The identification of a novel mechanism of osteoclast differentiation that is calcium oscillation-independent osteoclastogenesis

• Project/Area Number: 21790220
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KURODA Yukiko The Institute of Physical and Chemical Research, 発生神経生物研究チーム, 基礎科学特別研究員 (70455343)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分化 / 破骨細胞 / カルシウムオシリーション / 細胞融合 / シグナル伝達 / イノシトール三リン酸(IP3) / カルシウムオシレーション / 骨 / イノシトール3リン酸 (IP3) / IRBIT

Research Abstract

By analysis of osteoclasts derived from IRBIT knockout mice, we found that the number of RANKL-induced Ca^<2+> oscillation positive cells was increased in IRBIT knockout mononuclear osteoclasts and that IRBIT knockout cells enhanced cell-cell fusion step during osteoclastogenesis as compared with wild-type cells. These results indicate that IRBIT negatively regulates IP_3R-mediated Ca^<2+> signaling and cell-cell fusion step during osteoclast differentiation.

Research Products

• [Journal Article] Inositol 1, 4, 5-triphosphate receptor-binding protein released with inositol 1, 4, 5-triphosphate (IRBIT) associates with components of the mRNA 3' processing machinery in a phosphorylation -dependent manner and inhibits polyadenylation. (2009)
  • Author(s): Kiefer H, Mizutani A, Iemura S, Natsume T, Ando H, Kuroda Y, Mikoshiba K.
  • Journal Title: J Biol Chem. 284(16) Pages: 10694-10705
  • Peer Reviewed
• [Journal Article] Inositol 1, 4, 5-triphosphate receptor-binding protein released with Inositol 1, 4, 5-triphosphate(IRBIT), associates with components of the mRNA 3'processing machinery in a phosphorylation-dependent manner and inhibits polyadenylation (2009)
  • Author(s): Kiefer H
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 10694-10705
  • Peer Reviewed
• [Presentation] 御子柴克彦生体内における破骨細胞分化はカルシウムオシレーション依存的・および非依存的メカニズムによって制御されている (2009)
  • Author(s): 黒田有希子、久恒智博、中村健、松尾光一
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Presentation] 生体内における破骨細胞分化なカルシウムオシレーション依存的・および非依存的メカニズムによって制御されている (2009)
  • Author(s): 黒田有希子
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Presentation] IRBITの中枢神経系における分布について (2009)
  • Author(s): 水谷顕洋、御子柴克彦、安東英明、黒田査孟王、河合克宏
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16