Roles of HES1 and DEC2, newly identified transcriptional targets of GLI1, in pancreatic carcinogenesis.

• Project/Area Number: 21790370
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: Aichi Medical University
• Principal Investigator: INAGUMA Shingo Aichi Medical University, 医学部, 講師 (80410786)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 分子病理 / 膵臓癌 / シグナル伝達 / ヘッジホッグ / 転写因子 / GLI1

Research Abstract

We identified DEC2 as one of the direct transcriptional targets of GLI1, a downstream transcription factor of Hedgehog signaling pathway, via CACCC-box-like elements localized between -1126 and -1117 in the DEC2 promoter. We also showed that one of the mismatch repair gene MLH1 was down-regulated by GLI1-induced DEC2. We propose the possibility that Shh-GLI1-DEC2 pathway represses MLH1 expression and could enhance gene alteration and genomic instability in pancreatic carcinogenesis.

Research Products

• [Journal Article] GLI1 facilitates the migration and invasion of pancreatic cancer cells through MUC5AC-mediated attenuation of E-cadherin (2011)
  • Author(s): Shingo Inaguma, Kenji Kasai, Hiroshi Ikeda
  • Journal Title: Oncogene 30(6) Pages: 714-723
  • Peer Reviewed
• [Journal Article] GLI1 facilitates the migration and invasion of pancreatic cancer cells through MUC5AC-mediated attenuation of E-cadherin. (2011)
  • Author(s): Inaguma S., et al.
  • Journal Title: Oncogene Volume: 30巻 Pages: 714-723
  • Peer Reviewed
• [Presentation] GLI1はMUC5ACの発現誘導によってE-cadherinによる細胞間接着を阻害し膵癌細胞の遊走・浸潤を促進する (2010)
  • Author(s): 稲熊真悟、笠井謙次、池田洋
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 新宿区
  • Year and Date: 2010-04-29
• [Presentation] GLI1 induces the expression of bHLH type of transcriptional repressor DEC2 in pancreatic cancer cells (2010)
  • Author(s): Shingo Inaguma, Kenji Kasai, Hiroshi Ikeda
  • Organizer: 101st Annual meeting of American Association for Cancer Research
  • Place of Presentation: Washington, DC
  • Year and Date: 2010-04-20
• [Presentation] GLI1 induces the expression of bHLH type of transcriptional repressor DEC2 in pancreatic cancer cells. (2010)
  • Author(s): Inaguma S., et al.
  • Organizer: American Association for Cancer Research
  • Place of Presentation: Walter E.Washington Convention Center (Washington, DC)
  • Year and Date: 2010-04-20
• [Presentation] GLI1はgel forming mucin MUC5ACの発現上昇によって膵癌細胞の浸潤・遊走能を亢進させる (2009)
  • Author(s): 稲熊真悟、笠井謙次、池田洋
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-01
• [Presentation] 膵癌細胞においてGLI1はbHLH型転写抑制因子DEC2の発現を直接誘導しMLH1を介した遺伝子不安定制に寄与する (2009)
  • Author(s): 稲熊真悟、笠井謙次、米山亜紀子、池田洋
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2009-05-03
• [Presentation] 膵臓癌およびPancreatic Intraepithelial Neoplasia(PanIN)におけるSIL発現状況の免疫組織学的検討 (2009)
  • Author(s): 米山亜紀子、笠井謙次、稲熊真悟、池田洋
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2009-05-03
• [Remarks] ホームページ
  • URL: http://www.aichi-med-u.ac.jp/univ/medic/lecture2/10.html
• [Remarks]
  • URL: http://www.aichi-med-u.ac.jp/