Construction of genomic library from Malaria parasite using its artificial chromosome

• Project/Area Number: 21790406
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Mie University
• Principal Investigator: IWANAGA Shiroh Mie University, 大学院・医学系研究科, 准教授 (20314510)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マラリア / 人工染色体 / マラリア原虫 / 遺伝子ライブラリー / テロメア

Research Abstract

To develop the system for the identification of drug resistance gene from parasite, we generate the high-coverage genomic library using the Plasmodium artificial chromosome (PAC) from drug resistance parasite. The targeted drug resistance gene could be identified by drug-screening from the PAC library which was made from the drug resistance parasite.

Research Products

• [Journal Article] Functional Identification of the Plasmodium Centromere and Generation of a Plasmodium Artificial Chromosome. (2010)
  • Author(s): Iwanaga S, Khan SM., Kaneko I, Christodoulou Z, Newbold C, Yuda M, Janse CJ, Waters AP.
  • Journal Title: Cell, Host & Microbe. 7, 3 Pages: 245-245
  • Peer Reviewed
• [Journal Article] Transcription Factor AP2-Sp and its Target Genes in Malarial Sporozoites. (2010)
  • Author(s): Yuda M, Iwanaga S, Shigenobu S, Kato T, Kaneko I.
  • Journal Title: Mol Microbiol. 75, 4 Pages: 854-863
  • Peer Reviewed
• [Journal Article] Functional Identification of the Plasmodium Centromere and Generation of a Plasmodium Artificial Chromosome (2010)
  • Author(s): S.Iwanaga, M, yuda, et.al
  • Journal Title: Cell, host & microbe Volume: Vol7(掲載確定)
  • Peer Reviewed
• [Journal Article] Identification of a transcription factor in the mosquito-invasive stage of malaria parasites. (2009)
  • Author(s): Yuda M, Iwanaga S, Shigenobu S, Mair GR, Janse CJ, Waters AP, Kato T, Kaneko I.
  • Journal Title: Mol Microbiol. 71, 6 Pages: 1402-1414
  • Peer Reviewed
• [Journal Article] マラリアワクチン開発の現況 (2009)
  • Author(s): 岩永史朗、鎮西靖男
  • Journal Title: 医学の歩み 231 Pages: 3-3
• [Journal Article] Identification of a transcription factor in the mosquito-invasive stage of malaria parasites (2009)
  • Author(s): M.Yuda, S.Iwanaga, et.al
  • Journal Title: Mol Microbiol Volume: Vol71 Pages: 1402-1414
  • Peer Reviewed
• [Journal Article] Transcription Factor AP2-Sp and its Target Genes in Malarial Sporozoites (2009)
  • Author(s): M.Yuda, S.Iwanaga, et.al
  • Journal Title: Mol Microbiol Volume: Vol75 Pages: 854-863
  • Peer Reviewed
• [Presentation] Identification of Transcription factor of Malaria Sporozoite. (2010)
  • Author(s): S.Iwanaga, I.Kaneko, M.Yuda, Awaji
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Japan
• [Presentation] Identification of Transcription factor of Malaria Sporozoite. (2010)
  • Author(s): Shiroh Iwanaga
  • Organizer: The 10^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Hyogo, Japan(招待講演)
• [Presentation] Identification of Transcription factor of Malaria Sporozoite. (2009)
  • Author(s): S.Iwanaga, I.Kaneko, M.Yuda ers
  • Organizer: American Society of Tropical Medicine and Hygiene 58th Annual Meeting
  • Place of Presentation: Washington, D.C.USA
• [Presentation] Functional Characterization of the plasmodium centromere and generation of a plasmodium artificial chromosome. (2009)
  • Author(s): S.Iwanaga, M.Yuda, I.Kaneko, C.J.Janse, A.P.Waters
  • Organizer: American Society of Tropical Medicine and Hygiene 58th Annual Meeting
  • Place of Presentation: Washington, D.C.USA
• [Presentation] Generation of a Plasmodium Artificial Chromosome (2009)
  • Author(s): S.Iwanaga, Awaji
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Japan
• [Presentation] Functional Characterization of the plasmodium centromere and generation of a plasmodium artificial chromosome (2009)
  • Author(s): S.Iwanaga
  • Organizer: American Society of Tropical Medicine and Hygiene 58^<th> Annual Meeting
  • Place of Presentation: Washington, D.C.USA
• [Presentation] Generation of a Plasmodium Artificial Chromosome (2009)
  • Author(s): S.Iwanaga
  • Organizer: The 9^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan
• [Patent(Industrial Property Rights)] マラリア原虫人工染色体を用いた薬剤耐性遺伝子の迅速同定法および組換えマラリア原虫作製法 (2011)
  • Inventor(s): 岩永史朗・油田正夫・金子伊澄
  • Industrial Property Rights Holder: 国立大学三重大学
  • Filing Date: 2011-03-26
  • Overseas
• [Patent(Industrial Property Rights)] マラリア原虫人工染色体を用いた薬剤耐性遺伝子の迅速同定法および組換えマラリア原虫作製法 (2011)
  • Inventor(s): 岩永史朗・金子伊澄・油田正夫
  • Industrial Property Rights Holder: 三重大学
  • Filing Date: 2011-03-26
  • Overseas
• [Patent(Industrial Property Rights)] マラリア原虫人工染色体を用いた薬剤耐性遺伝子の迅速同定法 (2010)
  • Inventor(s): 岩永史朗・油田正夫
  • Industrial Property Rights Holder: 国立大学三重大学
  • Industrial Property Number: 2010-071688
  • Filing Date: 2010-03-26