| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
CD133is a putative cancer stem cell marker of several malignancies but its roles still remain unclear. We here examined roles of CD133in tumorigenesis and stemness of human colon cancer cells. Knocked-down of CD133retarded tumor formation of colon cancer cells in nude mice and suppressed anchorage-independent cell growth. CD133-knockdown reduced transactivation activity ofβ-catenin resulting from AKT inactivation ; subsequently accelerated the enterocyte differentiation of colon cancer cells upon sodium butyrate treatment. Furthermore, we found a novel transcriptional regulation of CD133gene by GATA6transcription factor, which is bound to the upstream region of exon2of CD133gene. These data collectively suggest that CD133is involved in keeping undifferentiated-status and tumorigenesis of colon cancer.
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