Regulation of L-type Ca^<2+> channel activity by calmodulin

• Project/Area Number: 21790206
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Kagoshima University
• Principal Investigator: MINOBE Etsuko Kagoshima University, 医歯学総合研究科, 助教 (00448581)
• Research Collaborator: MORI Masayuki 福岡大学, 医学部, 講師 (80342640)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: カルシウムチャネル / カルモジュリン / パッチクランプ法 / inside-out / run-down / カルシウムイオン / ATP / 心筋細胞 / Caチャネル

Research Abstract

We measured activity of L-type Ca^<2+> channel and its mutants, C-terminal deletion (delta CT) and delta CT linked with calmodulin (CaM), using patch-clamp technique (inside-out mode). We found that binding of CaM to the channel was required for the channel activity. Unlike the wild type channel, the mutants did not require ATP for the channel activity. It was suggested that CaM and ATP interacted with a proximal part of C-terminal thereby producing the channel activity, which was modulated negatively by a distal part of C-terminal.

Research Products

• [Journal Article] Interactions of Calmodulin with the multiple binding sites of Cav1.2 Ca^<2+> channels. (2010)
  • Author(s): Hadhimulya Asmara, Etsuko Minobe, Saud A.Zahangir, Masaki Kameyama.
  • Journal Title: Journal of pharmacological sciences Vol.112 Pages: 397-404
  • NAID: 10027908931
  • Peer Reviewed
• [Journal Article] Calmodulin- and Ca^<2+>-dependent faclilitation and inactivation of the Cav1.2 Ca^<2+> channels in guinea-pig ventricular myocytes. (2010)
  • Author(s): Don-yun Han, Etsuko Minobe, Wu-yang Wang, Feng Guo, Jian-jun Xu, Li-ying Hao, Masaki Kameyama.
  • Journal Title: Journal of pharmacological sciences. Vol.112 Pages: 310-319
  • Peer Reviewed
• [Journal Article] Both N- and C-lobes of calmodulin are required for Ca^<2+>-dependent regulations of Cav1.2 Ca^<2+> channels. (2010)
  • Author(s): Feng Guo, Etsuko Minobe, Kazuto Yazawa, Hadhimulya Asmara, Xiao-yan Bai, Dong-yun Han, Li-ying Hao, Masaki Kameyama
  • Journal Title: Biochemical and biophysical research communications. Vol.391 Pages: 1170-1176
  • Peer Reviewed
• [Journal Article] Calmodulin- and Ca^<2+>-dependent facilitation and inactivation of the Cav1.2 Ca^<2+> channels in guinea-pig ventricular myocytes. (2010)
  • Author(s): Don-Yun Han
  • Journal Title: Journal of pharmacological sciences Volume: 112 Pages: 310-319
  • Peer Reviewed
• [Journal Article] Interactions of Calmodulin with the multiple binding sites of Cav1.2 Ca^<2+> channels (2010)
  • Author(s): Hadhimulya Asmara
  • Journal Title: Journal of pharmacological sciences Volume: 112 Pages: 397-404
  • Peer Reviewed
• [Journal Article] Both N- and C-lobes of calmodulin are required for Ca^<2+> dependent regulations of CaV1.2 Ca^<2+> channels (2010)
  • Author(s): Feng Guo
  • Journal Title: Biochemical and biophysical research communications 391 Pages: 1170-1176
  • Peer Reviewed
• [Journal Article] The distinct roles of calmodulin and calmodulin kinase II in the reversal of run-down of L-type Ca^<2+> channels in guinea-pig ventricular myocytes (2010)
  • Author(s): Li-ying Hao
  • Journal Title: Journal of pharmacological sciences 111 Pages: 416-425
  • Peer Reviewed
• [Journal Article] CaMKII phosphorylates a threonine residue in the C-terminal tail of Cav1.2 Ca^<2+> channel and modulates the interaction of the channel with calmodulin. (2009)
  • Author(s): Wu-yang Wang, Li-ying Hao, Etsuko Minobe, Zahangir A.Saud, Dong-yun Han, Masaki Kameyama
  • Journal Title: The Journal of physiological sciences. Vol.59 Pages: 283-290
  • NAID: 10025219126
  • Peer Reviewed
• [Journal Article] The distinct roles of calmodulin and calmodulin kinase II in the reversal of run-down of L-type Ca^<2+> channels in guinea-pig ventricular myocytes. (2009)
  • Author(s): Li-ying Hao, Wu-yang Wang, Etsuko Minobe, Dun-yun Han, Jian-jun Xu, Asako Kameyama, Masaki Kameyama.
  • Journal Title: Journal of pharmacological sciences. Vol.111 Pages: 416-425
  • NAID: 10026154787
  • Peer Reviewed
• [Journal Article] CaMKII phosphorylates a threonine residue in the C-terminal tail of Cav1.2 Ca^<2+> channel and modulates the interaction of the channel with calmodulin (2009)
  • Author(s): Wu-yang Wang
  • Journal Title: The journal of physiological sciences 59 Pages: 283-290
  • Peer Reviewed
• [Presentation] Ca^<2+> dependent modulation in calmodulin-linked Cav1.2 channels. (2010)
  • Author(s): Etsuko Minobe, Masayuki Mori, Jian-jun Xu, Masaki Kameyama.
  • Organizer: The Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles.
  • Place of Presentation: 鹿児島
• [Presentation] PKA-mediated phosphorylation modulates the interaction of calmodulin with cardiac Cav1.2 channels. (2010)
  • Author(s): Jian-jun Xu, Saud A.Zahangir, Yan Liu, Lei Yang, Etsuko Minobe, Asako Kameyama, Kazuto Yazawa, Masaki Kameyama.
  • Organizer: The Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles.
  • Place of Presentation: 鹿児島
• [Presentation] カルモジュリンをリンクさせたCav1.2型Ca^<2+>チャネルのCa^<2+>依存性調節 (2010)
  • Author(s): 蓑部悦子、森誠之、徐建軍、亀山正樹
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 岡崎
• [Presentation] Roles of calmodulin and the C-terminal tail ofCav1.2 channel in its activity. (2010)
  • Author(s): 蓑部悦子、森誠之、亀山正樹
  • Organizer: 日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] Roles of calmodulin and the C-termfinal tail of Cav1.2 channel in its activity (2010)
  • Author(s): 蓑部悦子
  • Organizer: 日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] Ca^<2+> dependent modulation in calmodulin-linked Cav1.2 channels. (2010)
  • Author(s): Etsuko Minobe
  • Organizer: The Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: 鹿児島
• [Presentation] PKA-mediated phosphorylation modulates the interaction of calmodulin with cardiac Cav1.2 channels. (2010)
  • Author(s): Jian-Jun Xu
  • Organizer: The Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: 鹿児島
• [Presentation] カルモジュリンの心筋Ca^<2+>チャネル(Cav1.2)に対する二重作用 (2009)
  • Author(s): 亀山正樹、Hadhimulya Asmara、蓑部悦子、Zahangir A.Saud、韓冬雲、郭鳳、王午陽、はお麗英
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 岡崎
• [Presentation] Multipule molecules of calmodulin bind to the Cav1.2 Ca^<2+> channel. (2009)
  • Author(s): Hadhimulya Asmara, Etsuko Minobe, Zahangir A.Saud, Masaki Kameyama.
  • Organizer: 西日本生理学会
  • Place of Presentation: 福岡
• [Presentation] Regulation of Cav1.2 Ca^<2+> channels by Ca^<2+>, calmodulin and CaMKII. (2009)
  • Author(s): Masaki Kameyama, Li-ying Hao, Etsuko Minobe, Jian-jun Xu, Dong-yun Han, Hong-guang Nie, Zahangir A.Saud, Wu-yang Wang, Hadhimulya Asmara, Kazuto Yazawa.
  • Organizer: The IUPS 2009 satellite symposium.
  • Place of Presentation: 京都
• [Presentation] Calpastatin domain L competes with calmodulin on IQ region of Cav1.2 channel (2009)
  • Author(s): Etsuko Minobe, Zahangir A.Saud, Dong-yun Han, Hadhimulya Asmara, Wu-yang Wang, Li-ying Hao, Masaki Kameyama.
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: 京都
• [Presentation] Facilitatory and inhibitory effects of calmodulin on activity of Cav1.2 Ca^<2+> channel. (2009)
  • Author(s): Dong-yun Han, Li-ying Hao, Etsuko Minobe, Wu-yang Wang, Jian-jun Xu, Masaki Kameyama.
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: 京都
• [Presentation] PKA modulates the effects of calmodulin on L-type Ca^<2+> channels in guinea-pig ventricular myocytes. (2009)
  • Author(s): Zahangir A.Saud, Yan Liu, Etsuko Minobe, Kazuto Yazawa, Masaki Kameyama.
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: 京都
• [Presentation] Interaction of calmodulin with the Cav1.2 Ca^<2+> channel. (2009)
  • Author(s): Hadhimulya Asmara, Etsuko Minobe, Zahangir A.Saud, Wu-yang Wang, Masaki Kameyama
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: 京都
• [Presentation] Ca^<2+> channels and cardiac functions. (2009)
  • Author(s): Masaki Kameyama, Li-ying Hao, Etsuko Minobe, Jian-jun Xu, Dong-yun Han, Hong-guang Nie, Zahangir A.Saud, Wu-yang Wang, Hadhimulya Asmara.
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: 京都
• [Presentation] Calpastatin domain L competes with calmodulin on IQ region of Cav1.2 channel (2009)
  • Author(s): Etsuko Minobe
  • Organizer: International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] Facilitatory and inhibitory effects of calmodulin on activity of Cav1.2 Ca^<2+> channel (2009)
  • Author(s): Dong-yun Han
  • Organizer: International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] PKA modulates the effects of calmodulin on L-type Ca^<2+> channels in guinea-pig ventricular myocytes (2009)
  • Author(s): Zahangir A.Saud
  • Organizer: International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] Interaction of calmodulin with the Cav1.2 Ca^<2+> channel (2009)
  • Author(s): Hadhimulya Asmara
  • Organizer: International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] Ca^<2+> channels and cardiac functions (2009)
  • Author(s): Masaki Kameyama
  • Organizer: International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] Regulation of Cav1.2 Ca^<2+> channels by Ca^<2+>, calmodulin and CaMKII (2009)
  • Author(s): Masaki Kameyama
  • Organizer: The IUPS 2009 satellite symposium
  • Place of Presentation: 京都
• [Presentation] Multipule molecules of calmodulin bind to the Cav1.2 Ca^<2+> channel (2009)
  • Author(s): Hadhimulya Asmara
  • Organizer: 西日本生理学会
  • Place of Presentation: 福岡
• [Presentation] カルモジュリンの心筋Ca^<2+>チャネル(Cav1.2)に対する二重作用 (2009)
  • Author(s): 亀山正樹
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 岡崎市(愛知県)
• [Remarks] ホームページ等
  • URL: http://www.kufm.kagoshima-u.ac.jp/~physiol2/
• [Remarks]
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  • URL: http://www.kufm.kagoshima-u.ac.jp/~physiol2/