Analysis of apolipoprotein CIII function with transgenic rabbit

• Project/Area Number: 21790322
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Saga University
• Principal Investigator: NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 動脈硬化 / 遺伝子組換え / 病態モデル / ウサギ

Research Abstract

We attempted to create a transgenic rabbit as a tool to analyze the function of apolipoprotein(apo) CIII on lipid metabolism. 2727 ova were microinjected with human apoCIII expression DNA construct. 2276 embryos were transferred into 87 recipient rabbits, and 24 offspring were born. As a result of PCR analysis, no integration of human apoCIII gene was recognized in the offspring.