Roles for intracellular innate immune receptors in host responses to Listeria monocytogenes infection

• Project/Area Number: 21790416
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kyoto University
• Principal Investigator: TSUCHIYA Kohsuke Kyoto University, 医学研究科, 助教 (50437216)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: インフラマソーム / AIM2 / NLRP3 / ASC / リステリア / 細胞内寄生菌 / NLRC4 / 自然免疫 / カスパーゼ1 / DNA / Nod-like receptor

Research Abstract

Listeria monocytogenes invades the cytoplasm of macrophages and induces the activation of caspase-1 and the subsequent maturation of IL-1β and IL-18. Although apoptosis-associated speck-like protein containing a caspase-activating and recruitment domain (ASC), an adaptor protein of Nod-like receptors, has been shown to play an essential role in inducing this cellular response to L. monocytogenes, the mechanism has not been fully elucidated. In this study, we demonstrated that absent in melanoma 2 (AIM2), a cytosolic DNA receptor, plays an important role in the activation of caspase-1 during L. monocytogenes infection.

Research Products

• [Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumour necrosis factor alpha and interleukin 6 production in murine macrophages. (2011)
  • Author(s): Sylvia Daim、河村伊久雄、土屋晃介, 他8名
  • Journal Title: Journal of Medical Microbiology Volume: 60 Pages: 5182-591
  • Peer Reviewed
• [Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumour necrosis factor alpha and interleukin 6 production in murine macrophages (2010)
  • Author(s): Sylvia Daim、河村伊久雄、土屋晃介, 他8名
  • Journal Title: Journal of Medical Microbiology 60巻 Pages: 5182-5191
  • Peer Reviewed
• [Journal Article] PD-1-PDL-1 pathawy impairs Th1 immune response in the late stage of infection with Mycobacterium bovis bacillus Calmette-Guerin (2010)
  • Author(s): 酒井俊祐、河村伊久雄、岡崎拓、土屋晃介, 他2名
  • Journal Title: International Immunology 22巻 Pages: 912-925
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄, 他7名
  • Journal Title: The Journal of Immunology 185巻 Pages: 1186-1195
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2- and MyD88-dependent phosphatidylinositol 3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages (2010)
  • Author(s): Yanna Shen、河村伊久雄、野村卓正、土屋晃介, 他7名
  • Journal Title: nfection and Immunity 78巻 Pages: 2857-2867
  • NAID: 120002381103
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): Sita R.Dewamitta、野村卓正、河村伊久雄、原英樹、土屋晃介, 他8名
  • Journal Title: Infection and Immunity 78巻 Pages: 1884-1894
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenea. (2010)
  • Author(s): Sita R.Dewamitta、野村卓正、河村伊久雄、原英樹、土屋晃介, (他8名)
  • Journal Title: Infection and Immunity Volume: 78 Pages: 1884-1894
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2- and MyD88-dependent phosphatidylinositol 3-kinase and Racl activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages. (2010)
  • Author(s): Yanna Shen、河村伊久雄、野村卓正、土屋晃介, 他7名
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2857-2867
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytogenea. (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄, 他7名
  • Journal Title: The Jouraal of Immunology Volume: 185 Pages: 1186-1195
  • Peer Reviewed
• [Journal Article] PD-1-PDL-1 pathawy impairs Th1 immune response in the late stage of infection with Mycobacterium bovis bacillus Calmette-Guerin. (2010)
  • Author(s): 酒井俊祐、河村伊久雄、岡崎拓、土屋晃介, 他2名
  • Journal Title: International Immunology Volume: 22 Pages: 912-925
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpa in activation and interleukin-1 alpha secretion in macrophages infected with List eria monocytogenes. (2010)
  • Author(s): Dewamitta S.R., Nomura T., Kawamura I., Hara H., Tsuchiya K., Kurenuma T., Shen Y., Daim S., Yamamoto T., Qu H., Sakai S., Xu Y., Mitsuyama M.
  • Journal Title: Infection and Immunity 78 Pages: 1884-1894
  • Peer Reviewed
• [Journal Article] The RD1 locus in the Mycobacterium tuberculosis genome contributes to activation of caspase-1 via induction of potassium ion efflux in infected macrophages (2009)
  • Author(s): 暮沼武士、河村伊久雄、原英樹、内山良介、Daim S.、Dewamitta S.R.、井俊祐、土屋晃介, 他2名
  • Journal Title: Infection and Immunity 77巻(有) Pages: 3992-4001
  • Peer Reviewed
• [Journal Article] The RD1 locus in the Mycobacterium tuberculosis genome contributes to activation of caspase-1 via induction of potassium ion efflux in infected macrophages. (2009)
  • Author(s): Kurenuma T., Kawamura I., Hara H., Uchiyama R., Daim S., Dewamitta S.R., Sakai S., Tsuchiya K., Nomura T., Mitsuyama M.
  • Journal Title: Infection and Immunity 77 Pages: 3992-4001
  • Peer Reviewed
• [Presentation] Involvement of absent in melanoma 2 (AIM2) in inflammasome activation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): 土屋晃介(筆頭演者)
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県・淡路市
  • Year and Date: 2010-09-08
• [Presentation] Involvement of absent in melanoma 2 (AIM2) in inflammasome activation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄, 他6名
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2010-09-08
• [Presentation] Mechanism of inflammmasome activation upon infection with Listeria monocytogenes (2010)
  • Author(s): 土屋晃介(筆頭演者)
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-08-25
• [Presentation] Mechanism of inflammmasome activation upon infection with Listeria monocytogenes (2010)
  • Author(s): 土屋晃介、原英樹、野村卓正, 他5名
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-08-25
• [Presentation] Mechanism of inflammasome activation upon infection with Listeria monocytogenes (2010)
  • Author(s): 土屋晃介(筆頭演者)
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県・横浜市
  • Year and Date: 2010-03-27
• [Presentation] Mechanism of inflammasome activation upon infection with Listeria monocytogenes. (2010)
  • Author(s): Tsuchiya K., Hara H., Nomura T., Daim S., Dewamitta S.R., Kawamura I., Mitsuyama M.
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県・横浜市
  • Year and Date: 2010-03-27
• [Presentation] Mechanism of the inflammasome activation upon infection with Listeria monocytogenes (2009)
  • Author(s): 土屋晃介(筆頭演者)
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪府・大阪市
  • Year and Date: 2009-12-04
• [Presentation] Mechanism of the inflammasome activation upon infection with Listeria monocytogenes. (2009)
  • Author(s): Tsuchiya K., Hara H., Nomura T., Daim S., Dewamitta S.R., Kawamura I., Mitsuyama M.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪府・大阪市
  • Year and Date: 2009-12-04
• [Presentation] Mechanism of the inflammasome activation upon infection with Listeria monocytogenes (2009)
  • Author(s): 土屋晃介(筆頭演者)
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県・淡路市
  • Year and Date: 2009-09-10
• [Presentation] Mechanism of the inflammasome activation upon infection with Listeria monocytogenes. (2009)
  • Author(s): Tsuchiya K., Hara H., Nomura T., Daim S., Dewamitta S.R., Kawamura I., Mitsuyama M.
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県・淡路市
  • Year and Date: 2009-09-10
• [Remarks] ホームページ等
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1204/