Dysfunction of the chromatin remodeling SWI/SNF complex effects the gastric tumorigenesis and gastrointestinal differentiation.

• Project/Area Number: 21790306
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAMICHI Nobutake The University of Tokyo, 医学部附属病院, 助教 (30463897)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 胃癌 / 腸上皮化生 / エピジェネティクス / 消化管分化 / Cdx2 / SWI / SNF複合体 / Brm / miRNA / SWI/SNF複合体 / Cathepsin E

Research Abstract

The histologic type of gastric cancer is quite important for prediction of the patient's prognosis, evaluating the risk of invasion and/or metastasis, selection of the anti-tumor therapy, etc. Based on our recent study showing a correlation between Brm-deficiency and undifferentiated status of gastric cancer, we analyzed the expression of villin gene and found that Brm-type chromatin-remodeling SWI/SNF complex cooperatively regulates villin expression with Cdx2. Brm-deficiency in gastric canceration should influence expression of many gastrointestinal genes regulated by Cdx2, a transcription factor essential for intestinal development and homeostasis. It is strongly suggested that the correlation between Brm-deficiency and various histologic features of gastric cancer is based on the interaction with Cdx2 and the Brm-type SWI/SNF complex.

Research Products

• [Journal Article] Long-term outcomes of endoscopic submucosal dissection for colorectal epithelial neoplasms. (2010)
  • Author(s): Niimi K, Fujishiro M, Kodashima S, Goto O, Ono S, Hirano K, Minatsuki C, Yamamichi N, Koike K.
  • Journal Title: Endoscopy. 42 Pages: 723-729
  • Peer Reviewed
• [Journal Article] A second-look endoscopy after endoscopic submucosal dissection for gastric epithelial neoplasm may be unnecessary : a retrospective analysis of postendoscopic submucosal dissection bleeding. (2010)
  • Author(s): Goto O, Fujishiro M, Kodashima S, Ono S, Niimi K, Hirano K, Yamamichi N, Koike K.
  • Journal Title: Gastrointest Endosc. 71 Pages: 241-248
  • Peer Reviewed
• [Journal Article] A second-look endoscopy after endoscopic submucosal dissection for gastric epithelial neoplasm may be unnecessary : a retrospective analysis of postendoscopic submucosal dissection bleeding.. (2010)
  • Author(s): Goto O, Fujishiro M, Kodashima S, Ono S, Niimi K, Hirano K, Yamamichi N, Koike K.
  • Journal Title: Gastrointestinal Endoscopy. Volume: 71 Pages: 241-248
  • Peer Reviewed
• [Journal Article] Long-term outcomes of endoscopic submucosal dissection for colorectal epithelial neoplasms. (2010)
  • Author(s): Niimi K, Fujishiro M, Kodashima S, Goto O, Ono S, Hirano K, Minatsuki C, Yamamichi N, Koike K.
  • Journal Title: Endoscopy. Volume: 42 Pages: 723-729
  • Peer Reviewed
• [Journal Article] Long-term outcomes of endoscopic submucosal dissection for superficial esophageal squamous cell neoplasms. (2009)
  • Author(s): Ono S, Fujishiro M, Niimi K, Goto O, Kodashima S, Yamamichi N, Omata M.
  • Journal Title: Gastrointest Endosc. 70 Pages: 860-866
  • Peer Reviewed
• [Journal Article] Loss of the Brm-type SWI/SNF chromatin remodeling complex is a strong barrier to the Tat-independent transcriptional elongation of human immunodeficiency virus type 1 transcripts. (2009)
  • Author(s): Mizutani T, Ishizaka A, Tomizawa M, Okazaki T, Yamamichi N, Kawana-Tachikawa A, Iwamoto A, Iba H.
  • Journal Title: J Virol. 83 Pages: 11569-11580
  • Peer Reviewed
• [Journal Article] Locked nucleic acid in situ hybridization analysis of miR-21 expression during colorectal cancer development. (2009)
  • Author(s): Yamamichi N, Shimomura R, Inada K, Sakurai K, Haraguchi T, Ozaki Y, Fujita S, Mizutani T, Furukawa C, Fujishiro M, Ichinose M, Shiogama K, Tsutsumi Y, Omata M, Iba H.
  • Journal Title: Clin Cancer Res. 15 Pages: 4009-4016
  • Peer Reviewed
• [Journal Article] Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells. (2009)
  • Author(s): Yamamichi N, Inada K, Furukawa C, Sakurai K, Tando T, Ishizaka A, Haraguchi T, Mizutani T, Fujishiro M, Shimomura R, Oka M, Ichinose M, Tsutsumi Y, Omata M, Iba H.
  • Journal Title: Exp Cell Res. 315 Pages: 1779-1789
  • Peer Reviewed
• [Journal Article] Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells. (2009)
  • Author(s): Yamamichi N, Inada K, Furukawa C, Sakurai K, Tando T, Ishizaka A, Haraguchi T, Mizutani T, Fujishiro M, Shimomura R, Oka M, Ichinose M, Tsutsumi Y, Omata M, Iba H.
  • Journal Title: Experimental Cell Research Volume: 315 Pages: 1779-1789
  • Peer Reviewed
• [Journal Article] Locked nucleic acid in situ hybridization a nalysis of miR-21 expression during colorectal cancer development. (2009)
  • Author(s): Yamamichi N, Shimomura R, Inada K, Sakurai K, Haraguchi T, Ozaki Y, Fujita S, Mizutani T, Furukawa C, Fujishiro M, Ichinose M, Shiogama K, Tsutsumi Y, Omata M, Iba H.
  • Journal Title: Clinical Cancer Research Volume: 15 Pages: 4009-4016
  • Peer Reviewed
• [Journal Article] Loss of the Brm-type SWI/SNF chromatin remodeling complex is a strong barrier to the Tat-independent transcriptional elongation of human immunodeficiency virus type 1 transcripts. (2009)
  • Author(s): Mizutani T, Ishizaka A, Tomizawa M, Okazaki T, Yamamichi N, Kawana-Tachikawa A, Iwamoto A, Iba H.
  • Journal Title: Journal of Virology Volume: 83 Pages: 11569-11580
  • Peer Reviewed
• [Presentation] Cdx2とSWI/SNF複合体による腸上皮化生進展の分子機構-villin遺伝子に着目して (2010)
  • Organizer: 第9回消化器病フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2010-11-20
• [Presentation] Cdx2とSWI/SNF複合体による腸上皮化生進展の分子機構-villin遺伝子に着目して (2010)
  • Author(s): 山道信毅、稲田健一、小田島慎也、藤城光弘、一瀬雅夫、小池和彦、伊庭英夫
  • Organizer: 第9回消化器病フォーラム
  • Place of Presentation: 東京(品川プリンスホテル)
  • Year and Date: 2010-11-20
• [Presentation] クロマチン構造変換因子SWI/SNF複合体の胃癌における発現異常 触媒サブユニットBrmの発現欠失、組織型・分化マーカーとの関係 (2009)
  • Organizer: JDDW 2009
  • Place of Presentation: 京都
  • Year and Date: 2009-10-13
• [Presentation] クロマチン構造変換因子SWI/SNF複合体の胃癌における発現異常:触媒サブユニットBrmの発現欠失、組織型・分化マーカーとの関係 (2009)
  • Author(s): 山道信毅、稲田健一、一瀬雅夫、藤城光弘、小俣政男、伊庭英夫
  • Organizer: JDDW2009
  • Place of Presentation: 国立京都国際会議場
  • Year and Date: 2009-10-13
• [Remarks] 特になし