Elucidation of mechanisms of generating non-cording RNA involved in tumorigenesis and virus proliferation.

• Project/Area Number: 21790280
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kochi University
• Principal Investigator: SAKAMOTO Shuji 高知大学, 教育研究部・医療学系, 助教 (80397546)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: microRNA / NF90-NF45 / 肝細胞がん / プロモーター解析 / トランスジェニックマウス / 筋組織 / ミトコンドリア空胞変性 / PGC-1 / がん / 筋形成 / ミトコンドリア / 空胞変性 / 翻訳 / 核内ミトコンドリア因子 / 核酸 / 癌 / 転写 / マイクロRNA

Research Abstract

1. We have proposed a novel tumorigenic model in which NF90-NF45 complex raised by E2F1 and 2 transactivators impairs the generation of miR-7 which is known to be an anti-oncomiR, resulting in a promotion of tumorigenesis in hepatocellular carcinoma.
2. We found that an overexpression of NF90 causes muscular atrophy accompanied with mitochondrial disruption via NF90-induced translational repression of nuclear-encoded mitochondria-related proteins such as PGC-1.

Research Products

• [Journal Article] Regulation of experimental autoimmune uveoretinitis by anti-Delta-like ligand 4 monoclonal antibody (2011)
  • Author(s): Ishida W, Fukuda K, Sakamoto S, Koyama N, Koyanagi A, Yagita H, Fukushima A
  • Journal Title: Invest Ophthalmol Vis sci Volume: 52(11) Pages: 8224-30
  • Peer Reviewed
• [Journal Article] microRNA生合成における負の制御 (2011)
  • Author(s): 坂本修士, 樋口琢磨, 戸高寛
  • Journal Title: The Lung perspective Volume: 19(3) Pages: 96-103
• [Journal Article] Regulation of T Cell Receptor Vg2 Gene Rearrangement by the Helix-Loop-Helix Protein, E2A (2011)
  • Author(s): Nozaki M, Wakae K, Tamaki N, Sakamoto S, Ohnishi K, Uejima T, Minato N, Yanagihara I, Agata Y
  • Journal Title: International Immunology Volume: 23(5) Pages: 297-305
  • Peer Reviewed
• [Journal Article] A Novel and comprehensive mouse model of human non-alcoholic steatohepatitis with the full range of dysmetabolic and histological abnomalities induced by gold-thioglucose and a high-fat diet (2011)
  • Author(s): Ogasawara M, Hirose A, Ono M, Aritake K, Nozaki Y, Takahashi M, Okamoto N, Sakamoto S, Iwasaki S, Asanuma T, Taniguchi T, Ueda Y, Onishi S, Saibara T, Oben JA
  • Journal Title: Liver International Volume: 31(4) Pages: 542-51
  • Peer Reviewed
• [Journal Article] Dynamic changes of microRNAs in the eye during the development of experimental autoimmune uveoretinitis (2011)
  • Author(s): Ishida W, Fukuda K, Higuchi T, Kajisako M, Sakamoto S, Fukushima A
  • Journal Title: Invest. Ophthalmol. Vis. Sci Volume: 52(1) Pages: 611-617
  • Peer Reviewed
• [Journal Article] Regulation of experimental autoimmune uveoretinitis by anti-Delta-like ligand 4 monoclonal antibody (2011)
  • Author(s): Ishida W., et al
  • Journal Title: Invest.Ophthalmol.Vis.Sci. Volume: 52(11) Pages: 8224-8230
  • Peer Reviewed
• [Journal Article] microRNA生合成における負の制御 (2011)
  • Author(s): 坂本修士
  • Journal Title: The LUNG perspective Volume: 19(3) Pages: 96-103
• [Journal Article] Regulation of T Cell Receptor Vg2 Gene Rearrangement by the Helix-Loop-Helix Protein, E2A. (2011)
  • Author(s): Nozaki M., et al
  • Journal Title: International Immunology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] A Novel and comprehensive mouse model of human non-alcoholic steatohepatitis with the full range of dysmetabolic and histological abnomalities induced by gold-thioglucose and a high-fat diet. (2011)
  • Author(s): Ogasawara M., et al
  • Journal Title: Liver International Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Dynamic changes of microRNAs in the eye during the development of experimental autoimmune uveoretinitis. (2011)
  • Author(s): Ishida W., et al
  • Journal Title: Invest.Ophthalmol.Vis.Sci. Volume: 52(1) Pages: 611-617
  • Peer Reviewed
• [Journal Article] Systemic overexpression of IFN-gamma and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis (2009)
  • Author(s): Ishida W, Tsuru E, Tominaga A, Miyazaki J, Higuchi T, Sakamoto S, Fukushima A
  • Journal Title: Br. J. Ophthalmol Volume: 93(12) Pages: 1680-1685
  • Peer Reviewed
• [Journal Article] The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway. (2009)
  • Author(s): Sakamoto S
  • Journal Title: Molecular and Cellular Biology 29 Pages: 3754-3769
  • Peer Reviewed
• [Journal Article] Systemic overexpression of IFN-gamma and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis. (2009)
  • Author(s): Ishida W
  • Journal Title: British Journal of Ophthalmology 93 Pages: 1680-1685
  • Peer Reviewed
• [Presentation] A novel regulatory mechanism of cellular energy balance by Nuclear Factor 90(NF90) (2011)
  • Author(s): Shuji Sakamoto, Takuma Higuchi, Yoshihiko Kakinuma, Shoko Kai, Eunsup Chi, Hiroshi Todaka, Keiko Morisawa, Takako Ujihara, Ken-ichi Yagyu, Atsuki Fukushima, Masayuki Tsuda, Taketoshi Taniguchi
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Nuclear Factor 45(NF45)による細胞増殖促進作用に関与するNF45複合体の同定 (2011)
  • Author(s): 戸高寛,樋口琢磨,森澤啓子,小野正文,玉置信行,波多野悦郎,氏原隆子,池恩秀,津田雅之,坂本修士,谷口武利
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 肝細胞癌のおけるNuclear Factor 90(NF90)の発現増加機構の解明 (2011)
  • Author(s): 樋口琢磨,戸高寛,森澤啓子,小野正文,西原利治,玉置信行,波多野悦郎,竹崎由佳,花崎和弘,津田雅之,坂本修士,谷口武利
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 遺伝的背景を維持したLewisラットおよびBrown Norwayラット骨髄由来の樹状細胞株の樹立 (2011)
  • Author(s): 森澤啓子,小味秀行,苫谷文子,近藤基樹,都留忍,戸高寛,樋口琢磨,尾畑恩秀,坂本修士,津田雅之,村上雅尚,大畑雅典,谷口武利
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Molecular Mechanisms of Muscular Abnormality in Transgenic Mice Overexpressing NF90, a dsRNA binding protein (2011)
  • Author(s): Shuji Sakamoto, Takuma Higuchi, Yoshihiko Kakinuma, Shoko Kai, Hiroshi Todaka, Ken-ichi Yagyu, Eunsup Obata, Keiko Morisawa, Atsuki Fukushima, Masayuki Tsuda, Taketoshi Taniguchi
  • Organizer: RNA 2011
  • Place of Presentation: Kyoto
• [Presentation] 発生段階特異的なTCRγ遺伝子再構成における転写抑制因子Gfi-1の役割 (2011)
  • Author(s): 若江亨祥、坂本修士、山下政克、中山俊憲、湊長博、縣保年
  • Organizer: KTCC 2011
  • Place of Presentation: 京都
• [Presentation] A nove regulatory mechanism of cellular energy balance by Nuclear Factor 90 (NF90) (2011)
  • Author(s): 坂本修士
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Nuclear Factor 45 (NF45)による細胞増殖促進作用に関与するNF45複合体の同定 (2011)
  • Author(s): 戸高寛
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] 肝細胞癌のおけるNuclear Factor 90 (NF90)の発現増加機構の解明 (2011)
  • Author(s): 樋口琢磨
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] 遺伝的背景を維持したLewisラットおよびBrown Norwayフット骨髄由来の樹状細胞株の樹立 (2011)
  • Author(s): 森澤啓子
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Molecular Mechanisms of Muscular Abnormality in Transgenic Mice Overexpressing NF90, a dsRNA binding protein (2011)
  • Author(s): 坂本修士
  • Organizer: RNA2011
  • Place of Presentation: 京都国際会館(京都)
• [Presentation] 発生段階特異的なTCRγ遺伝子再構成における転写抑制因子Gfi-1の役割 (2011)
  • Author(s): 若江亨祥
  • Organizer: KTCC 2011
  • Place of Presentation: 京都平安ホテル(京都)
• [Presentation] Overexpression of Nuclear Factor 90 (NF90) Triggers the morphological abnormality of mitochondoria in cardiac and skeletal muscles of Transgenic (Tg) mice. (2010)
  • Author(s): Sakamoto S., et al
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 肝細胞癌において発現上昇しているNF90遺伝子のプロモーター解析 (2010)
  • Author(s): 樋口琢磨
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] IL-27分泌型Dendritic-like cellを用いたコラーゲン誘導型関節炎抑制効果の検討 (2010)
  • Author(s): 戸高寛
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 肝細胞癌における二本鎖RNA結合タンパク質Nuclear Factor 90 (NF90)の発現機構の解析 (2010)
  • Author(s): 樋口琢磨
  • Organizer: 第75回 日本インターフェロン・サイトカイン学会学術集会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2010-06-25
• [Presentation] Lewis rat骨髄由来のIL-27発現型dendritic-like cell(DLC)の樹立とその応用 (2010)
  • Author(s): 戸高寛
  • Organizer: 第75回 日本インターフェロン・サイトカイン学会学術集会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2010-06-25
• [Presentation] Overexpression of Nuclear Factor 90(NF90) Triggers the morphological abnormality of mitochondoria in cardiac and skeletal muscles of Transgenic(Tg) mice (2010)
  • Author(s): Sakamoto S, Higuchi T, Kakinuma Y, Yagyu K, Todaka H, Fukushima A, Tsuda M, Taniguchi T
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸
• [Presentation] 肝細胞癌において発現上昇しているNF90遺伝子のプロモーター解析 (2010)
  • Author(s): 樋口琢磨,坂本修士,氏原隆子,矢生健一,竹崎由佳,上村直,花崎和弘,小野正文,西原利治,玉置信行,波多野悦郎,森澤啓子,戸高寛,甲斐翔子,津田雅之,谷口武利
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸
• [Presentation] IL-27分泌型Dendritic-like cellを用いたコラーゲン誘導型関節炎抑制効果の検討 (2010)
  • Author(s): 戸高寛,沈淵,近藤基樹,森澤啓子,樋口琢磨,坂本修士,津田雅之,福島敦樹,谷口武利
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸
• [Presentation] Lewis rat骨髄由来のIL-27発現型dendritic-like cell(DLC)の樹立とその応用 (2010)
  • Author(s): 樋口琢磨,坂本修士,氏原隆子,矢生健一,竹崎由佳,上村直,花崎和弘,小野正文,西原利治,玉置信行,波多野悦郎,森澤啓子,戸高寛,甲斐翔子,津田雅之,谷口武利
  • Organizer: 第75回日本インターフェロン・サイトカイン学会学術集会
  • Place of Presentation: 北九州
• [Presentation] 二本鎖RNA結合蛋白質NF90-NF45によるmiRNA生合成経路における負の制御 (2009)
  • Author(s): Sakamoto S, Higuchi T, Todaka H, Morisawa K, Aoki K, Taniguchi T, Agata Y
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
• [Presentation] 二本鎖RNA結合蛋白質NF90-NF45によるmiRNA生合成経路における負の制御. (2009)
  • Author(s): Sakamoto S
  • Organizer: 第11回 RNAミーティング
  • Place of Presentation: 朱鷺メッセ(新潟)