The role of the excitatory action of GABA in the mouse estrous cycle.

• Project/Area Number: 21790234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Gunma University (2010); National Institute for Physiological Sciences (2009)
• Principal Investigator: WATANABE Miho 群馬大学, 先端科学研究指導者育成ユニット, 助教 (10399321)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: GnRH / GABA / KCC2 / 性周期

Research Abstract

I investigated the functional consequences of excitatory action of GABA responses on GnRH neuron physiology. A transgenic mouse was generated using tetracycline controlled gene expression system. In this mouse, KCC2 was overexpressed restricted in GnRH neurons reversibly in specific time point. This transgenic mouse could not show the pregnancy. These results suggested that the excitatory action of GABA in GnRH neurons has the important role in the regulation of the estrous cycle.

Research Products

• [Journal Article] Depolarizing shift in the GABA-induced current reversal potential by lidocaine hydrochloride. (2010)
  • Author(s): Nakahata Y, Miyamoto A, Watanabe M, Moorhouse A J, Nabekura J
  • Journal Title: Brain Research Volume: 1345 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Clustering of neuronal K^+ -Cl^- cotransporters in lipid rafts by tyrosine phosphorylation. (2009)
  • Author(s): Watanabe M, Wake H, Moorhouse A, Nabekura J
  • Journal Title: Journal of Biological Chemistry Volume: 284 Pages: 27980-27988
  • Peer Reviewed
• [Journal Article] GABA A receptors mediate excitation in adult rat GnRH neurons. (2009)
  • Author(s): Watanabe M*, Sakuma Y, Kato M
  • Journal Title: Biology of Reproduction Volume: 81 Pages: 327-332
  • Peer Reviewed
• [Journal Article] Clustering of neuronal K^+-Cl^- cotransporters in lipid rafts by tyro sine phosphorylation (2009)
  • Author(s): Watanabe M, Wake H, Moorhouse A, Nabekura J
  • Journal Title: Journal of Biological Chemistry Volume: 284 Pages: 27980-27988
  • Peer Reviewed
• [Journal Article] GABA_A receptors mediate excitation in adult rat GnRH neurons (2009)
  • Author(s): Watanabe M, Sakuma Y, Kato M
  • Journal Title: Biology of Reproduction Volume: 81 Pages: 327-332
  • Peer Reviewed
• [Presentation] 発達/傷害によるカリウム-クロライド共役担体(KCC2)の機能制御 (2011)
  • Author(s): 渡部美穂、鍋倉淳一
  • Organizer: 第1回放射線神経生物学研究集会
  • Place of Presentation: 群馬大学（群馬県）
  • Year and Date: 2011-01-29
• [Presentation] 発達/傷害によるカリウム-クロライド共役担体(KCC2)の機能制御 (2011)
  • Author(s): 渡部美穂、鍋倉淳一
  • Organizer: 第1回放射線神経生物学研究集会
  • Place of Presentation: 群馬大学(群馬県)
  • Year and Date: 2011-01-29
• [Presentation] 生体脳においてGABA_A受容体の活性化は大脳皮質辺縁帯GABA作動性神経 細胞の多方向性移動を促進する (2010)
  • Author(s): 稲田浩之、渡部美穂、内田琢、福田敦夫、柳川右千夫、鍋倉淳一
  • Organizer: 第57回中部日本生理学会大会
  • Place of Presentation: 藤田保健衛生大学（愛知県）
  • Year and Date: 2010-10-16
• [Presentation] 生体脳においてGABAA受容体の活性化は大脳皮質辺縁帯GABA作動性神経細胞の多方向性移動を促進する (2010)
  • Author(s): 稲田浩之、渡部美穂、内田琢、福田敦夫、柳川右千夫、鍋倉淳一
  • Organizer: 第57回中部日本生理学会大会
  • Place of Presentation: 藤田保健衛生大学(愛知県)
  • Year and Date: 2010-10-16
• [Presentation] リドカインによるK^+-Cl^-共輸送体(KCC2)の機能抑制 (2010)
  • Author(s): 中畑義久、宮本愛喜子、渡部美穂、鍋倉淳一、石橋仁
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県）
  • Year and Date: 2010-09-03
• [Presentation] リドカインによるK^+-Cl-共輸送体(KCC2)の機能抑制 (2010)
  • Author(s): 中畑義久、宮本愛喜子、渡部美穂、鍋倉淳一、石橋仁
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-03
• [Presentation] チロシンリン酸化による神経特異的カリウムークロール共役担体の機能制御 (2010)
  • Author(s): 鍋倉淳一、渡部美穂
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 2010-05-21
• [Presentation] チロシンリン酸化による神経特異的カリウム-クロール共役担体の機能制御 (2010)
  • Author(s): 鍋倉淳一、渡部美穂
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 2010-05-21
• [Presentation] Modulation of KCC2 function by tyrosine phosphorylation. (2010)
  • Author(s): Moorhouse A, Watanabe M, Wake H, Nabekura J
  • Organizer: The 30th Annual Meeting of the Australian Neuroscience Society/ the 50th Anniversary Meeting of the Australian Physiological Society
  • Place of Presentation: Sydney convention & Exhibition Centre (Australia)
  • Year and Date: 2010-02-01
• [Presentation] Modulation of KCC2 function by tyrosine phosphorylation (2010)
  • Author(s): Moorhouse A, Watanabe M, Wake H, Nabekura J
  • Organizer: The 30th Annual Meeting of the Australian Neuroscience Society/ the 50th Anniversary Meeting of the Australian Physiological Society
  • Place of Presentation: Sydney convention & Exhibition Centre (Australia)
  • Year and Date: 2010-02-01
• [Presentation] Clustering of neuronal K^+-Cl^- cotransporter in the lipid rafts by tyrosine phosphorylation. (2009)
  • Author(s): Watanabe M, Wake H, Nabekura J
  • Organizer: 40th NIPS International Symposium-International Joint Symposium : PAT-CVR
  • Place of Presentation: 岡崎コンファレンスセンター (愛知県)
  • Year and Date: 2009-08-05
• [Presentation] Clustering of neuronal K^+-Cl^- cotransporter in the lipid rafts by tyrosine phosphorylation (2009)
  • Author(s): Watanabe M, Wake H, Nabekura J
  • Organizer: 40th NIPS International Symposium-International Joint Symposium : PAT-CVR
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2009-08-05
• [Presentation] Functional regulation of neuronal K-Cl transporter by tyrosine phosphorylation. (2009)
  • Author(s): Nabekura J and Watanabe M
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-08-01
• [Presentation] Functional regulation of neuronal K-Cl transporter by tyrosine phosphorylation (2009)
  • Author(s): Watanabe M, Wake H, Nabekura J
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-08-01
• [Remarks]
  • URL: http://asrldu.dept.med.gunma-u.ac.jp/mwatanabe/miho_watanabe.html