Visualization of stress reaction in neuroendocrine system using multiple fluorescent protein transgenic rats.
| Project/Area Number |
21790232
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | The University of Tokushima (2010)
University of Occupational and Environmental Health, Japan (2009) |
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Principal Investigator |
FUJIHARA Hiroaki The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (10369051)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | トランスジェニック / ラット / RFP / ストレス |
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| Research Abstract |
I examined the effect of osmotic stimulus using c-fos-mRFP transgenic rats, c-fos-mRFP/arginine vasopressin-eGFP double transgenic rats and oxytocin-mRFP transgenic rats. In all transgenic rats, fluorescence and mRNA levels of the c-fos-mRFP, arginine vasopressin-eGFP and oxytocin-mRFP were increased after osmotic stimulus. I succeeded in visualization of stress reaction in neuroendocrine system as index of green fluorescence and red fluorescence.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Specific expression of an oxytocin- enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary.2010
Author(s)
Katoh A, Fujihara H, Ohbuchi T, Onaka T, Young WS 3rd, Dayanithi G, Yamasaki, Kawata M, Suzuki H, Otsubo H, Suzuki H, Murphy D, Ueta Y.
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Journal Title
Journal of Endocrinology 204(3)
Pages: 275-285
Related Report
Peer Reviewed
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[Journal Article] Induction of the arginine vasopressin- enhanced green fluorescent protein fusion transgene in the rat locus coeruleus.2010
Author(s)
Todoroki M, Ueta Y, Fujihara H, Otsubo H, Shibata M, Hashimoto H, Kobayashi M, Sakamoto H, Kawata M, Dayanithi G, Murphy D, Hiro H, Takahashi K, Nagata S.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Exaggerated response of a vasopressin- enhanced green fluorescent protein transgene to nociceptive stimulation in the rat.2009
Author(s)
Suzuki H, Kawasaki M, Ohnishi H, Otsubo H, Ohbuchi T, Katoh A, Hashimoto H, Yokoyama T, Fujihara H, Dayanithi G, Murphy D, Nakamura T, Ueta Y.
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Journal Title
The Journal of Neuroscience 29(42)
Pages: 13182-13189
Related Report
Peer Reviewed
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