| Project/Area Number |
21790289
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
YAMASHITA Akio Yokohama City University, 医学研究科, 講師 (20405020)
|
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 細胞医化学 / 遺伝情報複製 / 転写装置 / 再編 / 制御 |
|---|
| Research Abstract |
I cloned genomic regions of VEGF, IL6, IL5, IFNbeta and IFNgamma. I constructed IFNbeta gene knockin vector for the insertion of BoxB sequence at downstream of termination codon. Next, I generate cell lines (HeLa TetOff, HeLaTetOnAdvanced, HCT116、BEAS-2B TetOff) which containing a single FRT recombination site. I also constructed the pTRE-Tight-FRT IL-6, IFNbeta, IL28B, and IL-5 full length genes.
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