The elucidation of molecular basis on mechanisms for chemosensitive respiratory regulation

• Project/Area Number: 21790231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hyogo College of Medicine
• Principal Investigator: HIRATA Yutaka Hyogo College of Medicine, 医学部, 助教 (10441247)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: TRP / 中枢化学受容器 / グリア細胞 / カルシウム応答 / 二酸化炭素 / 水素イオン濃度 / 延髄 / 培養細胞

Research Abstract

The medulla contains central chemosensitive cells (CRC) important for the maintenance of blood gas and pH homeostasis. To identify the intrinsic CRC, we measured responses of intracellular Ca^<2+> and H^+, and membrane potential of rat primary-cultured medullary cells to a brief exposure (sec level) to hypercapnic acidosis. In glia-rich cultures, intracellular acidification preceded the hypercapnic acidosis-induced Ca^<2+> response independent of extracellular pH. TRP channel blockers attenuated the hypercapnic acidosis-induced Ca^<2+> response. Subpopulations of cells that exhibited the hypercapnic acidosis-induced Ca^<2+> response also responded to the application of TRP agonist. These results suggest that the TRP channel is a potent candidate of central chemosensing molecules.

Research Products

• [Journal Article] TRP channels are involved in mediating hypercapnic Ca^<2+> responses in rat glia-rich medullary cultures independently of extracellular pH. (2010)
  • Author(s): Hirata Y, Oku Y.
  • Journal Title: Cell Calcium 48 Pages: 124-142
  • Peer Reviewed
• [Journal Article] TRP channels are involved in mediating hypercapnic Ca2+ responses in rat glia-rich medullary cultures independent of extracellular pH. (2010)
  • Author(s): Hirata Y, Oku Y.
  • Journal Title: Cell Calcium Volume: 48 Pages: 124-132
  • Peer Reviewed
• [Journal Article] Ca2+ overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle. (2010)
  • Author(s): Zhao X, Yamazaki D, Park KH, Komazaki S, Tjondrokoesoemo A, Nishi M, Lin P, Hirata Y, Brotto M, Takeshima H, Ma J.
  • Journal Title: Journal of Biological Chemistry Volume: 26 Pages: 37370-37376
  • Peer Reviewed