analysis of human amniotic membrane-derived cells related to hematopoiesis-supportive functions
| Project/Area Number |
21790297
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUDO Kazuhiro 独立行政法人理化学研究所, 細胞材料開発室, 協力研究員 (10392002)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 羊膜細胞 / 臍帯細胞 / 造血幹細胞 / 造血支持能 / 共培養 / 共移植 / ヒト臍帯血CD34陽性細胞 / ヒト臍帯由来細胞 / ヒト羊膜由来細胞 / 長期共培養 / サイトカイン産生 / 臍帯血CD34陽性細胞 / 移植 / サイトカイン / 羊膜由来細胞 / 臍帯血 / CD34陽性細胞 |
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| Research Abstract |
Co-culture of cord blood CD34^+cells with human amniotic membrane(HAM)-or human umbilical cord(HUC)-derived cells caused increase of CD45^+hematopoietic cells. however, percentage of CD34^+cells was decreased with time of co-culture. Colony-forming cells(CFCs) were maintained in co-culture at least for 5 weeks. The chimerisms of human CD45^+cells in NOD/SCID mice were significantly higher when HAM-or HUC-derived cells were co-transplanted with CD34+cells than when CD34^+cells were transplanted alone. Those results strongly suggest that HAM-and HUC-derived cells have hematopoiesis-supportive functions.
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Report
(4 results)
Research Products
(2 results)
