Involvement of oncogenes in the self-renewal of ES cells

• Project/Area Number: 21790268
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kanazawa University
• Principal Investigator: AKAGI Tadayuki Kanazawa University, 医学系, 助教 (70532183)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 胚性幹細胞 / がん遺伝子 / がん幹細胞 / ES細胞 / 自己複製 / ETV4 / ETV5 / Oct3 / 4 / MAX / EWSR1

Research Abstract

Pluripotent mouse embryonic stem (ES) cells proliferate with an undifferentiated status (i.e. self-renewal) in the presence of leukemia inhibitory factor (LIF). Cancer cells are characterized as immature malignant cells with features of aggressive proliferation and metastasis to other tissues. Interestingly, stem cells and cancer cells share similar feature : Proliferation with an undifferentiated status. This finding indicates that oncogenes might be involved in self-renewal of ES cells. In the present study, I focused on oncogenes and investigated its roles and functions in ES cells.

Research Products

• [Journal Article] In vivo Deficiency of both C/EBPβ and C/EBPε Results in Highly Defective Myeloid Differentiation and Lack of Cytokine Response. (2010)
  • Author(s): Akagi T, Thoennissen NH, George A, Crooks G, Song JH, Okamoto R, Nowak D, Gombart AF, Koeffler HP.
  • Journal Title: PLoS ONE. 5 Pages: 15419-15419
  • NAID: 120002647977
  • Peer Reviewed
• [Journal Article] Genomic profiling of adult acute lymphoblastic leukemia (ALL) by single nucleotide polymorphism oligonucleotide microarray and comparison to pediatric ALL. (2010)
  • Author(s): Okamoto R, Ogawa S, Nowak D, Kawamata N, Akagi T, Kato M, Sanada M, Weiss T, Haferlach C, Dugas M, Ruckert C, Haferlach T, Koeffler HP.
  • Journal Title: Haematologica. 95 Pages: 1481-1488
  • Peer Reviewed
• [Journal Article] In vivo deficiency of both C/EBPβ and C/EBPε results in highly defective myeloid differentiation and lack of cytokine response. (2010)
  • Author(s): Akagi, T., et al
  • Journal Title: PLoS One Volume: 5
  • NAID: 120002647977
  • Peer Reviewed
• [Journal Article] Genomic profiling of adult acute lymphoblastic leukemia by single nucleotide polymorphism oligonucleotide microarray and comparison to pediatric ALL. (2010)
  • Author(s): Okamoto, R., et al
  • Journal Title: Haematologica Volume: 95 Pages: 1481-1488
  • Peer Reviewed
• [Journal Article] Dax1 Binds to Oct3/4 and Inhibits Its Transcriptional Activity in Embryonic Stem Cells (2009)
  • Author(s): Sun C, Nakatake Y, Akagi T, Ura H, Matsuda T, Nishiyama A, Koide H, Ko MS, Niwa H, Yokota T.
  • Journal Title: .Mol Cell Biol. 29 Pages: 4574-4583
  • Peer Reviewed
• [Journal Article] Chromosomal abnormalities and novel disease-related regions in progression from Barrett's esophagus to esophageal adenocarcinoma. (2009)
  • Author(s): Akagi T, Ito T, Kato M, Jin Z, Cheng Y, Kan T, Yamamoto G, Olaru A, Kawamata N, Boult J, Soukiasian HJ, Miller CW, Ogawa S, Meltzer SJ, Koeffler HP.
  • Journal Title: Int J Cancer. 125 Pages: 2349-2359
  • Peer Reviewed
• [Journal Article] Dax1 binds to Oct3/4 and inhibits its transcriptional activity in embryonic stem cells. (2009)
  • Author(s): Sun. C
  • Journal Title: Mol Cell Biol 29 Pages: 4574-4583
  • Peer Reviewed
• [Journal Article] Chromosomal abnormalities and novel disease-related regions in progression from Barrett's esophagus to esophageal adenocarcinoma. (2009)
  • Author(s): Akagi. T
  • Journal Title: Int J Cancer. 125 Pages: 2349-2359
  • Peer Reviewed
• [Presentation] 未分化なES細胞特異的に発現しているDax1とEsrrbの相互作用 (2010)
  • Author(s): 浦西洸介、赤木紀之、孫伝海、小出寛、横田崇
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県、神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] In vivo deficiency of both C/EBPbeta and C/EBP epsilon results in highly defective myeloid differentiation and lack of cytokine reponse. (2010)
  • Author(s): Tadayuki Akagi, Nils H.Thoennissen, Ann George, Gay Crooks, Jee Hoon Song, Ryoko Okamoto, Daniel Nowak, Carsten Muller-Tidow, Adrian F.Gombart, H.Phillip Koeffler.
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県、神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Nanog is essential for anchorage-independent growth of HT1080 fibrosarcoma cells. (2010)
  • Author(s): 田多祐喜, 赤木紀之, 横田崇, 小出寛.
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県、神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 未分化なES細胞特異的に発現しているDax1とEsrrbの相互作用 (2010)
  • Author(s): 浦西洸介, ら
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-09
• [Presentation] Redundant roles of C/EBP-beta and C/EBP-epsilon during myeloid development. (2009)
  • Author(s): 赤木紀之, Nils H.Thoennissen, Ann George, Gay Crooks, Jee Hoon Song, 岡本涼子, Daniel Nowak, Carsten Muller-Tidow, Adrian F.Gombart, H.Phillip Koeffler.
  • Organizer: 第32回分子生物学会年会
  • Place of Presentation: 神奈川県、パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] マウスES細胞の生存や分化には、Eedの適切な発現レベルが必要である (2009)
  • Author(s): 浦大樹, 赤木紀之, 小出寛, 横田崇
  • Organizer: 第32回分子生物学会年会
  • Place of Presentation: 神奈川県、パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] ヒト線維肉腫HT1080細胞の足場非依存性増殖はNanogによって促進される (2009)
  • Author(s): 田多祐喜, 赤木紀之, 横田崇, 小出寛
  • Organizer: 第32回分子生物学会年会
  • Place of Presentation: 神奈川県、パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] 転写因子C/EBPβとC/EBPεのダブルノックアウトマウスにおける造血器機能の解析 (2009)
  • Author(s): 赤木紀之, H.Phillip Koeffler.
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 神奈川県、パシフィコ横浜
  • Year and Date: 2009-10-03
• [Presentation] 転写因子C/EBPβとC/EBPεのグブルノックアウトマウスにおける造血器機能の解析 (2009)
  • Author(s): 赤木紀之
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-03
• [Presentation] Analysis of Hematopoietic Cells in C/EBPβ and C/EBPε Double Knockout Mice. (2009)
  • Author(s): Tadayuki Akagi, Nils H.Thoennissen, Gay Crooks, Adrian F.Gombart, H.Phillip Koeffler.
  • Organizer: The 7th Stem Cell Research Symposium
  • Place of Presentation: 東京都、泉ガーデンギャラリー
• [Presentation] Excess amount of Eed induces apoptosis of mouse ES cells. (2009)
  • Author(s): Hiroki Ura, Tadayuki Akagi, Hiroshi Koide, Takashi Yokota.
  • Organizer: The 7th Stem Cell Research Symposium
  • Place of Presentation: 東京都、泉ガーデンギャラリー