| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Pulmonary hypertension (PH) is the major cause of death in patients with mixed connective tissue disease (MCTD). However, pathogenic mechanism of PH in MCTD is still unclear. Since pulmonary micro vascular endothelial cell injury is the first change on the development of PH, I focused on one of the serum factors, anti-endothelial cell antibodies (AECA), in this study. For identification of targets of AECA, two-dimensional electrophoresis, western blot analysis, and mass spectrometry were performed. I also constructed cDNA library of pulmonary micro vascular endothelial cell and performed serological identification of antigens by recombinant expression cloning (SEREX). As these results, possible antigens, including already-known antigens, were identified.
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