Identification of the anion channel involved in volume regulation in the principal cells of rat cortical collecting duct.

• Project/Area Number: 21790212
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Iwate Medical University
• Principal Investigator: KOMAGIRI You Iwate Medical University, 医学部, 助教 (80405753)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 生体膜 / チャネル / トランスポーター / 能動輸送 / 生理学 / 細胞・組織 / クロライドチャネル / 低浸透圧

Research Abstract

Anion efflux and intracellular Ca^<2+> increase have been known to play important roles in cell volume regulation during hypotonicity. This study was carried out to explore the mechanisms of hypotonicity-induced intracellular Ca^<2+> elevation and characterize anion conductance activated by hypotonicity in the principal cells of freshly isolated rat cortical collecting ducts. Using Fura-2 fluorescence Ca^<2+> imaging and whole-cell voltage-clamp techniques, it was found that a nicardipine-sensitive Ca^<2+> entry pathway was involved in the mechanisms underlying the hypotonicity-induced intracellular Ca^<2+> increase and a NPPB-sensitive/glibenclamide-insensitive Cl^- current was activated by hypotonic stimulation in the principal cells of rat cortical collecting ducts.

Research Products

• [Journal Article] A nicardipine-sensitive Ca^<2+> entry contributes to the hypotonicity-induced increase in [Ca^<2+>]_i of principal cells in rat cortical collecting duct. (2011)
  • Author(s): Komagiri Y, Nakamura K, Kubokawa M.
  • Journal Title: Cell Calcium. 49 Pages: 35-42
  • Peer Reviewed
• [Journal Article] A nicardipine-sensitive Ca^<2+> entry contributes to the hypotonicity-induced increase in [Ca^<2+>]_i of principal cells in rat cortical collecting duct. (2011)
  • Author(s): Komagiri Y, Nakamura K, Kubokawa M.
  • Journal Title: Cell Calcium Volume: 49 Pages: 35-42
  • Peer Reviewed
• [Presentation] Characterization of hypotonicity-induced channel activation and [Ca^<2+>]_i elevation of principal cells in rat kidney cortical collecting ducts. (2011)
  • Author(s): Komagiri Y, Nakamura K, Kubokawa M.
  • Organizer: 第88回日本生理学会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] Characterization of hypotonicity-induced channel activation and [Ca^<2+>]_i elevation of principal cells in rat kidney cortical collecting ducts. (2011)
  • Author(s): Komagiri Y, Nakamura K, Kubokawa M.
  • Organizer: 第88回日本生理学会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] Involvement of extracellular Ca^<2+> entry through a nicardipine-sensitive pathway in the hypotonicity- induced increase in [Ca^<2+>]_i of principal cells in rat cortical collecting duct. (2010)
  • Author(s): Komagiri Y, Nakamura K, Kubokawa M.
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] Involvement of extracellular Ca^<2+> entry through a nicardipine-sensitive pathway in the hypotonicity-induced increase in [Ca^<2+>]_i of principal cells in rat cortical collecting duct. (2010)
  • Author(s): Komagiri Y, Nakamura K, Kubokawa M.
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] Hypotonicity-induced [Ca^<2+>]_i elevation of principal cells in isolated rat kidney cortical collecting ducts. (2009)
  • Author(s): Komagiri Y, Kojo T, Nakamura K, Kubokawa M.
  • Organizer: 国際合同シンポジウム:アニオン輸送と細胞容積調節(PAT-CVR 2009)
  • Place of Presentation: 岡崎
  • Year and Date: 2009-08-04
• [Presentation] A Ca^<2+> entry pathway for the hypotonicity-induced elevation in [Ca^<2+>]_i of principal cells in isolated rat kidney CCDs. (2009)
  • Author(s): Komagiri Y, Kojo T, Nakamura K, Kubokawa M.
  • Organizer: 第36回国際生理学会世界大会(IUPS 2009)
  • Place of Presentation: 京都
  • Year and Date: 2009-07-31
• [Presentation] 学ラット腎皮質集合管における低浸透圧刺激時の主細胞内Ca^<2+>濃度上昇における細胞外Ca^<2+>流入経路の検討 (2009)
  • Author(s): 駒切洋、古城俊之、中村一芳、久保川
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-03
• [Remarks] ホームページ等
• [Remarks] 岩手医科大学医学部生理学講座器官生理学分野HP
  • URL: http://physiology2.iwate-med.ac.jp/indexj.htm