Alteration of microRNA expression and its role in bladder carcinogenesis

• Project/Area Number: 21790391
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Osaka City University
• Principal Investigator: WEI Min Osaka City University, 大学院・医学研究科, 講師 (70336783)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 腫瘍 / mircroRNA / 膀胱がん / microRNA

Research Abstract

The findings of the present study demonstrate that miR-125a and miR-125b are tumor-suppressor miRNAs and were down-regulated in the early stage of bladder carcinogenesis. We also identified 12 potential target genes of miR-125b by proteome analysis of pre-miR-125b-introuduced human bladder cells. These findings enhance our understanding of the function of miRNA, and provide potential chemoprevention and therapeutic targets for bladder cancer.

Research Products

• [Journal Article] Low-dose carcinogenicity of 2-amino-3-methylimidazo[4,5-f]quinoline in rats : Evidence for the existence of no-effect levels and a mechanism involving p21Cip/WAF1. (2011)
  • Author(s): Wei, M.
  • Journal Title: Cancer Sci. Volume: 102 Pages: 88-91
  • Peer Reviewed
• [Journal Article] Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis. (2011)
  • Author(s): Kakehashi, A., Wei, M.
  • Journal Title: Toxicol.Sci. Volume: 119 Pages: 61-72
  • Peer Reviewed
• [Journal Article] Non-genotoxic mode of action and possible threshold for hepatocarcinogenicity of Kojic acid in F344 rats. (2011)
  • Author(s): Chusiri, Y., Wei, M.
  • Journal Title: Food Chem Toxicol Volume: 49 Pages: 471-476
  • Peer Reviewed
• [Journal Article] Tumor-associated MUC5AC stimulates in vivo tumorigenicity of human pancreatic cancer. (2011)
  • Author(s): Hoshi, H., Wei, M.
  • Journal Title: International journal of oncology Volume: 38 Pages: 619-627
  • Peer Reviewed
• [Journal Article] Enhanced urinary bladder, liver and colon carcinogenesis in Zucker diabetic fatty rats in a mulri-organ carcinogenesis : Evidence for mechanisms involving activation of PI3K signaling and impairment of p53 on urinary bladder carcinogenesis. (2011)
  • Author(s): Ishii, N., Wei, M.
  • Journal Title: J Toxicol Pathol. Volume: 24 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Chemopreventive effects of 13alpha, 14alpha-epoxy-3beta-methoxyserratan-21beta-ol (PJJ-34), a serratane-type triterpenoid, in a rat multi-organ carcinogenesis bioassay. (2010)
  • Author(s): Doi, K., Sakai, K., Tanaka, R., Toma, K., Yamaguchi, T., Wei, M., Fukushima, S., Wanibuchi, H.
  • Journal Title: Cancer Lett. 289 Pages: 161-169
  • Peer Reviewed
• [Journal Article] Effects of pioglitazone, a peroxisome proliferator-activated receptor gamma agonist, on the urine and urothelium of the rat. (2010)
  • Author(s): Suzuki, S., Arnold, L. L., Pennington, K. L., Kakiuchi-Kiyota, S., Wei, M., Wanibuchi, H., Cohen, S.M.
  • Journal Title: Toxicol Sci. 113 Pages: 349-357
  • Peer Reviewed
• [Journal Article] Thresholds for genotoxic carcinogens : Evidence from mechanism-based carcinogenicity studies. (2010)
  • Author(s): Fukushima, S., Wei, M., Kakehashi, A., Wanibuchi, H.
  • Journal Title: Cancer Risk Assessment 8 Pages: 207-221
  • Peer Reviewed
• [Journal Article] Evaluation of the subchronic toxicity of dietary administered Equisetum arvense in F344 rats. (2010)
  • Author(s): Tago, Y., Wei, M., Ishii, N., Kakehashi, A., Wanibuchi, H.
  • Journal Title: J Toxicol.Pathol. 23 Pages: 245-251
  • Peer Reviewed
• [Journal Article] Chemopreventive effects of 13alpha,14alpha-epoxy-3beta-methoxyserratan-21beta-ol (PJJ-34), a serratane-type triterpenoid, in a rat multi-organ carcinogenesis bioassay. (2010)
  • Author(s): Doi, K., Wei, M.
  • Journal Title: Cancer Lett. Volume: 289 Pages: 161-169
  • Peer Reviewed
• [Journal Article] Effects of pioglitazone, a peroxisome proliferator-activated receptor gamma agonist, on the urine and urothelium of the rat. (2010)
  • Author(s): Suzuki, S., Wei, M.
  • Journal Title: Toxicol Sci. Volume: 113 Pages: 349-357
  • Peer Reviewed
• [Journal Article] Thresholds for genotoxic carcinogens : Evidence from mechanism-based carcinogenicity studies. (2010)
  • Author(s): Fukushima, S., Wei, M.
  • Journal Title: Cancer Risk Assessment Volume: 8 Pages: 207-221
  • Peer Reviewed
• [Journal Article] Evaluation of the subchronic toxicity of dietary administered Equisetum arvense in F344 rats. (2010)
  • Author(s): Tago, Y., Wei, M.
  • Journal Title: J Toxicol.Pathol. Volume: 23 Pages: 245-251
  • Peer Reviewed
• [Journal Article] Chemopreventive effects of 13alpha, 14alpha-epoxy-3beta-methoxyserratan-21beta-ol(PJJ-34),a serratane-type triterpenoid, in a rat multi-organ carcinogenesis bioassay. (2010)
  • Author(s): Doi, K., Wei, M., et al
  • Journal Title: Cancer Lett. 289 Pages: 161-169
  • Peer Reviewed
• [Journal Article] Enhancement of preneoplastic lesion yield by chios mastic Ggm in a rat liver medium-term carcinogenesis bioassay. (2009)
  • Author(s): Doi, K., Wei, M., Kitano, M., Uematsu, N., Inoue, M., Wanibuchi, H.
  • Journal Title: Toxicol.Appl.Pharmacol. 234 Pages: 135-142
  • Peer Reviewed
• [Journal Article] Ethanol dose not promote MeIQx-initiated rat colon carcinogenesis based on evidence from analysis of a colon cancer surrogate marker. (2009)
  • Author(s): Kushida, M., Wanibuchi, H., Wei, M., Kakehashi, A., Ozaki, K., Sukata, T., Miyata, K., Ogata, K., Uwagawa, S., Fukushima, S.
  • Journal Title: J Toxicol.Pathol. 22 Pages: 65-70
  • Peer Reviewed
• [Journal Article] Increased susceptibility to spontaneous lung cancer in mice lacking LIM-domain only 7. (2009)
  • Author(s): Tanaka-Okamoto, M., Hori, K., Ishizaki, H., Hosoi, A., Itoh, Y., Wei, M., Wanibuchi, H., Mizogchi, A., Nakamura, H., Miyoshi, J.
  • Journal Title: Cancer Sci 100 Pages: 608-616
  • Peer Reviewed
• [Journal Article] Existence of a threshold for the genotoxic carcinogens : Evidence from mechanism-based carcinogenicity studies. (2009)
  • Author(s): Fukushima, S., Kakehashi, A., Wei, M., Wanibuchi, H.
  • Journal Title: Genes and Environment 31 Pages: 33-36
  • Peer Reviewed
• [Journal Article] Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. (2009)
  • Author(s): Kakehashi, A., Inoue, M., Wei, M., Fukushima, S., Wanibuchi, H.
  • Journal Title: Toxicol.Appl.Pharmacol. 238 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Urinary bladder carcinogenesis induced by chronic exposure to persistent low-dose ionizing radiation after Chernobyl accident. (2009)
  • Author(s): Romanenko, A., Kakehashi, A., Morimura,K., Wanibuchi, H., Wei, M., Vozianov, A., Fukushima S.
  • Journal Title: Carcinogenesis 30 Pages: 1821-1831
  • Peer Reviewed
• [Journal Article] Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions. (2009)
  • Author(s): Kakehashi, A., Kato, A., Inoue, M., Ishii, N., Okazaki, E., Wei, M., Tachibana, T., Wanibuchi, H.
  • Journal Title: Toxicol.Appl.Pharmacol. 242 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Patassium bromated enhances N-Ethyl-N-Hydroxyethylnitrosamine-Induced kidney carcinogenesis only at high dose in wistar rats : Indication of the existence of an enhancement threshold. (2009)
  • Author(s): Wei, M., Hamoud, A.S., Yamaguchi, T., Kakehashi, A., Morimura, K., Doi, K., Kushida, M., Kitano, M., Wanibuchi, H., Fukushima, S.
  • Journal Title: Toxicol Pathol. 37 Pages: 983-991
  • Peer Reviewed
• [Journal Article] Enhancement of preneoplastic lesion yield by chios mastic Ggm in a rat liver medium-term carcinogenesis bioassay. (2009)
  • Author(s): Doi, K., Wei, M., et al
  • Journal Title: Toxicol.Appl.Pharmacol. 234 Pages: 135-142
  • Peer Reviewed
• [Journal Article] Ethanol dose not promote MeIQx-initiated rat colon carcinogenesis based on evidence from analysis of a colon cancer surrogate marker. (2009)
  • Author(s): Kushida, M., Wei, M., et al.
  • Journal Title: J Toxicol.Pathol 22 Pages: 65-70
  • Peer Reviewed
• [Journal Article] Increased susceptibility to spontaneous lung cancer in mice lacking LIM-domain only 7. (2009)
  • Author(s): Tanaka-Okamoto, M., Wei, M., et al.
  • Journal Title: Cancer Sci.
  • Peer Reviewed
• [Journal Article] Existence of a threshold for the genotoxic carcinogens : Evidence from mechanism-based carcinogenicity studies. (2009)
  • Author(s): Fukushima, S., Wei, M., et al.
  • Journal Title: Genes and Environment 31 Pages: 33-36
  • Peer Reviewed
• [Journal Article] Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. (2009)
  • Author(s): Kakehashi, A., Wei, M., et al
  • Journal Title: Toxicol.Appl.Pharmacol. 238 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Urinary bladder carcinogenesis induced by chronic exposure to persistent low-dose ionizing radiation after Chernobyl accident. (2009)
  • Author(s): Romanenko, A., Wei, M., et al
  • Journal Title: Carcinogenesis 30 Pages: 1821-1831
  • Peer Reviewed
• [Journal Article] Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions. (2009)
  • Author(s): Kakehashi, A, Wei, M., et al
  • Journal Title: Toxicol.Appl.Pharmacol. 242 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Patassium bromated enhances N-Ethyl-N-Hydroxyethylnitrosamine-Induced kidney carcinogenesis only at high dose in wistar rats : Indication of the existence of an enhancement threshold. (2009)
  • Author(s): Wei, M., et al
  • Journal Title: Toxicol Pathol. 37 Pages: 983-991
  • Peer Reviewed
• [Presentation] IQの低用量域における発がん性:閾値の存在 (2011)
  • Author(s): 魏民
  • Organizer: 第27回日本毒性病理学会総会
  • Place of Presentation: 大阪国際交流センター(招待講演)
  • Year and Date: 2011-01-27
• [Presentation] IQの発がん性には実際的な閾値が存在する (2010)
  • Author(s): 魏民
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(招待講演)
  • Year and Date: 2010-09-22
• [Presentation] ヒ素膀胱発がん原因物質の探索:新規ヒ素代謝物ジメチルモノチオアルシン酸の同定およびその膀胱上皮細胞に及ぼす影響の検討 (2010)
  • Author(s): 魏民、金川明裕、田尻正喜、山田貴宣、山野荘太郎、梯アンナ、鰐渕英機
  • Organizer: 第25回発癌病理研究会
  • Place of Presentation: 松島
  • Year and Date: 2010-08-24
• [Presentation] ヒ素膀胱発がん原因物質の探索:新規ヒ素代謝物ジメチルモノチオアルシン酸の同定およびその膀胱上皮細胞に及ぼす影響の検討 (2010)
  • Author(s): 魏民
  • Organizer: 第25回発癌病理研究会
  • Place of Presentation: 松島一の坊(招待講演)
  • Year and Date: 2010-08-24
• [Presentation] ヒトプロト型c-Ha-rasトランスジェニックラットを用いた浸潤性膀胱がんモデルの開発 (2010)
  • Author(s): 魏民、山野荘太郎、石井真美、梯アンナ、鰐渕英機
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-27
• [Presentation] ヒトプロト型c-Ha-rasトランスジェニックラットを用いた浸潤性膀胱がんモデルの開発 (2010)
  • Author(s): 魏民
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(招待講演)
  • Year and Date: 2010-04-27
• [Presentation] IQの発がん性には実際的な閾値が存在する (2010)
  • Author(s): 魏民、石井真美、北野光昭、多胡善幸、福島昭治、鰐渕英機
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] MicroRNA-125bはラット膀胱発がんの早期マーカーとして有用である (2009)
  • Author(s): 魏民
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-10-01
• [Presentation] 膀胱発がん物質の早期検出マーカーの検討 (2009)
  • Author(s): 魏民
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-05-02
• [Presentation] 膀胱尿路上皮におけるジメチルモノチオアルシン酸の影響 (2009)
  • Author(s): 魏民、圓藤吟史、鰐渕英機
  • Organizer: 第15回ヒ素シンポジウム
  • Place of Presentation: 大阪
• [Presentation] MicroRNA-125bはラット膀胱発がんの早期マーカーとして有用である (2009)
  • Author(s): 魏民、梯アンナ、山野荘太郎、石井真美、北野光昭、鰐渕英機
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 膀胱発がん物質の早期検出マーカーの検討 (2009)
  • Author(s): 魏民、梯アンナ、福島昭治、鰐渕英機
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都