Analysis of a membrane bound transcription factor OASIS in osteogenesis

• Project/Area Number: 21790323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: University of Miyazaki
• Principal Investigator: MURAKAMI Tomohiko University of Miyazaki, 医学部, 助教 (50510723)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 小胞体 / 骨形成 / コラーゲン / 分泌 / 成長 / I型コラーゲン

Research Abstract

To assess the role of OASIS in vivo, we generated OASIS deficient mice. The mice exhibited osteopenia and growth retardation. Histological, and molecular and cellular biological analyses revealed that OASIS is involved in bone formation through transcription of type I collagen and secretion of bone matrix proteins. In contrast, the growth retardation in OASIS deficient mice were not improved by OASIS introduction in osteoblasts, indicating that the growth retardation is caused by the decrease in growth hormone and IGF-1 levels in OASIS deficient mice.

Research Products

• [Journal Article] Distinct mechanisms are responsible for osteopenia and growth retardation in OASIS-deficient mice. (2011)
  • Author(s): Murakami T, Hino S-I, Nishimura R, Yoneda T, Wanaka A, Imaizumi K.
  • Journal Title: Bone 48(3) Pages: 514-523
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum stress response mediated by the PERK-eIF2α-ATF4 pathway is involved in osteoblast differentiation induced by BMP2. (2011)
  • Author(s): Saito A, Ochiai K, Kondo S, Tsumagari K, Murakami T, Cavener, DR, Imaizumi K.
  • Journal Title: Journal of Biological Chemistry 286(6) Pages: 4809-4818
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum stress response mediated by the PERK-eIF2α-ATF4 pathway is involved in osteoblast differentiation induced by BMP2. (2011)
  • Author(s): Saito A, et al
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 4809-4818
  • Peer Reviewed
• [Journal Article] Effects of the bisphosphonate risedronate on osteopenia in OASIS-deficient mice. (2010)
  • Author(s): Sekiya H, Murakami T, Saito A, Hino S-I, Tsumagari K, Ochiai K, Imaizumi K.
  • Journal Title: Journal of Bone and Mineral Metabolism 28(4) Pages: 384-394
  • NAID: 10027208068
  • Peer Reviewed
• [Journal Article] Distinct mechanisms are responsible for osteopenia and growth retardation in OASIS-deficient mice (2010)
  • Author(s): Murakami T, et al
  • Journal Title: Bone Volume: 48 Pages: 514-523
  • Peer Reviewed
• [Journal Article] Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation. (2009)
  • Author(s): Murakami T, Saito A, Hino S-I, Kondo S, Kanemoto S, Chihara K, Sekiya H, Tsumagari K, Ochiai K, Yoshinaga K, Saitoh M, Nishimura R, Yoneda T, Kou I, Furuichi T, Ikegawa S, Ikawa M, Okabe M, Wanaka A, Imaizumi K.
  • Journal Title: Nature Cell Biology 11(10) Pages: 1205-1211
  • Peer Reviewed
• [Journal Article] Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. (2009)
  • Author(s): Saito A, Hino S-I, Murakami T, Kanemoto S, Kondo S, Saitoh M, Nishimura R, Yoneda T, Furuichi T, Ikegawa S, Ikawa M, Okabe M, Imaizumi K.
  • Journal Title: Nature Cell Biology 11(10) Pages: 1197-1204
  • Peer Reviewed
• [Journal Article] Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation (2009)
  • Author(s): Murakami T, et al
  • Journal Title: Nature Cell Biology 11 Pages: 1205-1211
  • Peer Reviewed
• [Journal Article] Regulation of ER stress response by BBF2H7-mediated Sec23a pathway is essential for chondrogenesis (2009)
  • Author(s): Saito A, et al
  • Journal Title: Nature Cell Biology 11 Pages: 1197-1204
  • Peer Reviewed
• [Journal Article] Effects of the bisphosphonate risedronate on osteopenia in OASIS-deficient mice (2009)
  • Author(s): Sekiya H, et al
  • Journal Title: Journal of Bone and Mineral Metabolism
  • Peer Reviewed
• [Presentation] 小胞体ストレスセンサーOASIS欠損マウスにおける骨形成不全と成長抑制 (2010)
  • Author(s): 村上智彦、西村理行、米田俊之、今泉和則
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] OASIS, an endoplasmic reticulum stress transducer, is involved in bone formation. (2010)
  • Author(s): Murakami T, Nishimura R, Yoneda T, Ikegawa S, Imaizumi K.
  • Organizer: Experimental Biology 2010(2010 American Society for Biochemistry and Molecular Biology(ASBMB)Annual Meeting)
  • Place of Presentation: Anaheim, CA, USA.
• [Presentation] 小胞体ストレスセンサーOASISファミリーの活性化機構と生体機能調節 (2010)
  • Author(s): 日野真一郎、齋藤敦、村上智彦、津曲健志、落合希実子、今泉和則
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 小胞体ストレスセンサーOASIS欠損マウスにおける骨形成不全と成長抑制 (2010)
  • Author(s): 村上智彦, 他
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] OASIS, an endoplasmic reticulum stress transducer, is involved in bone formation (2010)
  • Author(s): Murakami T, et al
  • Organizer: ASBMB Annual meeting
  • Place of Presentation: Anaheim, USA
• [Presentation] 小胞体ストレスセンサーOASISの骨形成における新機能 (2009)
  • Author(s): 村上智彦、西村理行、米田俊之、池川志郎、和中明生、今泉和則
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 小胞体ストレスセンサーOASISによる骨格形成制御 (2009)
  • Author(s): 村上智彦
  • Organizer: 特定領域研究「タンパク質の社会」2009年度班会議
  • Place of Presentation: 伊賀
• [Presentation] 小胞体膜貫通型転写因子OASISと骨形成 (2009)
  • Author(s): 村上智彦、西村理行、米田俊之、古市達哉、和中明生、今泉和則
  • Organizer: 第27回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 小胞体ストレスセンサーOASISによる骨形成制御 (2009)
  • Author(s): 村上智彦、西村理行、米田俊之、池川志郎、和中明生、今泉和則
  • Organizer: 日本分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
• [Presentation] 小胞体膜貫通型転写因子OASISと骨形成 (2009)
  • Author(s): 村上智彦, 他
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 小胞体ストレスセンサーOASISの骨形成における新機能 (2009)
  • Author(s): 村上智彦, 他
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Book] 実験医学 28(4)(小胞体ストレスセンサーによる骨軟骨形成制御) (2010)
  • Author(s): 今泉和則、村上智彦、齋藤敦
• [Book] 実験医学 小胞体ストレスセンサーによる骨軟骨形成制御 (2010)
  • Author(s): 今泉和則、村上智彦、齋藤敦
  • Total Pages: 6
  • Publisher: 羊土社
• [Book] 実験医学 27(4)(骨代謝のダイナミクスにおける小胞体ストレス応答の役割) (2009)
  • Author(s): 今泉和則、村上智彦