Biomarker Analysis from Human Age-Related Macular Degeneration using Imaging Mass Spectrometry

• Project/Area Number: 21790315
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: HAYASAKA Takahiro Hamamatsu University School of Medicine, 分子イメージング先端研究センター, 特任助教 (90415927)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 網膜 / 加齢黄斑変性症 / 質量分析 / イメージング / バイオマーカー / 綱膜

Research Abstract

In this study we tried to detect biomarkers of human age-related macular degeneration (ARMD) using imaging mass spectrometry (IMS). The optimization of conventional matrices onto the surface of biological section and the application of metal core nanoparticles as a matrix enabled us to detect several lipids and fatty acids by IMS analysis. We revealed the specific biomarker from ARMD compared with normal tissue section.

Research Products

• [Journal Article] Imaging Mass Spectrometry with Silver Nanoparticles Reveals the Distribution of Fatty Acids in Mouse Retinal Sections (2010)
  • Author(s): Hayasaka T, Goto-Inoue N, Zaima N, Shrivas K, Kashiwagi Y, Yamamoto M, Nakamoto M, Setou M.
  • Journal Title: 21(8) Pages: 1446-1454
  • Peer Reviewed
• [Journal Article] Comparison of Phospholipid Molecular Species between Terminal and Stem Villi of Human Term (2010)
  • Author(s): Kobayashi Y, Hayasaka T, Setou M, Itoh H, Kanayama N.
  • Journal Title: Placenta 31(3) Pages: 245-248
  • Peer Reviewed
• [Journal Article] Imaging Mass Spectrometry with Silver Nanoparticles Reveals the Distribution of Fatty Acids in Mouse Retinal Sections. (2010)
  • Author(s): Hayasaka T, Goto-Inoue N, Zaima N, Shrivas K, Kashiwagi Y, Yamamoto M, Nakamoto M, Seton M.
  • Journal Title: Journal of American Society for Mass Spectrometry Volume: 21(8) Pages: 1446-1454
  • Peer Reviewed
• [Journal Article] Comparison of Phospholipid Molecular Species between Terminal and Stem Villi of Human Term. (2010)
  • Author(s): Kobayashi Y^*, Hayasaka T^*, Setou M, Itoh H, Kanayama N.^*First two authors equally contributed to the study.
  • Journal Title: Placenta 31(3) Pages: 245-248
  • Peer Reviewed
• [Journal Article] Organ-Specific Distributions of Lysophosphatidylcholine and Triacylglycerol in Mouse Embryo (2009)
  • Author(s): Hayasaka T, Goto-Inoue N, Zaima N, Kimura Y, Setou M.
  • Journal Title: Lipids 44(9) Pages: 837-848
  • Peer Reviewed
• [Journal Article] Organ-Specific Distributions of Lysophosphatidylcholine and Triacylglycerol in Mouse Embryo. (2009)
  • Author(s): Hayasaka T, Goto-Inoue N, Zaima N, Kimura Y, Setou M.
  • Journal Title: Lipids 44(9) Pages: 837-848
  • Peer Reviewed
• [Journal Article] Development of imaging mass spectrometry (IMS) dataset extractor software, IMS Convolution
  • Author(s): Hayasaka T, Goto-Inoue N, Ushijima M, Yao I, Yuba-Kubo A, Wakui M, Kajihara S, Matsuura M, Setou M.
  • Journal Title: Analytical and Bioanalytical Chemistry in press
  • Peer Reviewed
• [Journal Article] Development of imaging mass spectrometry (IMS) dataset extractor software, IMS Convolution.
  • Author(s): Hayasaka T, Goto-Inoue N, Ushijima M, Yao I, Yuba-Kubo A, Wakui M, Kajihara S, Matsuura M, Setou M.
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: (in press)
  • Peer Reviewed
• [Presentation] 質量顕微鏡法を用いた分子イメージング (2010)
  • Author(s): 早坂孝宏
  • Organizer: 第125回質量分析学会関西談話会
  • Place of Presentation: 神戸薬科大学
  • Year and Date: 2010-09-25
• [Presentation] 質量顕微鏡を用いた分子イメージング (2010)
  • Author(s): 早坂孝宏
  • Organizer: 第125回質量分析学会関西談話会
  • Place of Presentation: 神戸薬科大学
  • Year and Date: 2010-09-25
• [Presentation] 質量顕微鏡法へのナノ粒子の応用 (2010)
  • Author(s): 早坂孝宏、井上菜穂子、財満信宏、Kamlesh Shrivas、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-22
• [Presentation] 銀ナノ粒子を用いた質量分析イメージングによる脂肪酸の可視化 (2010)
  • Author(s): 早坂孝宏、井上菜穂子、財満信宏、Kamlesh Shrivas、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 日本油化学会第49回年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-17
• [Presentation] MALDI-TOF MSを用いた質量顕微鏡法 (2010)
  • Author(s): 早坂孝宏、瀬藤光利
  • Organizer: マイクロビームアナリシス第141委員会第141回研究会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2010-08-30
• [Presentation] MALDI-TOF MSを用いた質量顕微鏡法 (2010)
  • Author(s): 早坂孝宏、瀬藤光利
  • Organizer: マイクロビームアナリシス第141委員会 第141回研究会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2010-08-30
• [Presentation] 金属ナノ粒子を用いた生体試料の質量分析イメージング (2010)
  • Author(s): 早坂孝宏、瀬藤光利
  • Organizer: 第2回ナノインク懇話会
  • Place of Presentation: 大阪市立工業研究所
  • Year and Date: 2010-08-27
• [Presentation] 銀ナノ微粒子を用いたマウス網膜の質量分析イメージング (2010)
  • Author(s): 早坂孝宏、井上菜穂子、財満信宏、Kamlesh Shrivas、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 第58回質量分析総合討論会,第1回アジア・オセアニア質量分析会議
  • Place of Presentation: つくば
  • Year and Date: 2010-06-16
• [Presentation] 銀ナノ微粒子を用いたマウス網膜の質量分析イメージング (2010)
  • Author(s): 早坂孝宏、井上菜穂子、財満信宏、Kamlesh Shrivas、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 第58回質量分析総合討論会,第1回アジア￥オセアニア質量分析会議
  • Place of Presentation: つくば
  • Year and Date: 2010-06-16
• [Presentation] 分子イメージングによる形態学 (2010)
  • Author(s): 早坂孝宏、財満信宏、池上浩司、小西慶幸、瀬藤光利
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手医科大学
  • Year and Date: 2010-03-28
• [Presentation] Imaging mass spectrometry revealed the tissue-specific distributions of lysophosphatidylcholine and triacylglycerol in mouse embryonic sections (2009)
  • Author(s): Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Mitsutoshi Setou
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] MSイメージングを用いたマウス胎児におけるLPC及びTAGの解析 (2009)
  • Author(s): 早坂孝宏、井上菜穂子、財満信宏、瀬藤光利
  • Organizer: 第57回質量分析総合討論会
  • Place of Presentation: つくば
  • Year and Date: 2009-05-13
• [Book] Method of Operating AXIMA-QIT as Imaging Instrument Imaging Mass Spectrometry : Protocols for Mass Microscopy Chapter 8 (2010)
  • Author(s): Takahiro Hayasaka
  • Total Pages: 15
• [Book] Imaging Mass Spectrometry : Protocols for Mass Microscopy(Chapter 8 : Method of Operating AXIMA-QIT as Imaging Instrument) (2010)
  • Author(s): Takahiro Hayasaka
  • Total Pages: 15
• [Remarks] ホームページ等
  • URL: http://www.hama-med.ac.jp/mt/setou/ja/
• [Remarks]
  • URL: http://www.hama-med.ac.jp/mt/setou/ja/
• [Remarks]
  • URL: http://www.hama-med.ac.jp/university/chair/setou/ja/list.html