltage- and [ATP]-dependent gating of the P2X2 ATP receptor channel (2009)
  • Author(s): Fujiwara Y, Keceli B, Nakajo K, Kubo Y
  • Journal Title: Journal of General Physiology 133 Pages: 93-109
  • Peer Reviewed
• [Journal Article] Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block. (2009)
  • Author(s): Chatelain FC, Gazzarrini S, Fujiwara Y, Arrigoni C, Domigan C, Ferrara G, Pantoja C, Thiel G, Moroni A, Minor DL
  • Journal Title: PLoS One 4 Pages: 7496-7496
  • Peer Reviewed
• [Journal Article] Dynamic aspects of functional regulation of the ATP receptor channel P2X2. (2009)
  • Author(s): Kubo Y, Fujiwara Y, Keceli B, Nakajo K.
  • Journal Title: Journal of Physiology 587 Pages: 5317-24
  • Peer Reviewed
• [Presentation] 電位依存性プロトンチャネルの温度感受性ゲーティングは2量体会合構造安定性により決定する (2011)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)
  • Year and Date: 2011-03-28
• [Presentation] Stability of the Cytoplasmic Dimer Assembly Regulates the Thermosensitive Gating of the Voltage-gated H+ Channel (2011)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
  • Organizer: 第55回生物物理学会大会(米国)
  • Place of Presentation: ボルチモア(米国)(招待講演)
  • Year and Date: 2011-03-08
• [Presentation] 電位依存性プロトンチャネル細胞内領域の結晶構造と機能的意義 (2010)
  • Author(s): 藤原祐一郎、岡村康司
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 愛知(岡崎)(招待講演)
  • Year and Date: 2010-09-16
• [Presentation] 電位依存性プロトンチャネルの温度感受性ゲーティングは2量体会合構造安定性により決定する (2010)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Stability of the Cytoplasmic Dimer Assembly Regulates the Thermosensitive Gating of the Voltage-gated H+ Channel (2010)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
  • Organizer: 第55回生物物理学会大会
  • Place of Presentation: 米国 ボルチモア(招待講演)
  • Year and Date: 2010-03-08
• [Presentation] 電位依存性プロトンチャネル細胞内領域の結晶構造と機能的意義 (2009)
  • Author(s): 藤原祐一郎、岡村康司
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 岡崎(招待講演)
  • Year and Date: 2009-09-16
• [Presentation] Voltage- and [ATP]-dependent gating of the P2X2 ATP receptor channel (2009)
  • Author(s): 久保義弘、藤原祐一郎、Batu Keceli, 中條浩一
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都
  • Year and Date: 2009-07-31
• [Presentation] oltage- and [ATP]-dependent gating of the P2X2 ATP receptor channel (2009)
  • Author(s): 久保義弘、藤原祐一郎、Batu Keceli, 中條浩一
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都
  • Year and Date: 2009-07-30
• [Book] ATP受容体チャネルP2X2の膜上発現密度依存性と膜電位依存性、生体の科学「伝達物質と受容体」 (2009)
  • Author(s): 藤原祐一郎、Batu Keceli、中條浩一、山本友美、久保義弘
  • Publisher: 医学書院
• [Book] 生体の科学「伝達物質と受容体」分担執筆「ATP受容体チャネルP2X2の膜上発現密度依存性と膜電位依存性」 (2009)
  • Author(s): 藤原祐一郎、Batu Keceli, 中條浩一、山本友美、久保義弘
  • Total Pages: 4
  • Publisher: 医学書院
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/tougouseiri/menu.htm