Molecular mechanisms of neuronal development and maturation regulated by alteration of membrane potential using cerebellar granule cells

• Project/Area Number: 21790294
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: 公益財団法人大阪バイオサイエンス研究所 (2011); Osaka Bioscience Institute (2009-2010)
• Principal Investigator: OKAZAWA Makoto 公益財団法人大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (40414130)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経発達 / 小脳顆粒細胞 / 静止膜電位 / シグナル伝達 / 神経成熟 / パッチクランプ法

Research Abstract

The role of alteration of electrophysiological properties of neurons on the formation of neuronal network remains to be elucidated. Using cerebellar granule cells, I demonstrated the processe of dendritic maturation and the mechanisms of activity-dependent up-regulation of maturation genes.

Research Products

• [Journal Article] The Etv1/Er81 transcription factor orchestrates activity-dependent gene regulation in the terminal maturation program of cerebellar granule cells (2011)
  • Author(s): Abe H, Okazawa M, Nakanishi S
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 12497-12502
  • Peer Reviewed
• [Journal Article] The Etv1/Er81 transcription factor orchestrates activity-dependent gene regulation in the terminal maturation program of cerebellar granule cells (2011)
  • Author(s): H.Abe, M.Okazawa, S.Nakanishi
  • Journal Title: PNAS Volume: 108 Pages: 12-497
  • Peer Reviewed
• [Journal Article] Protein kinase G dynamically modulates TASK1-mediated leak K+currents in cholinergic neurons of the basal forebrain (2010)
  • Author(s): Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y
  • Journal Title: J Neurosci Volume: 30 Pages: 5677-5689
  • Peer Reviewed
• [Journal Article] Protein Kinase G Dynamically Modulates TASK1-Mediated Leak K^+ Currents in Cholinergic Neurons of the Basal Forebrain (2010)
  • Author(s): H.Toyoda, M.Saito, M.Okazawa, K.Hirano, H.Sato, H.Abe, K.Takada, K.Funabiki, M.Takada, T.Kaneko, Y.Kang
  • Journal Title: J.Neurosci. Volume: 30 Pages: 5677-5689
  • Peer Reviewed
• [Journal Article] Protein Kinase G Dynamically Modulates TASK1-Mediated Leak K^+ Currents in Cholinergic Neurons of the Basal Forebrain (2010)
  • Author(s): Hiroki Toyoda, Mitsuru Saito, Makoto Okazawa et.al.
  • Journal Title: Journal of Neuroscience Vol 30 Pages: 5677-5689
  • Peer Reviewed
• [Journal Article] Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism (2009)
  • Author(s): Nakatani J, Tamada K, Hatanaka F, Ise S, Ohta H, Inoue K, Tomonaga S, Watanabe Y, Chung YJ, Banerjee R, Iwamoto K, Kato T, Okazawa M, Yamauchi K, Tanda K, Takao K, Miyakawa T, Bradley A, Takumi T
  • Journal Title: Cell Volume: 137 Pages: 1235-1246
  • Peer Reviewed
• [Journal Article] Abnormal Behavior in a Chromosome- Engineered Mouse Model for Human 15q11-13 Duplication Seen in Autism (2009)
  • Author(s): Jin Nakatani, …, Makoto Okazawa et.al.
  • Journal Title: Cell Vol 137 Pages: 1235-1246
  • Peer Reviewed
• [Presentation] The Etv1/Er81 transcription factor regulates activity-dependent gene expression in the terminal maturation program of cerebellar granule cells (2011)
  • Author(s): Abe H, Okazawa M, Nakanishi S
  • Organizer: 第34回日本分子生物学会年回
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] The Etv1/Er81 transcription factor regulates activity-dependent gene expression in the terminal maturation program of cerebellar granule cells (2011)
  • Author(s): H.Abe, M.Okazawa, S.Nakanishi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] Bidirectional regulation of TASK1 channels expressed in HEK cells by modulation of PKG (2011)
  • Author(s): 豊田博紀,齋藤充,岡澤慎,平尾圭子,佐藤元,阿部遙,高田健治,船曳和雄,高田昌彦,金子武嗣,姜英男
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] (PKG) bidirectionally modulates TASK1 currents in PKG-loaded HEK 293 cells (2010)
  • Author(s): Toyoda H., Saito M., Okazawa M., Hirao K., Sato H., Abe H., Takada K., Funabiki K., Takada M., Kaneko T., Y. K Protein kinase G
  • Organizer: Neuroscience the Society for Neuroscience's 40th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-15
• [Presentation] A pivotal role of calcineurin signaling in membrane potential-regulated terminal maturation of cerebellar granule cells (2009)
  • Author(s): Okazawa M, Abe H, Kiwada T, Katsukawa M, Nakanishi S
  • Organizer: 第32回日本分子生物学会年回
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] A pivotal role of calcineurin signaling in membrane potential-regulated terminal maturation of cerebellar granule cells (2009)
  • Author(s): Makoto Okazawa et.al.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] Mechanism of subunit switching from NR2B to NR2C in mouse cerebellar granule cell cultures (2009)
  • Author(s): Iijima K, Abe H, Okazawa M, Moriyoshi K, Nakanishi S
  • Organizer: 第39回北米神経科学学会年会(Neuroscience 2009, SfN's 39th annual meeting)
  • Place of Presentation: McCormick Place(Chicago, Illinois, USA)
  • Year and Date: 2009-10-20
• [Presentation] Mechanism of subunit switching from NR2B to NR2C in mouse cerebellar ganule cell cultures (2009)
  • Author(s): Kouichirou Iijima, Haruka Abe, Makoto Okazawa et.al.
  • Organizer: 第39回北米神経科学学会年会(Neuroscience 2009, SfN's 39th annual meeting)
  • Place of Presentation: McCormick Place(Chicago, Illinois, USA)
  • Year and Date: 2009-10-20
• [Presentation] Membrane potential-regulated calcineurin signaling in maturation of cerebellar granule cells (2009)
  • Author(s): Okazawa M, Abe H, Kiwada T, Katsukawa M, Nakanishi S
  • Organizer: 第39回北米神経科学学会年会(Neuroscience 2009, SfN's 39th annual meeting)
  • Place of Presentation: McCormick Place(Chicago, Illinois, USA)
  • Year and Date: 2009-10-19
• [Presentation] Membrane potential-regulated calcineurin signaling in maturation of cerebellar grapule cells (2009)
  • Author(s): Makoto Okazawa et.al.
  • Organizer: 第39回北米神経科学学会年会(Neuroscience 2009, SfN's 39th annual meeting)
  • Place of Presentation: McCormick Place(Chicago, Illinois, USA)
  • Year and Date: 2009-10-19
• [Presentation] Mechanism of subunit switching from NR2B to NR2C in mouse cerebellar granule cell cultures (2009)
  • Author(s): Iijima K, Abe H, Okazawa M, Moriyoshi K, Nakanishi S
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Mechanism of subunit switching from NR2B to NR2C in mouse cerebellar granule cell cultures (2009)
  • Author(s): Kouichirou Iijima, Haruka Abe, Makoto Okazawa et.al.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Membrane potential-regulated maturation of cerebellar granule cells (2009)
  • Author(s): Okazawa M, Abe H, Kiwada T, Katsukawa M, Nakanishi S
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-16
• [Presentation] Membrane potential-regulated maturation of cerebellar granule cells (2009)
  • Author(s): Makoto Okazawa et.al.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-16