Apicomplexa specific traffic pathway in protozoa

• Project/Area Number: 21790411
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: NAKANO Yumiko National Institute of Infectious Diseases, 寄生動物部, 主任研究官 (30321764)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 原虫 / small GTPase / メンブレントラフィック / Rab5 / アピコンプレキサ / Plasmodium falciparu / 感染症 / Plasmodium falciparum

Research Abstract

One of the small GTPase, PfRab5a, was previously reported that involvement of endocytosis of erythrocyte cytosol. This work shows that another Rab5 isotype, Rab5b, possesses conserved motif with myristoilation site in N-terminus. Rab5b homologue is presented in Plasmodium and Toxopasma but not in Cryptosporidium, Paramecium and Tetrahymena in protozoa classified in Alveolata. Although expression of PfRab5b was low among three Rab5 isotypes, PfRab5b was expressed in erythrocytic stage. Expression of recombinant protein of PfRab5b was effective when an artificial gene appropriate for E. coli K12 codon was used.

Research Products

• [Journal Article] Marked Amplification and Diversification of Products of ras Genes from Rat Brain, Rab GTPases, in the Ciliates Tetrahymena thermophila and Paramecium tetraurelia. (2010)
  • Author(s): Saito-Nakano.Y., Nakahara,T., Nakano,K., Nozaki,T .,Numata,O
  • Journal Title: The Journal of Eukaryotic Microbiology. 57 Pages: 389-399
• [Journal Article] Marked Amplification and Diversification of Products of ras Genes from Rat Brain, Rab GTPases, in the Ciliates Tetrahymena thermophila and Paramecium tetraurelia. (2010)
  • Author(s): Saito-Nakano, Y., Nakahara, T., Nakano, K., Nozaki, T., Numata, O.
  • Journal Title: The Journal of Eukaryotic Microbiology Volume: 57 Pages: 389-399
  • Peer Reviewed
• [Presentation] Diversity of vesi cular trafficking in phagocytic prot ozoa and significance of traffic in Entamoeba histolytica. (2011)
  • Author(s): Yumiko, Saito-Nakano, Kentaro, Nakano, Tomoyoshi, Nozaki
  • Organizer: The 45th Annu al Japan-U.S. Joint Conference on Pa rasitic Diseases : Intestinal and Fre e-Living Protozoan Parasites Meeting
  • Place of Presentation: 国立感染症研究所(東京都)
• [Presentation] Diversity of vesicular trafficking in phagocytic protozoa and significance of traffic in Entamoeba histolytica. (2011)
  • Author(s): Yumiko Saito-Nakano, Kentaro Nakano, Tomoyoshi Nozaki.
  • Organizer: The 45th Annual Japan-U.S.Joint Conference on Parasitic Diseases : Intestinal and Free-Living Protozoan Parasites Meeting
  • Place of Presentation: 国立感染症研究所(東京都)
• [Presentation] Genetic identif ication of drug resistance in Plasmo dium falciparum using archive blood smears. (2010)
  • Author(s): Yumiko Saito-Nakano
  • Organizer: The 7^<th> Taiwan-Japan Symposium on Immunization and Travel Medicine.
  • Place of Presentation: 台湾CDC(台湾国)
• [Presentation] Dive rsity and Significance of Vesicular Trafficking in Entamoeba histolytica. (2010)
  • Author(s): Yumiko Saito-Nakano, Mami Okada,Gil Mallari Penuliar, Yuki Hanadate, Carol, A., Gilchrist, Oswald Crasta, Wil liam, A., Petri,Jr., Zhangjun Fei, Nino Trapaidze, Tomoyoshi Nozaki
  • Place of Presentation: Holel le Crystal(カナダ国)
• [Presentation] Genetic identification of drug resistance in Plasmodium falciparum using archive blood smears. (2010)
  • Author(s): Yumiko Saito-Nakano
  • Organizer: The 7^<th> Taiwan-Japan Symposium on Immunization and Travel Medicine.
  • Place of Presentation: 台湾CDC(台湾国、台北市)
• [Presentation] Diversity and Significance of Vesicular Trafficking in Entamoeba histolytica. (2010)
  • Author(s): Yumiko Saito-Nakano, Mami Okada, Gil Mallari Penuliar, Yuki Hanadate, Carol A.Gilchrist, Oswald Crasta, William A.Petri, Jr., Zhangjun Fei, Nino Trapaidze, Tomoyoshi Nozaki.
  • Organizer: Amebiasis Montreal 2010 Workshop.
  • Place of Presentation: Holel le Crystal(カナダ国モントリオール市)