Differentiation of steroidogenic cells from stem cells.

• Project/Area Number: 21790269
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: University of Fukui
• Principal Investigator: YAZAWA Takashi University of Fukui, 医学部, 助教 (00334813)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 再生医学 / 幹細胞 / SF-1 / ステロイドホルモン / 間葉系幹細胞 / PGC-1α / 顆粒膜細胞

Research Abstract

In this study, I developed a method for differentiation of ES cells into steroidogenic cells by a tetracycline regulated gene expression system for SF-1 using ROSA-TET system. I also revealed that PGC-1α is important for the progesterone production in ovarian granulosa cells.

Research Products

• [Journal Article] Stem cell differentiation into steroidogenic cell lineages by NR5A family. (2011)
  • Author(s): Miyamoto, K., Yazawa, T., Mizutni, T., Imamichi, Y., Kawabe, S., Ju, Y., Umezawa, A.
  • Journal Title: Mol.Cell.Endocrinol. 336 Pages: 123-126
  • Peer Reviewed
• [Journal Article] Differentiation of mesenchymal stem cells and embryonic stem cells into steroidogenic cells using steroidogenic factor-1 and liver receptor homolog-1. (2011)
  • Author(s): Yazawa, T., Kawabe, S., Inaoka, Y., Okada, R., Mizutani, T., Imamichi, Y., Ju, Y., Yamazaki, Y., Usami, Y., Kuribayashi, M., Umezawa, A., Miyamoto, K.
  • Journal Title: Mol.Cell.Endocrinol. 336 Pages: 127-132
  • Peer Reviewed
• [Journal Article] High temperature causes masculinization of genetically female medaka by elevation of cortisol level. (2011)
  • Author(s): Hayashi, Y., Kobira, H., Yamaguchi, T., Shiraishi, E., Yazawa, T., Hirai, T., Kamei, Y., Kitano, T.
  • Journal Title: Mol.Reprod.Dev. 77(8) Pages: 679-686
  • Peer Reviewed
• [Journal Article] Stem cell differentiation into steroidogenic cell lineages by NR5A family. (2011)
  • Author(s): Miyamoto, K.
  • Journal Title: Mol.Cell.Endocrinol. Volume: 336 Pages: 123-126
  • Peer Reviewed
• [Journal Article] Differentiation of mesenchymal stem cells and embryonic stem cells into steroidogenic cells using steroidogenic factor-1 and liver receptor homolog-1. (2011)
  • Author(s): Yazawa, T.
  • Journal Title: Mol.Cell.Endocrinol. Volume: 336 Pages: 127-132
  • Peer Reviewed
• [Journal Article] 卵巣顆粒膜細胞における転写共役因子PGC-1αの役割 (2010)
  • Author(s): 矢澤隆志, 梅澤明弘, 宮本薫
  • Journal Title: 日本生殖内分泌学会雑誌 15 Pages: 29-34
• [Journal Article] Identification of a novel distal control region upstream of the human steroidogenic acute regulatory protein (StAR) gene that participates in SF-1-dependent chromatin architecture. (2010)
  • Author(s): Mizutani, T., Yazawa, T., Ju, Y., Imamichi, Y., Uesaka, M., Inaoka, Y., Matsuura, K., Kamiki, Y., Oki, M., Umezawa, A., Miyamoto, K.
  • Journal Title: J.Biol.Chem. 285(36) Pages: 28240-28251
  • Peer Reviewed
• [Journal Article] Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. (2010)
  • Author(s): Yamaguchi, T., Yoshinaga, N., Yazawa, T., Gen, K., Kitano, T.
  • Journal Title: Endocrinol. 151(8) Pages: 3900-3908
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha regulates progesterone production in ovarian granulosa cells with steroidogenic factor-1 and liver receptor homolog-1. (2010)
  • Author(s): Yazawa, T., Inaoka, Y., Okada, R., Mizutani, T., Yamazaki, Y., Usami, Y., Kuribayashi, M., Orisaka, M., Umezawa, A., Miyamoto, K.
  • Journal Title: Mol.Endocrinol. 24(3) Pages: 485-496
  • Peer Reviewed
• [Journal Article] 卵巣顆粒膜細胞における転写共役因子PGC-1αの役割 (2010)
  • Author(s): 矢澤隆志
  • Journal Title: 日本生殖内分泌学会雑 Volume: 15 Pages: 29-34
• [Journal Article] Identification of a novel distal control region upstream of the human steroidogenic acute regulatory protein (StAR) gene that participates in SF-1-dependent chromatin architecture. (2010)
  • Author(s): Mizutani, T.
  • Journal Title: J.Biol.Chem. Volume: 285(36) Pages: 28240-28251
  • Peer Reviewed
• [Journal Article] High temperature causes masculinization of genetically female medaka by elevation of cortisol level. (2010)
  • Author(s): Hayashi, Y.
  • Journal Title: Mol.Reprod.Dev. Volume: 77(8) Pages: 679-686
  • Peer Reviewed
• [Journal Article] Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. (2010)
  • Author(s): Yamaguchi, T.
  • Journal Title: Endocrinology Volume: 151(8) Pages: 3900-3908
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha regulates progesterone production in ovarian granulosa cells with steroidogenic factor-1 and liver receptor homolog-1. (2010)
  • Author(s): Yazawa, T.
  • Journal Title: Mol.Endocrinol. 24(3) Pages: 485-496
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞からのステロイド産生細胞 (2009)
  • Author(s): 矢澤隆志、梅澤明弘、宮本薫
  • Journal Title: 特集・再生医療の将来と産婦人科.産科と婦人科 76(10) Pages: 1189-1194
• [Journal Article] Liver Receptor Homolog-1 regulates the transcription of steroidogenic enzymes and induces the differentiation of mesenchymal stem cells into steroidogenic cells. (2009)
  • Author(s): Yazawa, T., Inaoka, Y., Mizutani, T., Kuribayashi, M., Umezawa, A., Miyamoto, K.
  • Journal Title: Endocrinology 150(8) Pages: 3885-3893
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞からのステロイド産生細胞 (2009)
  • Author(s): 矢澤隆志
  • Journal Title: 産科と婦人科 76(10) Pages: 1189-1194
• [Journal Article] Liver Receptor Homolog-1 regulates the transcription of steroidogenic enzymes and induces the differentiation of mesenchymal stem cells into steroidogenic cells. (2009)
  • Author(s): Yazawa, T.
  • Journal Title: Endocrinology 150(8) Pages: 3885-3893
  • Peer Reviewed
• [Presentation] 幹細胞を用いたステロイドホルモン産生機構の解明 (2011)
  • Author(s): 矢澤隆志
  • Organizer: ペプチド・ホルモン宮崎若手研究会
  • Place of Presentation: 宮崎市
  • Year and Date: 2011-03-04
• [Presentation] 卵巣顆粒膜細胞における転写共役因子・PGC-1αの機能 (2010)
  • Author(s): 矢澤隆志, 稲岡斉彦, 岡田令子, 水谷哲也, 山崎由希子, 宇佐美陽子, 栗林真悠, 梅澤明弘, 宮本薫
  • Organizer: 日本動物学会中部支部大会2010
  • Place of Presentation: 岐阜
  • Year and Date: 2010-07-25
• [Presentation] ヒトStAR遺伝子の新たな転写調節機構 (2010)
  • Author(s): 水谷哲也, 矢澤隆志, 具云峰, 今道力敬, 松村健大, 河邉真也, 松浦かおる, 上木康衣, 梅澤明弘, 宮本薫
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井
  • Year and Date: 2010-05-29
• [Presentation] PGC-1αはSF-1とLRH-1のコアクチベーターとしてプロジェステロン産生を促進する (2010)
  • Author(s): 矢澤隆志, 稲岡斉彦, 岡田令子, 河邉真也, 水谷哲也, 今道力敬, 山崎由希子, 宇佐美陽子, 栗林真悠, 梅澤明弘, 宮本薫
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会 BMB2010.
  • Place of Presentation: 神戸
• [Presentation] クロマチン構造変換を介したStARの新たな転写調節メカニズム (2010)
  • Author(s): 水谷哲也, 具云峰, 今道力敬, 松村健大, 矢澤隆志, 河邉真也, 菅野真史, 松浦かおる, 上木康衣, 梅澤明弘, 尾崎司, 南野直人, 宮本薫
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会 BMB2010.
  • Place of Presentation: 神戸
• [Presentation] 卵巣ステロイドホルモン合成に関連する遺伝子発現調節機構の新知見 (2010)
  • Author(s): 矢澤隆志
  • Organizer: 第15回日本生殖内分泌学会学術集会 卵巣機能調節における新知見
  • Place of Presentation: 千里
• [Presentation] 卵巣・顆粒膜細胞におけるアンドロジェンの作用 (2010)
  • Author(s): 矢澤隆志, 河邉真也, 稲岡斉彦, 岡田令子, 水谷哲也, 今道力敬, 山崎由希子, 宇佐美陽子, 栗林真悠, 梅澤明弘, 宮本薫
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
• [Presentation] Regulation of SF-1-mediated transcription of the human steroidogenic acute regulatory protein gene by chromation-loop formation. (2010)
  • Author(s): Mizutani, T., Yazawa, T., Ju, Y., Uesaka, M., Inaoka, Y., Imamichi, Y., Matsuura, K., Kamiki, Y., Umezawa, A., Miyamoto, K.
  • Organizer: The 92th Annual Meeting & Expo
  • Place of Presentation: San Diego.
• [Presentation] Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha regulates progesterone production in ovarian granulosa cells with steroidogenic factor-1 and liver receptor homolog-1. (2010)
  • Author(s): Yazawa, T., Umezawa, A., Miyamoto, K.
  • Organizer: The 92th Annual Meeting & Expo
  • Place of Presentation: San Diego.
• [Presentation] LRH-1 regulates the transcription of steroidogenic enzymes and induces the differentiation of mesenchymal stem cells into steroidogenic cells. (2010)
  • Author(s): Yazawa, T., Umezawa, A., Miyamoto, K.
  • Organizer: XIV Adrenal Cortex Conference and the Keith Parker Memorial Symposium
  • Place of Presentation: San Diego.
• [Presentation] Stem cell differentiation into steroidogenic cell lineages by NR5A family. (2010)
  • Author(s): Miyamoto, K., Mizutani, T., Yazawa, T.
  • Organizer: XIV Adrenal Cortex Conference and the Keith Parker Memorial Symposium.Adrenal growth and development.
  • Place of Presentation: San Diego.
• [Presentation] Identification of a novel enhancer region in the human steroidogenic acute regulatory protein (StAR) gene. (2010)
  • Author(s): Mizutani, T., Yazawa, T., Uesaka, M., Inaoka, Y., Ju, Y., Okada, R., Matsuura, K., Kamiki, Y., Umezawa, A., Miyamoto, K.
  • Organizer: 14th International Congress of Endocrinology.
  • Place of Presentation: Kyoto.
• [Presentation] PGC-1alpha regulates progesterone production in ovarian granulose cells with SF-1 and LRH-1. (2010)
  • Author(s): Yazawa, T., Miyamoto, K.
  • Organizer: 14th International Congress of Endocrinology.
  • Place of Presentation: Kyoto.
• [Presentation] LRH-1 regulates the transcription of steroidogenic enzymes and induces the differentiation of mesenchymal stem cells into steroidogenic cells. (2010)
  • Author(s): Yazawa, T.
  • Organizer: XIV Adrenal Cortex Conference and the Keith Parker Memorial Symposium
  • Place of Presentation: San Diego
• [Presentation] Stem cell differentiation into steroidogenic cell lineages by NR5A family. (2010)
  • Author(s): Miyamoto, K.
  • Organizer: XIV Adrenal Cortex Conference and the Keith Parker Memorial Symposium Adrenal growth and development
  • Place of Presentation: San Diego
• [Presentation] Regulation of SF-1-mediated transcription of the human steroidogenic acute regulatory protein gene by chromation-loop formation. (2010)
  • Author(s): Mizutani, T.
  • Organizer: The 92th Annual Meeting & Expo
  • Place of Presentation: San Diego
• [Presentation] Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha regulates progesterone production in ovarian granulosa cells with steroidogenic factor-1 and liver receptor homolog-1. (2010)
  • Author(s): Yazawa, T.
  • Organizer: The 92th Annual Meeting & Expo
  • Place of Presentation: San Diego
• [Presentation] 卵巣ステロイドホルモン合成に関連する遺伝子発現調節機構の新知見 (2010)
  • Author(s): 矢澤隆志
  • Organizer: 第15回日本生殖内分泌学会学術集会シンポジウム 卵巣機能調節における新知見
  • Place of Presentation: 千里
• [Presentation] クロマチン構造変換を介したStARの新たな転写調節メカニズム (2010)
  • Author(s): 水谷哲也
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会 BMB2010
  • Place of Presentation: 神戸
• [Presentation] PGC-1αはSF-1とLRH-1のコアクチベーターとしてプロジェステロン産生を促進する (2010)
  • Author(s): 矢澤隆志
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会 BMB2010
  • Place of Presentation: 神戸
• [Presentation] Identification of a novel enhancer region in the human steroidogenic acute regulatory protein (StAR) gene. (2010)
  • Author(s): Mizutani, T.
  • Organizer: 14^<th> International Congress of Endocrinology.
  • Place of Presentation: 京都
• [Presentation] PGC-lalpha regulates progesterone production in ovarian granulose cells with SF-1 and LRH-1. (2010)
  • Author(s): Yazawa, T.
  • Organizer: 14^<th> International Congress of Endocrinology.
  • Place of Presentation: 京都
• [Presentation] ヒトSteroidogenic Acute Regulatory Protein (StAR) 遺伝子における新たなSF-1/Ad4BP結合領域の同定 (2009)
  • Author(s): 水谷哲也, 矢澤隆志, 上坂美紀, 稲岡斉彦, 具云峰, 岡田令子, 松浦かおる, 上木康衣, 梅澤明弘, 宮本薫
  • Organizer: 第14回日本生殖内分泌学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-28
• [Presentation] 卵巣顆粒膜細胞における転写共役因子PGC-1αの機能 (2009)
  • Author(s): 矢澤隆志, 稲岡斉彦, 岡田令子, 水谷哲也, 山崎由希子, 宇佐美陽子, 栗林真悠, 梅澤明弘, 宮本薫
  • Organizer: 第14回日本生殖内分泌学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-28
• [Presentation] ヒトSteroidogenic Acute Regulatory Protein (StAR)遺伝子における新たなSF-1/Ad4BP結合領域の同定 (2009)
  • Author(s): 水谷哲也
  • Organizer: 第14回日本生殖内分泌学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-28
• [Presentation] ラット卵巣におけるゴナドトロピンによるP450 oxidoreductaseの発現調節とエストロゲン産生への影響 (2009)
  • Author(s): 稲岡斉彦, 矢澤隆志, 水谷哲也, 梅澤明弘, 宮本薫
  • Organizer: 本生化学会北陸支部第27回大会 要旨集 26, 2009
  • Place of Presentation: 福井
  • Year and Date: 2009-05-23
• [Presentation] PGC-1αのステロイドホルモン合成に対する作用 (2009)
  • Author(s): 矢澤隆志, 稲岡斉彦, 水谷哲也, 岡田令子, 山崎由希子, 栗林真悠, 梅澤明弘, 宮本薫
  • Organizer: 日本生化学会北陸支部第27回大会 要旨集 27,2009
  • Place of Presentation: 福井
  • Year and Date: 2009-05-23
• [Presentation] ラット卵巣におけるゴナドトロピンによるP450 Oxidoreductaseの発現調節とエストロゲン産生への影響 (2009)
  • Author(s): 稲岡斉彦
  • Organizer: 日本生化学会北陸支部第27回大会
  • Place of Presentation: 福井
  • Year and Date: 2009-05-23
• [Presentation] PGC-1αのステロイドホルモン合成に対する作用 (2009)
  • Author(s): 矢澤隆志
  • Organizer: 日本生化学会北陸支部第27回大会
  • Place of Presentation: 福井
  • Year and Date: 2009-05-23
• [Presentation] マウス生殖腺における魚類アンドロゲン・11-KT産生 (2009)
  • Author(s): 矢澤隆志, 宮本薫
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会(CompBiol2009)
  • Place of Presentation: 千里
• [Presentation] Identification of a novel enhancer region in the human steroidogenic acute regulatory protein (StAR) gene. (2009)
  • Author(s): 水谷哲也, 矢澤隆志, 上坂美紀, 稲岡斉彦, 具云峰, 岡田令子, 松浦かおる, 上木康衣, 梅澤明弘, 宮本薫
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] PGC-1αは卵巣顆粒膜細胞のプロジェステロン合成を亢進させる (2009)
  • Author(s): 矢澤隆志, 稲岡斉彦, 岡田令子, 水谷哲也, 山崎由希子, 宇佐美陽子, 栗林真悠, 梅澤明弘, 宮本薫
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
• [Presentation] 間葉系幹細胞からのステロイドホルモン産生細胞の作製 (2009)
  • Author(s): 矢澤隆志
  • Organizer: 第27回内分泌代謝学サマーセミナー.シンポジウム幹細胞研究の最前線 抄録集 30, 2009
  • Place of Presentation: 福井
• [Presentation] 転写共役因子PGC-1αの卵巣機能における役割 (2009)
  • Author(s): 矢澤隆志, 稲岡斉彦, 水谷哲也, 宮本薫
  • Organizer: 第82回日本内分泌学会学術総会公開シンポジウム4間脳下垂体性腺系の分子機構の新知識 日本内分泌学会雑誌 85(1), 213, 2009
  • Place of Presentation: 前橋
• [Presentation] ラット卵巣でのゴナドトロピンによるP450 oxidoreductaseの発現調節とエストロゲン産生に及ぼす効果 (2009)
  • Author(s): 稲岡斉彦, 矢澤隆志, 水谷哲也, 上坂美紀, 梅澤明弘, 宮本薫
  • Organizer: 第82回日本内分泌学会学術総会 日本内分泌学会雑誌 85(1), 348, 2009
  • Place of Presentation: 前橋
• [Presentation] 転写共役因子PGC-1αの卵巣機能における役割 (2009)
  • Author(s): 矢澤隆志
  • Organizer: 第82回日本内分泌学会学術総会 公開シンポジウム4間脳下垂体性腺系の分子機構の新知識
  • Place of Presentation: 前橋
• [Presentation] ラット卵巣でのゴナドトロピンによるP450 Oxidoreductaseの発現調節とエストロゲン産生に及ぼす効果 (2009)
  • Author(s): 稲岡斉彦
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 前橋
• [Presentation] 間葉系幹細胞からのステロイドホルモン産生細胞の作製 (2009)
  • Author(s): 矢澤隆志
  • Organizer: 第27回内分泌代謝学サマーセミナーシンポジウム 幹細胞研究の最前線
  • Place of Presentation: 福井
• [Presentation] PGC-1αは卵巣顆粒膜細胞のプロジェステゴン合成を充進させる (2009)
  • Author(s): 矢澤隆志
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡