Analysis on the roles of DOT1L histone methyltransferase in spermatogenesis

Research Project

Project/Area Number	21790375
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Experimental pathology
Research Institution	Kyoto University
Principal Investigator	MAKINO Yoshinori Kyoto University, 生命科学系キャリアパス形成ユニット, 研究員 (60431334)
Research Collaborator	OKADA Yuki 京都大学, 生命科学系キャリアパス形成ユニット, 助教 (60546430)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	エピジェネティクス / 精子形成 / ヒストンメチル化酵素
Research Abstract	The interaction between DOT1L H3K79 methyltransferase and physiological phenomenon in mammals remains to be clarified, though the functions of DOT1L in biochemistry and molecular biology have been well investigated. In this study we demonstrated mDot1l is essential for spermatogenesis by analysis of male germline specific mDot1l knockout mice and other methods. From this study we obtained a basic evidence to lead us to reveal a new function of DOT1L as well as clarified a role of a histone methyltransferase in spermatogenesis.