| Project/Area Number |
21790232
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | The University of Tokushima (2010)
University of Occupational and Environmental Health, Japan (2009) |
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Principal Investigator |
FUJIHARA Hiroaki The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (10369051)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | トランスジェニック / ラット / RFP / ストレス |
|---|
| Research Abstract |
I examined the effect of osmotic stimulus using c-fos-mRFP transgenic rats, c-fos-mRFP/arginine vasopressin-eGFP double transgenic rats and oxytocin-mRFP transgenic rats. In all transgenic rats, fluorescence and mRNA levels of the c-fos-mRFP, arginine vasopressin-eGFP and oxytocin-mRFP were increased after osmotic stimulus. I succeeded in visualization of stress reaction in neuroendocrine system as index of green fluorescence and red fluorescence.
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