| Project/Area Number |
21790206
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
MINOBE Etsuko Kagoshima University, 医歯学総合研究科, 助教 (00448581)
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| Research Collaborator |
MORI Masayuki 福岡大学, 医学部, 講師 (80342640)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | カルシウムチャネル / カルモジュリン / パッチクランプ法 / inside-out / run-down / カルシウムイオン / ATP / 心筋細胞 / Caチャネル |
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| Research Abstract |
We measured activity of L-type Ca^<2+> channel and its mutants, C-terminal deletion (delta CT) and delta CT linked with calmodulin (CaM), using patch-clamp technique (inside-out mode). We found that binding of CaM to the channel was required for the channel activity. Unlike the wild type channel, the mutants did not require ATP for the channel activity. It was suggested that CaM and ATP interacted with a proximal part of C-terminal thereby producing the channel activity, which was modulated negatively by a distal part of C-terminal.
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