Effects of extracelluIar chloride on regulatory mechanisms of the epithelial sodium channels

• Project/Area Number: 21790210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Fukuoka University
• Principal Investigator: YAMADA Toshiki Fukuoka University, 学長付, 助教 (10453101)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ナトリウム輸送 / ENaC / クロライドイオン / パッチクランプ / Na^+輸送 / 電気生理学

Research Abstract

To determine regulatory mechanisms of epithelial sodium channels by extracellular chloride, electrophysiological experiments were performed with distal nephrone epithelial cells from Xenopus larvls, A6 cells. Lowing extracellular Cl^- concentration decreased mean open time and open probability of ENaC. These results suggest that extracellular Cl^- plays a key role in control of the ENaC-mediated Na^+ reabsorption.

Research Products

• [Journal Article] NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulate paracellular transport. (2010)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun 393(3) Pages: 390-396
  • Peer Reviewed
• [Journal Article] The role of sodium, calcium, and magnesium transport in the renal distal tubule. (2009)
  • Author(s): Yamada T, Kita S, Iyoda T, Yamamoto S, Iwamoto T.
  • Journal Title: Med.Bull.Fukuoka Univ 36(4) Pages: 257-262
  • NAID: 110007376606
  • Peer Reviewed
• [Journal Article] Role of C1 channels and transporters in cardiac cell volume homeostasis. (2009)
  • Author(s): Yamamoto S, Yamada T, Iyoda T, Kita S, Iwamoto T.
  • Journal Title: Med.Bull.Fukuoka Univ 36(4) Pages: 243-255
  • Peer Reviewed
• [Journal Article] Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellularion transport. (2009)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun. 390(4) Pages: 1315-1321
• [Journal Article] Regulation of paracellular NH^+ and C1 conductances by hydrostatic pressure. (2009)
  • Author(s): Tokuda S, Niisato N, Nagai T, Taruno A, Nakajima K, Miyazaki H, Yamada T, Hosogi S, Ohta M, Nishio K, Iwasaki Y, Marunaka Y.
  • Journal Title: Cell Biol Int. 33(9) Pages: 949-956
  • Peer Reviewed
• [Journal Article] Effect of chloride ion on epithelial sodium channels (ENaC) in AVT-stimulated renal epithelial cells. (2009)
  • Author(s): Yamada T, Niisato N, Marunaka Y.
  • Journal Title: J Physiol Sci 59 Suppl 1 Pages: 295-295
  • Peer Reviewed
• [Journal Article] Effects of extracellular chloride ion on epithelial sodium channel (ENaC) in arginine vasotocin (AVT)-stimulated renal epithelial cells. (2009)
  • Author(s): Yamada T, Niisato N, Marunaka Y.
  • Journal Title: Biomed Res 30(3) Pages: 193-198
  • NAID: 130004470752
  • Peer Reviewed
• [Journal Article] Effects of extracellular chloride ion on epithelial sodium channel(ENaC)in arginine vasotocin(AVT)-stimulated renal epithelial cells (2009)
  • Author(s): Toshiki Yamada
  • Journal Title: Biomedical Research 30(3) Pages: 193-198
  • NAID: 130004470752
  • Peer Reviewed
• [Presentation] 2種のカエル腹皮のイオン輸送におけるバソトシンの作用 (2009)
  • Author(s): 山田敏樹、内山実、岩本隆宏
  • Organizer: トランスポーター研究会第3回几州部会
  • Place of Presentation: 鹿児島
  • Year and Date: 2009-11-21
• [Presentation] 腎上皮細胞の抗利尿ホルモン促進性Na^+輸送における細胞外C1の影響 (2009)
  • Author(s): 山田敏樹、新里直美、丸中良典、岩本隆宏
  • Organizer: TRANSPOTSOME平成21年度第1回班会議
  • Place of Presentation: 熊本
  • Year and Date: 2009-08-26
• [Presentation] Effect of chloride ion on epithelial sodium channels (ENaC) in AVT-stimulated renal epithelial cells. (2009)
  • Author(s): Yamada T, Niisato N, Marunaka Y.
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-29
• [Presentation] Effects of chloride ion on epithelial sodium channels(ENaC)in arginine vasotocin(AVT)-stimulated renal epithelial cells (2009)
  • Author(s): Toshiki Yamada
  • Organizer: The XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-29
• [Book] In : Frogs : Biology, Ecology and Uses.(Hormonal regulation of ion transports in frog epithelia-Measurements of ion transport in frog epithelium using electrophysiological techniques)(Editor James L.Murray.)ISBN:978-1-61324-667-2 (2011)
  • Author(s): Yamada T, Uchiyama M.
  • Publisher: Nova science Publishers