Investigation of transcription factors that regulate development of Plasmodium gametocytes.

• Project/Area Number: 21790403
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Mie University
• Principal Investigator: KANEKO Izumi Mie University, 大学院・医学系研究科, 助教 (20515720)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: マラリア / 転写因子

Research Abstract

We identified two transcription factors of malaria parasites (AP2-G1 and Ap2-G2) that regulate sexual development of gametocytes. We investigated binding sites of these transcription factors on the genome with ChIP-seq (Chromatin immunoprecipitation followed by high-throughput DNA sequencing) and identified candidate sequences for their cis-acting elements. Our study indicated that these transcription factors regulate gene expression independently or cooperatively and suggested that other regulatory factors are involved in the gene regulation by these transcription factors.

Research Products

• [Journal Article] Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome. (2010)
  • Author(s): Iwanaga S, Khan SM, Kaneko I, Christodoulou Z, Newbold C, Yuda M, Janse CJ, Waters AP.
  • Journal Title: Cell Host Microbe 18;7(3) Pages: 245-255
  • Peer Reviewed
• [Journal Article] Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome. (2010)
  • Author(s): Iwanaga S, Khan SM, Kaneko I, Christodoulou Z, Newbold C, Yuda M, Janse CJ, Waters AP.
  • Journal Title: Cell Host Microbe Volume: 18;7(3) Pages: 245-55
  • Peer Reviewed
• [Journal Article] Transcription Factor AP2-Sp and its Target Genes in Malarial Sporozoites. (2009)
  • Author(s): Yuda M, Iwanaga S, Shigenobu S, Kato T,Kaneko I.
  • Journal Title: Molecular Microbiology. 75 Pages: 854-863
  • Peer Reviewed
• [Journal Article] Transcription Factor AP2-Sp and its Target Genes in Malarial Sporozoites. (2009)
  • Author(s): Yuda M, Iwanaga S, Shigenobu S, Kato T, Kaneko I.
  • Journal Title: Molecular Microbiology. 75 Pages: 854-863
  • Peer Reviewed
• [Presentation] ChIP-seq法を用いたマラリア原虫転写因子AP2-Oの新規標的遺伝子の同定 (2010)
  • Author(s): 金子伊澄、加藤知美、岩永史朗、油田正夫
  • Organizer: 第79回寄生虫学会
  • Place of Presentation: 旭川市(大雪クリスタルホール)
• [Presentation] ChIP-seq法を用いたマラリア原虫転写因子AP2-Oの新規標的遺伝子の同定 (2010)
  • Author(s): 金子伊澄
  • Organizer: 寄生虫学会
  • Place of Presentation: 旭川市大雪クリスタルホール
• [Presentation] ChIP-seq法を用いたマラリア原虫転写因子結合部位のgenome wideな解析 (2009)
  • Author(s): 金子伊澄・岩永史朗・加藤知美・油田正夫
  • Organizer: 第78回寄生虫学会
  • Place of Presentation: 法政大学
• [Book] 感染症-ウイルス・細菌・寄生虫の感染戦略(実験医学、増刊) (2009)
  • Author(s): 油田正夫、金子伊澄
  • Publisher: 羊土社
• [Book] 実験医学、増刊「感染症-ウイルス・細菌・寄生虫の感染戦略」 (2009)
  • Author(s): 油田正夫、金子伊澄
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 熱帯マラリア原虫への新規な直接遺伝子導入法 (2011)
  • Inventor(s): 岩永史朗・油田正夫・金子伊澄
  • Industrial Property Rights Holder: 国立大学法人三重大学
  • Filing Date: 2011-03-26
  • Overseas
• [Patent(Industrial Property Rights)] 熱帯マラリア原虫への新規な直接遺伝子導入法 (2011)
  • Inventor(s): 岩永史朗・油田正夫・金子伊澄
  • Industrial Property Rights Holder: 国立大学法人三重大学
  • Filing Date: 2011-03-26
  • Overseas