| Project/Area Number |
21790231
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
HIRATA Yutaka Hyogo College of Medicine, 医学部, 助教 (10441247)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | TRP / 中枢化学受容器 / グリア細胞 / カルシウム応答 / 二酸化炭素 / 水素イオン濃度 / 延髄 / 培養細胞 |
|---|
| Research Abstract |
The medulla contains central chemosensitive cells (CRC) important for the maintenance of blood gas and pH homeostasis. To identify the intrinsic CRC, we measured responses of intracellular Ca^<2+> and H^+, and membrane potential of rat primary-cultured medullary cells to a brief exposure (sec level) to hypercapnic acidosis. In glia-rich cultures, intracellular acidification preceded the hypercapnic acidosis-induced Ca^<2+> response independent of extracellular pH. TRP channel blockers attenuated the hypercapnic acidosis-induced Ca^<2+> response. Subpopulations of cells that exhibited the hypercapnic acidosis-induced Ca^<2+> response also responded to the application of TRP agonist. These results suggest that the TRP channel is a potent candidate of central chemosensing molecules.
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