CAMDI, a novel DISC1-binding protein, is required for radial migration

• Project/Area Number: 21790394
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: FUKUDA Toshifumi Tokyo University of Pharmacy and Life Science, 生命科学部, 助教 (50372313)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: CAMDI / Myosin / DISC1 / 神経 / 移動 / 脳 / 中心体 / 層構造 / 統合失調症 / 大脳皮質 / 神経細胞移動 / 子宮内遺伝子導入法

Research Abstract

This study has identified a novel disrupted in schizophrenia 1 (DISC1)-interacting protein, named CAMDI after coiled-coil protein associated with myosin II and DISC1. Knockdown of CAMDI revealed severely impaired radial migration. Interestingly, one single nucleotide polymorphism of the CAMDI gene (R828W) is identified. Furthermore, mice with overexpression of R828W in neurons exhibit an impaired radial migration. Our findings indicate that CAMDI is required for radial during neuronal development.

Research Products

• [Journal Article] A mitochondrial ubiquitin ligase MITOL controls cell toxicity of polyglutamine-expanded protein. (2011)
  • Author(s): Sugiura, A., Yonashiro, R., Fukuda, T., Matsushita, N., Nagashima, S., Inatome, R., Yanagi, S.
  • Journal Title: Mitochondrion 11 Pages: 139-146
• [Journal Article] Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms. (2011)
  • Author(s): Matsushita, N., Yonashiro, R., Ogata, Y., Sugiura, A., Nagashima, S., Fukuda, T., Inatome, R., Yanagi, S.
  • Journal Title: Genes Cells 16 Pages: 190-202
• [Journal Article] Phospholipase Cδ3 regulates RhoA/Rho kinase signaling and neurite outgrowth. (2011)
  • Author(s): Kouchi, Z., Igarashi, T., Shibayama, N., Inanobe, S., Sakurai, K., Yamaguchi, H., Fukuda, T. Yanag,i S., Nakamura, Y., Fukami, K.
  • Journal Title: J.Biol.Chem. 286 Pages: 8459-8471
• [Journal Article] Phospholipase C δ 3 regulates RhoA/Rho kinase signaling and neurite outgrowth (2011)
  • Author(s): Kouchi, Z
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 8459-8471
  • Peer Reviewed
• [Journal Article] Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms (2011)
  • Author(s): Matsushita, N.
  • Journal Title: Genes Cells Volume: 16 Pages: 190-202
  • Peer Reviewed
• [Journal Article] A mitochondrial ubiquitin ligase MITOL controls cell toxicity of polyglutamine-expanded protein (2011)
  • Author(s): Sugiura, A.
  • Journal Title: Mitochondrion Volume: 11 Pages: 139-146
  • Peer Reviewed
• [Journal Article] CAMDI, a novel disrupted in schizophrenia 1 (DISC1)-binding protein, is required for radial migration. (2010)
  • Author(s): Fukuda, T.^*, Sugita, S., Inatome, R., Yanagi, S.^*
  • Journal Title: J.Biol.Chem.(Corresponding author) 285 Pages: 40554-40561
• [Journal Article] CAMDI, a novel DISC1-binding protein, is required for radial migration (2010)
  • Author(s): Fukuda T.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 40554-40561
  • Peer Reviewed
• [Journal Article] Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced ROS generation. (2009)
  • Author(s): Yonashiro, R., Sugiura, A., Miyachi, M., Fukuda, T., Matsushita, N., Inatome, R., Ogata, Y., Suzuki, T., Dohmae, N., Yanagi, S.
  • Journal Title: Mol.Biol.Cell 20 Pages: 4524-4530
• [Journal Article] Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced ROS generation. (2009)
  • Author(s): Yonashiro, R.
  • Journal Title: Mol.Biol.Cell 20 Pages: 4524-4530
  • Peer Reviewed
• [Presentation] CRAGによるポリグルタミン病遺伝子治療の分子メカニズム (2010)
  • Author(s): 長島駿
  • Organizer: BMB 2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] CAMDIノックアウトマウスの解析of CAMDI knockout mouse. (2010)
  • Author(s): 柳澤香菜、福田敏史、服部晶、稲留涼子、柳茂
  • Organizer: BMB 2010 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] CAMDIのエンドサイトーシスとリサイクリングにおける機能解析Functional analysis of CAMDI with endocytosis and recycling. (2010)
  • Author(s): 井上晴満、福田敏史、角千春、稲留涼子、柳茂
  • Organizer: BMB 2010 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] CAMDIノックアウトマウスの解析 (2010)
  • Author(s): 柳澤香菜
  • Organizer: BMB 2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] CAMDIのエンドサイトーシスとリサイクリングにおける機能解析 (2010)
  • Author(s): 井上晴満
  • Organizer: BMB 2010第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 新規DISC1結合蛋白質CAMDIによる神経細胞移動の制御機構の解析 (2010)
  • Author(s): 福田敏史、杉田智子、稲留涼子、柳茂
  • Organizer: Neuro 2010第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] 新規DISC1結合タンパク質CAMDIによる神経細胞移動の制御機構の解析 (2010)
  • Author(s): 福田敏史
  • Organizer: Neuro 2010 第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] CRAGによるポリグルタミン病遺伝子治療の分子メカニズムMolecular mechanism of CRAG gene therapy for polyglutamine disease. (2010)
  • Author(s): 長島駿、福田敏史、岩瀬彩香、三浦恒平、稲留涼子、柳茂
  • Organizer: BMB 2010 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 新規DISC1結合蛋白質CAMDIによる中心体成熟と神経細胞移動の制御 (2009)
  • Author(s): 福田敏史、杉田智子、稲留涼子、柳茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 新規DISC1結合蛋白質CAMDI による大脳皮質神経細胞移動の制御機構の解析 (2009)
  • Author(s): 杉田智子、福田敏史、稲留涼子、柳茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 新規GTPaseタンパクCRAGによる公算か機構の解明 (2009)
  • Author(s): 窪田有花、長島駿、楊立偉、柳澤香菜、井上晴満、角千春、稲留涼子、福田敏史、柳茂
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 新規GTPaseタンパクCRAGによる抗酸化機構の解明 (2009)
  • Author(s): 窪田有花
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-09
• [Presentation] 新規DISC1結合蛋白質CAMDIによるMyosin II依存的な中心体の制御を介した大脳皮質形成機構の解析 (2009)
  • Author(s): 福田敏史、柳茂
  • Organizer: 第82回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 新規DISC1結合蛋白質CAMDIによるMyosin II依存的な中心体の制御を介した大脳皮質形成機構の解析 (2009)
  • Author(s): 福田敏史
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: ポートピアホテル(神戸市)
  • Year and Date: 2009-10-22
• [Remarks] ホームページ等